[1]李文良,毛立,杨蕾蕾,等.猪瘟病毒糖基化E2蛋白和E0蛋白的协同免疫保护作用[J].江苏农业学报,2015,(02):357-361.[doi:10.3969/j.issn.1000-4440.2015.02.021]
　LI Wen-liang,MAO Li,YANG Lei-lei,et al.Synergistic effect of glycosylated E2- and E0-based subunit vaccine of classical swine fever[J].,2015,(02):357-361.[doi:10.3969/j.issn.1000-4440.2015.02.021]
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猪瘟病毒糖基化E2蛋白和E0蛋白的协同免疫保护作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
357-361
栏目:
畜牧兽医·水产养殖
出版日期:
2015-04-30

文章信息/Info

Title:
Synergistic effect of glycosylated E2- and E0-based subunit vaccine of classical swine fever
作者:
李文良; 毛立; 杨蕾蕾; 张纹纹; 江杰元
（江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心，江苏南京210014）
Author(s):
LI Wen-liang; MAO Li; YANG Lei-lei; ZHANG Wen-wen; JIANG Jie-yuan
(Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014， China)
关键词:
猪瘟; 亚单位疫苗; E2蛋白; E0蛋白; 协同作用
Keywords:
classical swine fever; subunit vaccine; E2 protein; E0 protein; synergistic effect
分类号:
S858.285.3
DOI:
10.3969/j.issn.1000-4440.2015.02.021
文献标志码:
A
摘要:
为了研究杆状病毒表达的糖基化亚单位疫苗的协同免疫保护作用，将重组杆状病毒感染Sf9细胞制备猪瘟E2、E0重组蛋白，Western blot检测蛋白表达和糖基化情况。用重组蛋白单独或联合免疫家兔，检测血清中抗体水平变化。在首免后4周用猪瘟兔化弱毒疫苗C株攻毒家兔，监测其体温变化，并运用RT-PCR检测家兔脾脏中病毒RNA。结果表明，E2、E2+E0免疫组兔均可诱导产生高水平的抗体和中和抗体，但两组间差异不显著。攻毒后E2免疫组2/5兔出现轻热症状，1/5兔脾脏病毒阳性；E2+E0组兔均未出现发热症状，也未检测到C株病毒RNA。E0组不能诱导兔产生中和抗体，但也能提供部分保护（2/5）。可见，基于E2、E0的亚单位疫苗具有良好的免疫原性，且两者联合使用具有协同作用，有望成为新型亚单位疫苗的候选抗原。
Abstract:
Classical swine fever is one of the devastating diseases for pig industry and vaccination is critical for disease control. To study the synergistic effect of glycosylated E2 and E0 subunit vaccine, recombinant glycosylated E2 and E0 proteins were expressed with the recombinant baculoviruses infecting Sf9 cells, and identified by Western blot and de-glycosylation tests (PNGase F treatment). Rabbits were vaccinated with E2, E0 and E2+E0 proteins and boosted two weeks later. Antibody and neutralizing antibody in serum samples were determined by blocking ELISA and viral neutralization test. Four weeks post primary vaccination, rabbits were challenged with CSFV C-strain and body temperature was monitored. Spleens were collected from all rabbits to determine the viral RNA by RT-PCR. Results showed that the rabbits immunized with E2 and E2+E0 developed high-level CSFV-specific antibodies and neutralizing antibody. Two fifth of rabbits in E2 group exhibited mild fever and one fifth was positive for viral RNA in spleen. All rabbits in group E2+E0 were normal and free of virus RNA. Although E0 vaccination did not induce detectable antibody, it gave partial protection (2/5). The results indicate that the recombinant E2 and E0 proteins possess good immunogenicities and have synergistic effect, and can be a candidate vaccine against CSF.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期：2014-08-19 基金项目：江苏省农业科技自主创新基金项目[CX(12)5049] 作者简介：李文良(1984-)，男，河南开封人，博士，助理研究员，主要从事动物传染病防治和诊断技术研究。(Tel)025-84391135；(E-mail)kfliwenliang@163.com 通讯作者：江杰元，(Tel)025-84391135，(E-mail)jieyuanj57@gmail.com
更新日期/Last Update: 2015-04-30