[1]杨兰,李欠,冯彦梅,等.一测多评法测定川白芷药材中5种指标成分的含量[J].江苏农业学报,2020,(01):199-205.[doi:doi:10.3969/j.issn.1000-4440.2020.01.028]
　YANG Lan,LI Qian,FENG Yan-mei,et al.Simultaneous determination of five index components in Angelica dahurica var. formosana by QAMS[J].,2020,(01):199-205.[doi:doi:10.3969/j.issn.1000-4440.2020.01.028]
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一测多评法测定川白芷药材中5种指标成分的含量()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
199-205
栏目:
加工贮藏·质量安全
出版日期:
2020-02-29

文章信息/Info

Title:
Simultaneous determination of five index components in Angelica dahurica var. formosana by QAMS
作者:
杨兰1; 2; 李欠1; 2; 冯彦梅1; 2; 常宝勤1; 2; 马秀琴1; 2; 张瑜1; 2; 邱黛玉1; 2
(1.甘肃省干旱生境作物学重点实验室，甘肃兰州730070；2.甘肃农业大学农学院，甘肃兰州730070）
Author(s):
YANG Lan1; 2; LI Qian1; 2; FENG Yan-mei1; 2; CHANG Bao-qin1; 2; MA Xiu-qin1; 2; ZHANG Yu1; 2; QIU Dai-yu1; 2
(1.Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070， Chinan;2.College of Agronomy, Gansu Agricultural University, Lanzhou 730070， Chinan)
关键词:
川白芷; 一测多评法; 相对校正因子; 质量控制
Keywords:
Angelica dahurica var. formosana; quantitative analysis of multi-components by single-marker(QAMS) method; relative correction factors; quality control
分类号:
R286.0
DOI:
doi:10.3969/j.issn.1000-4440.2020.01.028
文献标志码:
A
摘要:
以欧前胡素为内标物，建立了测定川白芷药材中白当归素、佛手柑内酯、氧化前胡素、欧前胡素、异欧前胡素多种成分含量的一测多评方法。采用HPLC方法，色谱柱为ZORBAX Eclipse Plus C18 (4.6 mm×250.0 mm，5 μm)，以甲醇-水为流动相，等度洗脱，柱温30 ℃，检测波长为250 nm，流速为1 ml/min进行测定。以欧前胡素为内标物，建立川白芷药材中白当归素、佛手柑内酯、氧化前胡素、异欧前胡素的相对校正因子。川白芷药材中白当归素、佛手柑内酯、氧化前胡素、异欧前胡素的相对校正因子重现性良好，分别为0.407、0.844、0.847、0.625，一测多评法测定结果与外标法测定结果无显著差异。表明以欧前胡素为内标物建立川白芷药材多种成分的一测多评法测定结果准确性良好，方法可行，可作为川白芷药材的质量控制方法。
Abstract:
The quantitative analysis of multi-components by single-marker (QAMS) method for content determination of byakangelicin, bergapten, oxypeucedanin, imperatorin and isoimperatorin in Angelica dahurica was established by setting imperatorin as internal standard substance. The high performance liquid chromatography (HPLC) method was adopted, and the chromatographic column of ZORBAX Eclipse Plus C18 ( 4.6 mm×250.0 mm, 5 μm) was used. The mobile phase was composed of methanol and water in isocratic elution. The column temperature was controlled at 30 ℃, the flow speed was 1 ml/min, and the detection wavelength was 250 nm. The relative correction factors of imperatorin to other four components were established by setting imperatorin as internal standard substance. The relative correction factors of byakangelicin, bergapten, oxypeucedanin and isoimperatorin in Angelica dahurica were 0.407, 0.844, 0.847 and 0.625, which had a high reproducibility. There was no significant difference between the results of QAMS method and external standard method. The QAMS method established by setting imperatorin as internal standard substance is accurate and feasible, which can be used as a useful quality control method for Angelica dahurica.

参考文献/References:

[1]赵亮,曹红. 白芷有效成分提取工艺的优化及其中药材质量研究[J].现代生物医学进展,2011,11(14): 2759- 2766.
[2]崔秋兵,张艺,兰莎. 白芷镇痛作用物质基础研究[J].中国实验方剂学杂志,2010,16(12):102-104.
[3]周淑敏. 白芷香豆素的提取及其抑菌活性研究[J].食品工业,2014,35(3):141-144.
[4]王方,王灿. 白芷醇提物延缓皮肤衰老与抗氧化作用的相关性研究[J].中国药房,2012,23(7):599-602.
[5]韦玮,徐嵬,杨秀伟,等. 杭白芷醋酸乙酯部位化学成分研究[J].中草药,2016,47(15):2606-2613.
[6]LIANG W H, CHANG T W, CHARNG Y C. Effects of drying methods on contents of bioactive compounds and antioxidant activities of Angelica dahuricae[J]. Food Sci Biotechnol, 2018, 27(4):1085-1092.
[7]JIANG Y, LU X, PENG W, et al. Study on the influence of sulfur fumigation on chemical constituents of Angelica dahuricae Radix (Baizhi) [J]. Trop J Pharm Res, 2015, 14(5):815.
[8]KANG J, ZHOU L, SUN J, et al. Chromatographic fingerprint analysis and characterization of furocoumarins in the roots of Angelica dahuricae by HPLC/DAD/ESI-MSn technique[J]. J Pharmaceu Biomed, 2008, 47(4/5):778-785.
[9]邓改改,崔治家,杨秀伟. 川白芷根极性化学成分研究[J].中国中药杂志,2015,40(19):3805-3810.
[10]郑立辉,王鹏君,李伟,等. 白芷精油成分分析及清除DPPH自由基活性[J].食品科学,2014,35(14):180-183.
[11]朱艺欣,李宝莉,马宏胜,等. 白芷的有效成分提取、药理作用及临床应用研究进展[J].中国医药导报,2014, 11(31):159-162.
[12]肖永庆,李丽,游小琳,等. 白芷质量标准研究[J].中国中药杂志,2004(7):48-51.
[13]周冰,刘培,陈京,等. 不同产地白芷药材中香豆素类及多糖类化学成分的分析评价[J].南京中医药大学学报, 2015,31(1):68-73.
[14]WANG J, LIAN P, YU Q, et al. Purification, characterization and procoagulant activity of polysaccharides from Angelica dahuricae roots[J]. Chem Cent J, 2017, 11(1):17.
[15]FAN G, DENG R, ZHOU L, et al. Development of a rapid resolution liquid chromatographic method combined with chemometrics for quality control of Angelica dahuricae radix[J]. Phytochem Analysis, 2011, 23(4):299-307.
[16]马逾英,钟世红,贾敏如,等. 紫外分光光度法测定川白芷中总香豆素类成分的含量[J].华西药学杂志,2005 (2):159-160.
[17]马俊,曹赟,陈玲,等. 基于UPLC及一测多评法测定不同等级白芷药材中4种香豆素类成分[J].中药材,2018, 41(10):2372-2376.
[18]中华人民共和国国家药典委员会. 中华人民共和国药典[M].北京:中国医药科技出版社,2015:105-106.
[19]CUI L, ZHANG Y, SHAO W, et al. Analysis of the HPLC fingerprint and QAMS from Pyrrosia species[J]. Industrial Crops and Products, 2016, 85:29-37.
[20]黄广伟,杨素德,曹婧迪. 一测多评法测定复方白芷止痛胶囊中4种指标成分[J].中国中医药信息杂志,2018, 25(9):86-90.
[21]刘凯,魏颖,刘洋洋,等. “一测多评”法在中药质量评价中的研究进展[J].现代中药研究与实践,2013,27(6): 81-84.
[22]WANG L, ZHANG Y, SUN X, et al. Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker[J]. Journal of Liquid Chromatography & Related Technologies, 2016, 39(8):422-427.
[23]张红伟,张振凌.“一测多评”法在中药质量控制中的研究进展[J].海峡药学,2010,22(11):88-89.
[24]杨丽霞,陈勇强,孙舰. 基于两种一测多评法对尾叶香茶菜中四种二萜类成分的质量控制研究[J].山西中医学院学报,2018,19(6):27-30.
[25]魏凤环,吴桂欣,朱清利,等. 一测多评法测定湿疹软膏中欧前胡素、异欧前胡素的含量[J].中国实验方剂学杂志,2012,18(21):125-128.
[26]陆兔林,石上梅,蔡宝昌,等. 基于一测多评的中药多成分定量研究进展[J].中草药,2012,43(12):2525-2529.
[27]田刚,李超,吴菲,等. 基于一测多评法对小儿豉翘清热颗粒中9个成分的质量控制[J].药物分析杂志,2018, 38(11):1922-1930.
[28]李宇邦,肖凤霞,宋小欣,等. 一测多评法比较毛橘红与光橘红5种黄酮类成分含量[J].中草药,2018,49(2): 444-449.
[29]文乾映,龙芳,杨华,等. 中药质量控制中一测多评法的应用进展[J].中国药房,2014,25(23):2185-2188.
[30]杨芳,万丽,胡一晨,等. 一测多评法测定川白芷药材中3种香豆素成分的含量[J].中国中药杂志, 2012, 37 (7):956-960.
[31]江宇勤,王佳琪,罗婷,等. 一测多评法测定不同产地白芷饮片中4种香豆素成分含量[J].时珍国医国药, 2018,29(11):2616-2619.
[32]史洋,雷云,许海玉,等. 白芷中3个主要活性成分含量测定及其质量评价研究[J].中国中药杂志, 2015, 40 (5):915-919.
[33]冯向东,王戈,刘直臻,等. 高效液相色谱法同时测定元胡止痛片中6种活性成分的含量[J].中南药学, 2019, 17(2):243-246.
[34]陈晓萌,张迎春,林朔,等. 外翻肠囊法发现元胡止痛片吸收成分群的研究[J].中国中药杂志, 2012, 37 (13): 2005-2011.
[35]YOSHIYUKI K, HIROMICHI O. Histamine-releaseeffectors from angelica dahuricavar dahuricaroot[J]. J Nat Prod, 1997, 60(3):249.
[36]舒翔,叶小春,陈军,等. 高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427-429.

备注/Memo

备注/Memo:
收稿日期：2019-07-09基金项目：国家自然科学基金项目（31860102）；甘肃省自然科学基金项目（18JR3RA185）；甘肃农业大学盛彤笙科技创新基金项目（GSAU-STS-2037）；甘肃农业大学人才引进专项(GSAU-RCZX201704)作者简介：杨兰（1995-），女，甘肃天祝人，硕士研究生，从事中药分析研究。(E-mail) 2281988971@qq.com通讯作者：李欠，(E-mail) liqian1984@gsau.edu.cn; 邱黛玉，(E-mail) qiudy@g
更新日期/Last Update: 2020-03-13