[1]许园园,李晓刚,李慧,等.梨CDPK基因家族全基因组序列鉴定分析[J].江苏农业学报,2015,(03):659-666.[doi:10.3969/j.issn.1000-4440.2015.03.031]
　XU Yuan-yuan,LI Xiao-gang,LI Hui,et al.Identification of calcium-dependent protein kinase(CDPK) gene family in pear（Pyrus bretschneideri Rehd）[J].,2015,(03):659-666.[doi:10.3969/j.issn.1000-4440.2015.03.031]
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梨CDPK基因家族全基因组序列鉴定分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年03期
页码:
659-666
栏目:
园艺
出版日期:
2015-06-30

文章信息/Info

Title:
Identification of calcium-dependent protein kinase(CDPK) gene family in pear（Pyrus bretschneideri Rehd）
作者:
许园园; 李晓刚; 李慧; 蔺经; 常有宏
(江苏省农业科学院园艺研究所，江苏省高效园艺作物遗传改良重点实验室，江苏南京210014)
Author(s):
XU Yuan-yuan; LI Xiao-gang; LI Hui; LIN Jing; CHANG You-hong
(Institute of Horticulture,Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
梨; CDPK家族基因; 全基因组鉴定; 系统进化
Keywords:
pear; calcium-dependent protein kinase(CDPK) gene family; genome-wide identification; phylogenetic analysis
分类号:
S661.2
DOI:
10.3969/j.issn.1000-4440.2015.03.031
文献标志码:
A
摘要:
CDPK是植物特有的一类丝氨酸/苏氨酸型蛋白激酶，在介导植物生育信号和逆境信号转导中具有重要作用。为揭示CDPK基因在梨生长发育与抗逆防御机制中的作用，本研究通过生物信息学手段，结合梨基因组注释信息，鉴定梨基因组中CDPK家族基因成员，利用MEGA6.0程序进行多序列比对、分类并构建系统进化树；利用per1程序以及GSDS工具进行基因结构与保守性分析，为植物CDPK基因功能分析与利用提供依据。结果表明,本研究成功鉴定出26个CDPK家族成员，根据系统发育树分析，所有PbCDPK被分为4类；基因结构预测结果表明，大多数PbCDPK均含有6~7个内含子；且预测的PbCDPK氨基酸序列绝大多数含4个EF-hand功能域；为了深入分析梨与其他物种的同源进化关系，构建了梨与拟南芥、水稻的CDPK基因系统进化树，进化树聚类分析结果将所有的CDPK蛋白分为四类。综上所述，目前已初步获得26个梨CDPK基因，为在基因组范围内研究CDPK基因在梨生长发育与逆境响应中的功能奠定了基础。
Abstract:
Calcium-dependent protein kinases (CDPKs)， classified into one kind of Ser/Thr kinases in plants， play important roles in various physiological processes, including plant growth and development, abiotic and biotic stress responses and plant hormone signalling. The annotation information of the pear genome was analyzed using bioinformatics tools to identify the sequence information of CDPK family members. The MEGA 6.0 software was used for multiple sequence alignment, classification, and construction of the phylogenic tree. Per1 software and GSDS were adopted to analyze gene structure and conservation. 26 CDPK gene family members, named PbCDPK1-PbCDPK26, were successfully identified. Most PbCDPKs consisted of 6 to 7 introns, and contained four EF-hand domains. The phylogenetic relationship revealed that CDPK proteins were grouped into four categories. This study established a foundation for a genome-wide study of CDPK functions in the growth and development of pear under adverse environmental conditions.

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备注/Memo

备注/Memo:
收稿日期：2014-10-13 基金项目：江苏省农业科技自主创新基金项目[CX（14）5018]；国家自然科学基金项目（31372051）；江苏省博士后基金项目（1302006B） 作者简介：许园园(1985-)，女，山东潍坊人，博士，助理研究员，主要从事果树逆境生理与分子生物学。（Tel）15951956519；（E-mail）xuyuanyuan850903@hotmail.com 通讯作者：蔺经，（E-mail）lj84390224@126.com；常有宏，（E-mail）cyh@jaas.ac.cn
更新日期/Last Update: 2015-06-30