[1]徐重新,刘敏,张霄,等.农药危害风险及其残留检测用广谱特异性抗体研究进展[J].江苏农业学报,2019,(02):489-496.[doi:doi:10.3969/j.issn.1000-4440.2019.02.033]
　XU Chong-xin,LIU Min,ZHANG Xiao,et al.Advances in the development of broad-specificity antibodies for pesticide residue determination[J].,2019,(02):489-496.[doi:doi:10.3969/j.issn.1000-4440.2019.02.033]
点击复制

农药危害风险及其残留检测用广谱特异性抗体研究进展()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
489-496
栏目:
综述
出版日期:
2019-04-30

文章信息/Info

Title:
Advances in the development of broad-specificity antibodies for pesticide residue determination
作者:
徐重新; 刘敏;  张霄;  刘媛;  张存政;  刘贤金
(江苏省农业科学院农产品质量安全与营养研究所/省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室/农业部农产品质量安全控制技术与标准重点实验室，江苏南京210014)
Author(s):
XU Chong-xin; LIU Min; ZHANG Xiao; LIU Yuan; ZHANG Cun-zheng; LIU Xian-jin
（Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology/Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture, Nanjing 210014, China）
关键词:
农产品; 农药残留; 广谱特异性抗体; 检测方法
Keywords:
agricultural products; pesticide residue; broad-specificity antibodies; detection methods
分类号:
TS207.5+3
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.033
文献标志码:
A
摘要:
农药残留是威胁农产品质量安全的关键危害因素之一，是农产品质量安全监管工作的重要对象。当前已被开发和投入使用的农药种类纷繁复杂，农药残留检测工作任务多、责任大，因此建立一套可用于农药残留检测用的免疫学快速检测方法一直是广大科研工作者努力探索的目标。本文分门别类梳理农产品中主要农药残留及其危害，探讨国内外抗体创制领域最新热点技术，并就农药残留检测用广谱特异性抗体研发和应用现状进行概述及展望，旨在为指导农药残留快速检测技术的研发提供参考。
Abstract:
Pesticide residue is one of the key risk factors threatening the quality and safety of agricultural products, and it is also the important object of agricultural products quality safety inspection and supervision work. The types of pesticides that have been developed and used in agricultural production are numerous and complicated, the inspection work has many tasks and heavy responsibilities. Therefore, the establishment of a set of rapid immunoassay methods for pesticide residue detection has been the goal of scientific research workers. In this paper, the main types of pesticide residues in agricultural products and the corresponding hazards for people and animals were reviewed, the latest hotspot technologies in the field of antibody preparation at the domestic and foreign were explored, and the development and application of broad-specificity antibodies for pesticide residue detection were summarized and prospected, in order to provide reference for guiding the research and development of the rapid detection technology used in pesticide residue analysis.

参考文献/References:

[1]周本新.农药功过谁人曾与评说[J].农药市场信息,2016(22):6-8.
[2]GRUNG M, LIN Y, ZHANG H, et al. Pesticide levels and environmental risk in aquatic environments in China-a review[J]. Environment International, 2015, 81: 87-97.
[3]王运儒，邓有展，陈永森，等. 广西荔枝农药残留现状及膳食风险评估[J]. 南方农业学报，2018，49（9）:1804-1810.
[4]李培武,张奇,丁小霞,等.食用植物性农产品质量安全研究进展[J].中国农业科学,2014,47(18):3618-3632.
[5]中华人民共和国农业部.农业部办公厅关于征集《农产品质量安全法》修改意见的函(EB/OL).(2014-11-24)
[2014-11-28].http://www.moa.gov.cn/govpublic/ncpzlaq/201411/t20141128_4256470.htm.
[6]GRIMALT S, DEHOUCK P. Review of analytical methods for the determination of pesticide residues in grapes[J]. Journal of Chromatography A, 2016, 1433: 1-23.
[7]WATANABE E, MIYAKE S, YOGO Y. Review of enzyme-linked immunosorbent assays (ELISAs) for analyses of neonicotinoid insecticides in agro-environments[J].Journal of Agricultural and Food Chemistry, 2013, 61:12459-12472.
[8]刘媛,余向阳,梁颖,等.农药广谱特异性抗体制备技术研究进展[J].江苏农业学报,2009,25(2):428-432.
[9]周喜应.略论农药与农业可持续发展[J].中国植保导刊,2014,34(6):67-70.
[10]YADAVA I C, DEVI N L, SYED J H, et al. Current status of persistent organic pesticides residues in air, water, and soil, and their possible effect on neighboring countries: a comprehensive review of India[J]. Science of the Total Environment, 2015, 511: 123-137.
[11]李敏,傅桂平,吕宁,等.高危害农药释义及对我国农药管理的启示[J].农药科学与管理,2016,37(6):4-10.
[12]郭晨.高危农药:健康和环境的杀手[J].生态经济,2016,32(7):6-9.
[13]BERENBAUM M R. Does the Honey Bee ‘Risk Cup’ runneth over? Estimating aggregate exposures for assessing pesticide risks to Honey Bees in agroecosystems[J]. Journal of Agricultural and Food Chemistry, 2016, 64: 13-20.
[14]周培学.近30年有机氯农药和重金属在中国北方啮齿类动物中的变化趋势[D].杭州:浙江工业大学,2015.
[15]SONGA E A, OKONKWO J O. Recent approaches to improving selectivity and sensitivity of enzyme-based biosensors for organophosphorus pesticides: a review[J]. Talanta, 2016, 155: 289-304.
[16]ZHANG W, ASIRI A M, LIU D, et al. Nanomaterial-based biosensors for environmental and biological monitoring of organophosphorus pesticides and nerve agents[J]. TrAC Trends in Analytical Chemistry, 2014, 54: 1-10.
[17]TU W, XU C, LU B, et al. Acute exposure to synthetic pyrethroids causes bioconcentration and disruption of the hypothalamus-pituitary-thyroid axis in zebrafish embryos[J]. Science of the Total Environment, 2016, 542: 876-885.
[18]杜洁,张怡,洪盼盼,等.拟除虫菊酯对斑马鱼氧化代谢相关基因表达影响[J].浙江工业大学学报,2016,44(3):334-339.
[19]CIMINO A M, BOYLES A L, THAYER K A, et al. Effects of neonicotinoid pesticide exposure on human health: a systematic review[J]. Environmental Health Perspectives, 2017, 125(2): 155-162.
[20]邱德文.生物农药的发展现状与趋势分析[J].中国生物防治学报,2015,31(5):679-684.
[21]JIN L, ZHANG H N, LU Y H, et al. Large-scale test of the natural refuge strategy for delaying insect resistance to transgenic Bt crops[J]. Nature Biotechnology, 2015, 33: 169-174.
[22]FANG M, ROBERT J K, PETER P M, et al. Bacterial diversity in rhizospheres of nontransgenic and transgenic corn[J]. Applied and Environmental Microbiology, 2005, 71(7): 4132-4136.
[23]BERNSTEIN I L, BERNSTEIN J A, MILLER M, et al. Immune responses in farm workers after exposure to Bacillus thuringiensis pesticides[J]. Environmental Health Perspectives, 1999, 107(7): 575-582.
[24]许卓斌,王旻.抗体与抗体药物的前世今生[J].自然杂志,2016,38(4):271-277.
[25]ZENG N, LI Y, DU M. Recent advances on modeling the lateral flow immunoassay[J]. Journal of Advances in Biomedical Engineering and Technology, 2015, 2: 46-50.
[26]王廷华,李官成.抗体理论与技术 [M]. 3版.北京:科学出版社,2013.
[27]贾慧娜,罗海玲.多克隆抗体制备方法的研究进展[J].中国草食动物科学,2012,S1:66-69.
[28]LIU J K H. The history of monoclonal antibody development-Progress, remaining challenges and future innovations[J]. Annals of Medicine and Surgery, 2014, 3(4): 113-116.
[29]WEINER G J. Building better monoclonal antibody-based therapeutics[J].Nature Reviews Cancer, 2015, 15: 361-370.
[30]SANG J K, YOUNGWOO P, HYO J H. Antibody engineering for the development of therapeutic antibodies [J]. Molecules and Cells, 2005, 20(1): 17-29.
[31]MORRISON S L, JOHNSON M J, HERZENBERG L A, et al. Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains[J].Proc Natl Acad Sci USA,1984, 81(21): 6851-6855.
[32]SMITH G P. Filamentous fusion phage:novel expression vectors that display cloned antigens on the virion surface[J]. Science, 1985, 228: 1315-1317.
[33]锡建中,高宝龙,王建平.广义新型抗体的研究进展[J].黑龙江畜牧兽医,2014(23):54-56.
[34]HOOGENBOOM H R. Selecting and screening recombinant antibody libraries[J]. Nature Biotechnology, 2005, 23(9): 1105-1116.
[35]何扩,张秀媛,杜欣军,等.基因工程抗体在食品安全检测中应用进展研究[J].中国粮油学报,2014,29(8):124-128.
[36]MERSICH C, JUNGBAUER A. Generation of bioactive peptides by biological libraries[J]. Journal of Chromatography B, 2008, 861(2): 160-170.
[37]QI H, LU H Q, QIU H J, et al. Phagemid vectors for phage display: properties, characteristics and construction [J]. Journal of Molecular Biology, 2012, 417(3): 129-143.
[38]LEDAGAARD L, KILSTRUP M, KARATT-VELLATT A, et al. Basics of antibody phage display technology[J]. Toxins, 2018, 10: 236-251.
[39]文孟棠,刘媛,闫 帅,等.抗拟除虫菊酯类农药广谱性单克隆抗体的研制及鉴定[J].分析化学,2014,42(9):1245-1251.
[40]ZHAO Y Y, LIANG Y, LIU Y,et al. Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay[J].Analytical Biochemistry, 2016, 502: 1-7.
[41]梁颖,刘媛,祝金凤,等.甲氧基有机磷杀虫剂广谱特异性抗体的制备[J].分析化学,2008,36(5):647-652.
[42]贺江,梁颖,樊明涛,等.噬菌体展示技术制备甲氧基有机磷农药抗独特型抗体[J].分析化学,2011,39(2):178-182.
[43]WANG S T, GUI W J, GUO Y R, et al. Preparation of a multi-hapten antigen and broad specificity polyclonal antibodies for a multiple pesticide immunoassay[J]. Analytica Chimica Acta, 2007, 587: 287-292.
[44]EUGENIA K, HOSSNY H.Enzyme inhibition and enzyme-linked immunosorbent assay methods for carbamate pesticide residue analysis in fresh produce[J]. Journal of Food Protection, 2002, 63(12): 1758-1760.
[45]王升吉,尚佑芬,赵玖华,等.2, 4- D人工抗原合成及2, 4- D类农药特异性多克隆抗体的研制[J].农业环境科学学报,2008,27(5):2052-2057.
[46]WANG C, WANG Z, JIANG W, et al. A monoclonal antibody-based ELISA for multiresidue determination of avermectins in milk[J]. Molecules, 2012, 17(6): 7401-7414.
[47]焦凌霞,徐茜茜,刘媛,等.基于同源建模与分子对接技术构建抗Bt Cry1类毒素单链抗体定点饱和突变库[J].现代食品科技,2016,32(3):12-17.
[48]DONG S, ZHANG C Z, ZHANG X, et al. Production and characterization of monoclonal antibody broadly recognizing Cry1 toxins by use of designed polypeptide as hapten[J]. Analytical Chemistry, 2016, 88: 7023-7032.
[49]DONG S, BO Z Y, ZHANG C Z, et al. Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library[J].Applied Microbiology and Biotechnology, 2018, 102: 3363-3374.
[50]XU C X, ZHANG X, Liu X Q, et al. Selection and application of broad-specificity human domain antibody for simultaneous detection of Bt Cry toxins[J]. Analytical Biochemistry, 2016, 512: 70-77.
[51]王姝婷.农药多簇人工抗原的免疫特异性及多残留免疫金技术[D].杭州:浙江大学,2009.
[52]JIN R Y, GUO Y R, WANG C M, et al. Development of a bispecific monoclonal antibody to pesticide carbofuran and triazophos using hybrid hybridomas[J].Journal of Food Science, 2009, 74(1):1-6.
[53]李永芳,雷红涛.食品中小分子有害物多组分广谱免疫分析研究进展[C]//广东省食品学会.“健康与安全”学术研讨会暨2015年广东省食品学会年会论文集.广州：中国学术期刊电子出版社，2015：133-142.
[54]骆爱兰,余向阳,张存政,等.拟除虫菊酯类农药多残留酶免疫分析方法的建立[J].中国农业科学,2005,38(2): 308-312
[55]LIU Y, LOU Y, XU D, et al. Production and characterization of monoclonal antibody for class-specific determination of O,O-dimethyl organophosphorus pesticides and effect of heterologous coating antigens on immunoassay sensitivity[J]. Microchemical Journal, 2009, 93: 36-42.
[56]周丽君,黄启欣,雷红涛,等.二乙氧基硫代磷酸酯类有机磷农药单链抗体的制备及广谱特异性[J].高等学校化学学报,2012,33(10):2269-2274.
[57]赵扬.通用有机磷农药抗原表位多肽、单域抗体的制备及应用[D].新乡:河南科技学院,2016.
[58]SCHNEIDER P, GOODROW M H, GEE S J, et al. A highly sensitive and rapid ELISA for the arylurea herbicides diuron, monuron, and linuron[J]. Journal of Agricultural and Food Chemistry, 1994, 42(2): 413-422.
[59]KARU A E, GOODROW M H, SCHMIDT D J, et al. Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron[J]. Journal of Agricultural and Food Chemistry, 1994, 42(2): 301-309.
[60]秦娜. O-(2-异丙氧基羰基苯基)硫代磷酰胺类农药多残留免疫分析技术研究[D].南京:南京农业大学,2010.
[61]任克维.芳氧苯氧丙酸酯类除草剂多残留酶联免疫分析方法研究[D].南京:南京农业大学,2011.
[62]肖陈贵,顾大勇,丁晶,等.环戊二烯类有机氯农药单克隆抗体的制备及鉴定[J].食品安全质量检测学报,2014,5(11):3425-3430.
[63]于姣.食品中阿维菌素类药物酶联免疫检测方法的研究[D].天津:天津科技大学,2010.
[64]CHEN M, DING S Y, WEN K, et al. Development of a fluorescence-linked immunosorbent assay for detection of avermectins using a fluorescent single-domain antibody[J]. Analytical Methods, 2015, 7(9): 3728-3734.
[65]刘媛,林曼曼,张霄,等.基因突变技术在抗体亲和力体外成熟中的应用[J].浙江大学学报(农业与生命科学版),2016,42(1):1-7.
[66]张兆威,李培武,张奇,等.农产品中黄曲霉毒素的时间分辨荧光免疫层析快速检测技术研究[J].中国农业科学,2014,47(18):3668-3674.

相似文献/References:

[1]江景勇,王会福,何玲玲,等.台州草莓农药残留风险评估[J].江苏农业学报,2017,(06):1408.[doi:doi:10.3969/j.issn.1000-4440.2017.06.030]
　JIANG Jing-yong,WANG Hui-fu,HE Ling-ling,et al.Risk assessment of pesticide residues in strawberry from Taizhou city[J].,2017,(02):1408.[doi:doi:10.3969/j.issn.1000-4440.2017.06.030]
[2]李文.基质对光谱法快速检测蔬菜中残留乙酰甲胺磷的影响[J].江苏农业学报,2018,(05):1169.[doi:doi:10.3969/j.issn.1000-4440.2018.05.028]
　LI Wen.Influence of matrix on rapid detection of acephate residue in vegetables by spectral technology[J].,2018,(02):1169.[doi:doi:10.3969/j.issn.1000-4440.2018.05.028]
[3]程金金,吴世文,陈小龙,等.桃脆片加工过程中3种农药残留动态[J].江苏农业学报,2021,(02):517.[doi:doi:10.3969/j.issn.1000-4440.2021.02.030]
　CHENG Jin-jin,WU Shi-wen,CHEN Xiao-long,et al.Dynamics of three pesticide residues during the processing of peach crisps[J].,2021,(02):517.[doi:doi:10.3969/j.issn.1000-4440.2021.02.030]
[4]沈燕,仲建锋,黄亚威,等.植物类中药材生产过程中质量安全研究进展[J].江苏农业学报,2022,38(01):268.[doi:doi:10.3969/j.issn.1000-4440.2022.01.032]
　SHEN Yan,ZHONG Jian-feng,HUANG Ya-wei,et al.Advances in the quality and safety of Chinese herbal medicines in the producing process[J].,2022,38(02):268.[doi:doi:10.3969/j.issn.1000-4440.2022.01.032]
[5]卞立平,孙爱东,孙晓明,等.基于区块链技术的农产品深度溯源系统建设思考和设计方案[J].江苏农业学报,2022,38(04):1092.[doi:doi:10.3969/j.issn.1000-4440.2022.04.028]
　BIAN Li-ping,SUN Ai-dong,SUN Xiao-ming,et al.Thought of agricultural product deep-traceability system construction and scheme design based on blockchain technology[J].,2022,38(02):1092.[doi:doi:10.3969/j.issn.1000-4440.2022.04.028]

备注/Memo

备注/Memo:
收稿日期：2018-07-01 基金项目：国家自然科学基金项目（31701724、31630061、31371778）；江苏省农业科学院自然科学基金项目（6111676） 作者简介：徐重新（1987-），男，湖南新田人，博士，助理研究员，从事食品质量安全规范与控制技术研究。（E-mail）hhxyxcx@163.com 通讯作者：刘贤金，（E-mail）jaasliu@jaas.ac.cn
更新日期/Last Update: 2019-05-05