[1]彭金凤,于健,付丹阳,等.花生RLCK-VII亚家族的全基因组鉴定、系统进化与表达模式分析[J].江苏农业学报,2026,42(02):225-239.[doi:doi:10.3969/j.issn.1000-4440.2026.02.002]
　PENG Jinfeng,YU Jian,FU Danyang,et al.Genome-wide identification, phylogenetic analysis and expression pattern analysis of the peanut RLCK-VII subfamily[J].,2026,42(02):225-239.[doi:doi:10.3969/j.issn.1000-4440.2026.02.002]
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花生RLCK-VII亚家族的全基因组鉴定、系统进化与表达模式分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
225-239
栏目:
遗传育种·生理生化
出版日期:
2026-02-28

文章信息/Info

Title:
Genome-wide identification, phylogenetic analysis and expression pattern analysis of the peanut RLCK-VII subfamily
作者:
彭金凤1; 于健1; 付丹阳1; 孙亚亚1; 陈圆圆2; 陈佳佳1
(1.江苏农林职业技术学院风景园林学院,江苏镇江212400；2.河南农业大学林学院,河南郑州450046)
Author(s):
PENG Jinfeng1; YU Jian1; FU Danyang1; SUN Yaya1; CHEN Yuanyuan2; CHEN Jiajia1
（1.College of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, China;2.College of Forestry, Henan Agricultural University, Zhengzhou 450046, China)
关键词:
花生; RLCK-VII; 系统发育分析; 表达模式; 基因复制
Keywords:
Arachis hypogaea L.; RLCK-VII; phylogenetic analysis; expression pattern; gene duplication
分类号:
S565.2
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.002
文献标志码:
A
摘要:
受体样胞质激酶VII亚家族(RLCK-VII)作为植物先天免疫的关键调节因子,同时参与植物发育过程与对非生物胁迫的响应。本研究对花生RLCK-VII亚家族进行了系统分析,以揭示其在进化、表达及功能上的特征。系统发育分析将该亚家族分为8个分支,所有成员均包含保守的Motif2和Motif7,表明其功能具有保守性。共线性分析结果显示,该家族主要通过染色体片段复制事件扩张,为其新功能与表型多样性提供了遗传基础。启动子顺式作用元件分析结果显示,所有基因均含有光响应元件,且脱落酸与茉莉酸甲酯响应元件出现频率较高,与其参与胁迫应答的功能相符。RLCK-VII-4和RLCK-VII-70在根和果皮中相对表达量较高。在胚胎发育后期,RLCK-VII-29和RLCK-VII-59相对表达量较高,这些基因与种子成熟过程相关。RLCK-VII-20和RLCK-VII-58等基因在花和子房柄组织中相对表达量较高。RLCK-VII-4、RLCK-VII-58和RLCK-VII-70在茎中的相对表达量显著高于其在根、叶、花中的相对表达量(P＜0.05),RLCK-VII-20在茎和花中的相对表达量显著高于其在根、叶中的相对表达量(P＜0.05)。在干旱胁迫下,多个基因相对表达量显著升高,其中RLCK-VII-26、RLCK-VII-30、RLCK-VII-59和RLCK-VII-92基因相对表达量升高5倍以上,RLCK-VII-52、RLCK-VII-71、RLCK-VII-83、RLCK-VII-87和RLCK-VII-92相对表达量升高10倍以上。油菜素内酯和脱落酸处理诱导RLCK-VII-11、RLCK-VII-5等基因相对表达量升高。RLCK-VII-1、RLCK-VII-7和RLCK-VII-22等基因相对表达量不受激素和非生物胁迫调控。综上,本研究系统解析了花生RLCK-VII亚家族的进化与表达模式,为深入理解其在植物免疫、生长发育及胁迫应答中的功能提供了理论依据。
Abstract:
The receptor-like cytoplasmic kinase VII subfamily (RLCK-VII) acts as a key regulator of plant innate immunity, and is also involved in plant developmental processes and abiotic stress responses. In this study, a systematic analysis of the peanut RLCK-VII subfamily was performed to reveal its evolutionary, expressional, and functional characteristics. Phylogenetic analysis divided this subfamily into eight clades, and all members contained conserved Motif2 and Motif7, indicating the conservation of their functions. Collinearity analysis showed that this family mainly expanded through chromosomal segment duplication events, which provided a genetic basis for its new functions and phenotypic diversity. Analysis of promoter cis-acting elements revealed that all genes contained light-responsive elements, and abscisic acid- and methyl jasmonate-responsive elements had high frequencies, which was consistent with their functions in stress responses. RLCK-VII-4 and RLCK-VII-70 showed relatively high expression levels in roots and peels. In the late stage of embryonic development, RLCK-VII-29 and RLCK-VII-59 had relatively high expression levels, and these genes were associated with the seed maturation process. Genes such as RLCK-VII-20 and RLCK-VII-58 exhibited relatively high expression levels in flowers and pedicels. The relative expression levels of RLCK-VII-4, RLCK-VII-58, and RLCK-VII-70 in stems were significantly higher than those in roots, leaves, and flowers (P＜0.05), while the relative expression levels of RLCK-VII-20 in stems and flowers were significantly higher than those in roots and leaves (P＜0.05). Under drought stress, the relative expression levels of multiple genes increased significantly, among which the expression levels of RLCK-VII-26, RLCK-VII-30, RLCK-VII-59, and RLCK-VII-92 increased more than five-fold, and the expression levels of RLCK-VII-52, RLCK-VII-71, RLCK-VII-83, RLCK-VII-87, and RLCK-VII-92 increased more than ten-fold. Brassinosteroid and abscisic acid treatments induced the increased expression of genes such as RLCK-VII-11 and RLCK-VII-5. The relative expression levels of genes including RLCK-VII-1, RLCK-VII-7, and RLCK-VII-22 were not regulated by hormones or abiotic stresses. In conclusion, this study systematically clarified the evolutionary and expression patterns of the peanut RLCK-VII subfamily, which provides a theoretical basis for further understanding its functions in plant immunity, growth and development, and stress responses.

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备注/Memo

备注/Memo:
收稿日期：2025-11-16基金项目：江苏省高等学校基础科学(自然科学)研究项目(24KJA180003)；镇江市创新能力建设计划项目(SS2024010)作者简介：彭金凤(1992-),女,新疆昌吉人,博士,讲师,主要从事植物保护和生物信息学研究。(E-mail)pjf912530@163.com通讯作者：陈佳佳,(E-mail)jiajiachen@jsafc.edu.cn
更新日期/Last Update: 2026-03-16