[1]胡修忠,刘爽,向敏,等.地衣芽孢杆菌JH41C的抑菌特性及全基因组测序分析[J].江苏农业学报,2026,42(02):373-381.[doi:doi:10.3969/j.issn.1000-4440.2026.02.016]
　HU Xiuzhong,LIU Shuang,XIANG Min,et al.Antibacterial characteristics and genome sequencing analysis of Bacillus licheniformis JH41C[J].,2026,42(02):373-381.[doi:doi:10.3969/j.issn.1000-4440.2026.02.016]
点击复制

地衣芽孢杆菌JH41C的抑菌特性及全基因组测序分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
373-381
栏目:
畜牧兽医·水产养殖
出版日期:
2026-02-28

文章信息/Info

Title:
Antibacterial characteristics and genome sequencing analysis of Bacillus licheniformis JH41C
作者:
胡修忠1; 刘爽2; 向敏1; 李筱雯2; 余婕1; 刘辰晖1; 钟朱夏1; 王定发1; 程蕾1
(1.武汉市农业科学院,湖北武汉430070；2.湖北华大瑞尔科技有限公司,湖北荆州434008)
Author(s):
HU Xiuzhong1; LIU Shuang2; XIANG Min1; LI Xiaowen2; YU Jie1; LIU Chenhui1; ZHONG Zhuxia1; WANG Dingfa1; CHENG Lei1
(1.Wuhan Academy of Agricultural Sciences, Wuhan 430070, China；2.Hubei Huada Real Science & Technology Co., Ltd., Jingzhou 434008, China)
关键词:
地衣芽孢杆菌; 抑菌特性; 全基因组测序; 次级代谢产物; 杆菌肽; 纤维素酶
Keywords:
Bacillus licheniformis; antibacterial properties; whole genome sequencing; secondary metabolites; bacitracin; cellulase
分类号:
S852.65
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.016
文献标志码:
A
摘要:
本研究旨在对分离获得的地衣芽孢杆菌JH41C的抑菌特性及全基因组进行系统分析。体外抑菌试验结果表明,JH41C菌株对产气荚膜梭菌、金黄色葡萄球菌具有明显的抑制作用,高效液相色谱试验结果证实,JH41C菌株能合成杆菌肽。产酶性能分析结果显示,JH41C菌株具有产生淀粉酶、纤维素酶的能力。全基因组测序结果显示,JH41C基因组大小为4 450 351 bp,G+C含量为45.94%,预测到4 387个编码基因。分析结果表明,JH41C菌株含有丰富的代谢相关基因及多种抗生素(如青霉素、头孢菌素等)合成相关基因。预测到12个次级代谢产物合成相关基因簇,其中包含4个非核糖体途径合成的基因簇。由研究结果可以看出,JH41C在抑制病原菌、提高饲料利用率及替代抗生素的应用方面具有较大潜力,可为开发新型微生态制剂提供理论支持。
Abstract:
This study presents a comprehensive analysis of the antimicrobial characteristics and whole genome of Bacillus licheniformis strain JH41C. The results of in vitro antimicrobial tests demonstrated that JH41C strain exhibited significant inhibitory effects against Clostridium perfringens and Staphylococcus aureus. High-performance liquid chromatography analysis confirmed that the JH41C strain produced bacitracin. The strain also secreted amylase and cellulase. The 4 450 351 bp genome (G+C content 45.94%) contained 4 387 predicted protein-coding genes, including numerous metabolic genes and biosynthetic gene clusters for antibiotics such as penicillins and cephalosporins. Twelve secondary-metabolite clusters were identified, four of which correspond to non-ribosomal peptide synthetase pathways. These findings highlight the potential of JH41C for pathogen control, enhanced feed utilization, and antibiotic substitution, offering a theoretical basis for next-generation microecological agents.

参考文献/References:

[1]任文义,程雨辰,何金童,等. 地衣芽孢杆菌的生物学功能及其作为青贮饲料添加剂的应用潜力[J]. 动物营养学报,2023,35(10):6269-6276.
[2]丁红霞,潘朝阳,孟凡丛,等. 地衣芽孢杆菌HDTN对817肉鸡生长性能及肠道菌群的影响[J]. 微生物学报,2024,64(7):2479-2492.
[3]LIN E R, CHENG Y H, HSIAO F S, et al. Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers[J]. Revista Brasileira de Zootecnia,2019,48:e20170298.
[4]纪霜,李慷,靳锡辰,等. 地衣芽孢杆菌对草鱼生长性能、肌肉品质及土腥味防控的影响[J]. 南方农业学报,2025，56(1):334-342.
[5]RAMIREZ-OLEA H, REYES-BALLESTEROS B, CHAVEZ-SANTOSCOY R A. Potential application of the probiotic Bacillus licheniformis as an adjuvant in the treatment of diseases in humans and animals:a systematic review[J]. Frontiers in Microbiology,2022,13:993451.
[6]HE H H, ZHANG Y P, SHI G Y, et al. Recent biotechnological advances and future prospective of Bacillus licheniformis as microbial cell factories[J]. Systems Microbiology and Biomanufacturing,2023,3(4):521-532.
[7]李铭晗,赵权. 鸡源地衣芽孢杆菌的分离鉴定及全基因组分析[J]. 吉林农业大学学报,2022,44(5):613-623.
[8]周魏,余金凤,TAKRIFF M S,等. 一株产低温蛋白酶地衣芽胞杆菌NWMCC0046全基因组测序及分析[J]. 微生物学杂志,2023,43(6):42-53.
[9]DINDHORIA K, KUMAR S, BALIYAN N, et al. Bacillus licheniformis MCC 2514 genome sequencing and functional annotation for providing genetic evidence for probiotic gut adhesion properties and its applicability as a bio-preservative agent[J]. Gene,2022,840:146744.
[10]MUSSAKHMETOV A, KIRIBAYEVA A, DANIYAROV A, et al. Genome sequence and assembly of the amylolytic Bacillus licheniformis T5 strain isolated from Kazakhstan soil[J]. BMC Genomic Data,2024,25(1):3.
[11]RAPHEL S, HALAMI P M. Genome mining of Bacillus licheniformis MCC2514 for the identification of lasso peptide biosynthetic gene cluster and its characterization[J]. Archives of Microbiology,2024,206(4):143.
[12]胡修忠,凌明湖,王定发,等. 牛SPAG11D基因的原核表达及其抑菌活性研究[J]. 湖北农业科学,2015,54(5):1135-1138.
[13]HORNG Y B, YU Y H, DYBUS A, et al. Antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens[J]. AMB Express,2019,9(1):188.
[14]VAN DER VEKEN W, HAUTEKIET V, KIMMINAU E A, et al. Efficacy of probiotic Bacillus licheniformis DSM 28710 on performance and the mitigation of Clostridium perfringens-induced necrotic enteritis in broiler chickens[J]. Journal of Applied Animal Nutrition,2021,9(1):1-8.
[15]SONG M D, TANG Q, DING Y K, et al. Staphylococcus aureus and biofilms:transmission,threats,and promising strategies in animal husbandry[J]. Journal of Animal Science and Biotechnology,2024,15(1):44.
[16]王育丹,霍美霞,刘海燕,等. 杆菌肽在兽医临床的研究进展[J]. 中国兽医杂志,2024,60(2):83-88.
[17]姜立春,付丹阳,赵欣悦,等. 麦洼牦牛粪便纤维素降解菌的筛选、鉴定及产酶条件优化[J]. 江苏农业科学,2024,52(7):223-230.
[18]王文凡,刘银秀,谢晓杰,等. 牛粪堆肥中纤维素高效降解菌的筛选与产酶条件优化[J]. 微生物学通报,2023,50(11):4796-4811.
[19]张慧,赵恪,林陈强,等. 纤维素降解菌的分离筛选及产酶条件优化[J]. 福建农业学报,2023,38(9):1117-1123.
[20]张智,王蒙爱,冯丽荣,等. 纤维素酶产生菌的筛选及功能菌NF-101的全基因分析与产酶工艺优化[J]. 现代食品科技,2024,40(11):107-118.
[21]ELSABABTY Z E, ABDEL-AZIZ S H, IBRAHIM A M, et al. Purification,biochemical characterization,and molecular cloning of cellulase from Bacillus licheniformis strain Z9 isolated from soil[J]. Journal,Genetic Engineering & Biotechnology,2022,20(1):34.
[22]尹鹏飞,王继卿,郝志云,等. 开食料中添加纤维素酶对羔羊生长性能、瘤胃发酵和血清生化指标的影响[J]. 动物营养学报,2024,36(5):3170-3182.
[23]KHADEMI A R, HASHEMZADEH F, KHORVASH M, et al. Use of exogenous fibrolytic enzymes and probiotic in finely ground starters to improve calf performance[J]. Scientific Reports,2022,12(1):11942.
[24]SO S, WANAPAT M, CHERDTHONG A. Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14,cellulase and molasses on feed utilization,ruminal ecology and milk production of mid-lactating Holstein Friesian cows[J]. Journal of the Science of Food and Agriculture,2021,101(11):4481-4489.

相似文献/References:

[1]苏赵,胡凯弟,朱佳雯,等.地衣芽孢杆菌B-1降解西维因的环境条件[J].江苏农业学报,2018,(03):585.[doi:doi:10.3969/j.issn.1000-4440.2018.03.015]
　SU Zhao,HU Kai-di,ZHU Jia-wen,et al.Environmental conditions of carbaryl degradation by Bacillus licheniformis B-1[J].,2018,(02):585.[doi:doi:10.3969/j.issn.1000-4440.2018.03.015]

备注/Memo

备注/Memo:
收稿日期：2025-02-28基金项目：湖北省重点研发计划项目(2023DJC145)作者简介：胡修忠(1980-),男,山东曹县人,博士,高级畜牧师,主要从事畜牧微生物的发掘与应用研究。(E-mail)hu_xiu_zhong@126.com通讯作者：程蕾,(E-mail)chenglei_011@126.com
更新日期/Last Update: 2026-03-16