[1]宋波,杨阿燕,文国琴,等.藏野驴源芽孢杆菌的分离及全基因组序列分析[J].江苏农业学报,2026,42(03):531-541.[doi:doi:10.3969/j.issn.1000-4440.2026.03.011]
　SONG Bo,YANG Ayan,WEN Guoqin,et al.Isolation and whole genome sequence analysis of a Bacillus strain from Equus kiang[J].,2026,42(03):531-541.[doi:doi:10.3969/j.issn.1000-4440.2026.03.011]
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藏野驴源芽孢杆菌的分离及全基因组序列分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
531-541
栏目:
植物保护
出版日期:
2026-03-31

文章信息/Info

Title:
Isolation and whole genome sequence analysis of a Bacillus strain from Equus kiang
作者:
宋波; 杨阿燕; 文国琴; 张廷富; 秦明森
(西华师范大学生命科学学院,四川南充637000)
Author(s):
SONG Bo; YANG Ayan; WEN Guoqin; ZHANG Tingfu; QIN Mingsen
(College of Life Science, China West Normal University, Nanchong 637000, China)
关键词:
藏野驴; 芽孢杆菌; 生防菌; 全基因组测序; 抑菌性
Keywords:
Equus kiang; Bacillus; biocontrol bacteria; whole-genome sequencing; bacteriostasis
分类号:
Q939.92
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.011
文献标志码:
A
摘要:
从藏野驴粪便中分离到1株具有生物防治效应的芽孢杆菌菌株TEB-1。本研究基于第三代测序技术对菌株TEB-1进行全基因组测序和多维生物信息学分析,并对测序数据进行基因组装、基因预测、功能注释,并采用antiSMASH等软件对次级代谢产物合成基因簇进行深度挖掘与生物合成潜能评估。采用菌丝生长速率法验证菌株TEB-1对2种植物病原真菌暹罗刺盘孢菌和多主棒孢菌的抑制作用,并对菌株TEB-1进行耐药性试验。结果显示,菌株TEB-1的基因组全长为4 102 747 bp,G+C含量为43.8%,包含4 065个编码基因,85个tRNA,30个rRNA基因。生物信息学分析结果显示,该菌株存在丰富的次级代谢产物合成基因簇及多种耐药基因。功能验证结果表明,该菌株无菌发酵液在含量2.5%、5.0%、7.5%、10.0%时对暹罗刺盘孢菌和多主棒孢菌均表现出不同程度的抑制能力,10.0%含量处理时抑菌率分别达51.4%和38.7%。K-B纸片扩散法试验结果显示,TEB-1菌株对氨苄西林、青霉素和林可霉素3种抗生素具有耐药性,对四环素、庆大霉素、氯霉素、红霉素、环丙沙星、复方新诺明及头孢曲松等7种抗生素高度敏感,表明TEB-1菌株兼具显著的抗真菌活性与可控耐药特性。本研究结果为将TEB-1菌株开发为环境友好型生物农药提供了理论依据。
Abstract:
A Bacillus strain TEB-1 with biocontrol effects was isolated from the feces of Equus kiang. This study conducted whole-genome sequencing and multi-dimensional bioinformatics analysis of strain TEB-1 based on third-generation sequencing technology. The sequencing data were processed through gene assembly, gene prediction, and functional annotation, with in-depth mining and biosynthetic potential evaluation of secondary metabolite synthesis gene clusters using software such as antiSMASH. The inhibitory effects of strain TEB-1 against two plant pathogenic fungi, Colletotrichum siamense and Corynespora cassiicola, were verified using the mycelial growth rate method, and drug resistance tests were performed on strain TEB-1. The results showed that the complete genome of strain TEB-1 was 4 102 747 bp in length, with a G+C content of 43.8%, containing 4 065 coding genes, 85 tRNAs, and 30 rRNA genes. Bioinformatics analysis revealed abundant secondary metabolite synthesis gene clusters and multiple drug resistance genes in this strain. Functional validation results indicated that the sterile fermentation broth of the strain exhibited varying degrees of inhibitory activity against Colletotrichum siamense and Corynespora cassiicola at concentrations of 2.5%, 5.0%, 7.5%, and 10.0%. At the 10.0% concentration, the inhibition rates reached 51.4% and 38.7%, respectively. K-B disk diffusion tests demonstrated that strain TEB-1 was resistant to the antibiotics ampicillin, penicillin, and lincomycin, while showing high sensitivity to tetracycline, gentamicin, chloramphenicol, erythromycin, ciprofloxacin, cotrimoxazole, and ceftriaxone. These findings indicate that strain TEB-1 possesses significant antifungal activity alongside controllable resistance characteristics. The results of this study provide a theoretical basis for developing strain TEB-1 into an environmentally friendly biopesticide.

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备注/Memo

备注/Memo:
收稿日期：2025-04-07基金项目：国家自然科学基金项目(32470532)作者简介：宋波(1978-),女,四川都江堰人,博士,副教授,研究方向为资源微生物开发与利用。(E-mail)songbo1@126.com
更新日期/Last Update: 2026-04-17