[1]杨柳,孙枫,高晓晓,等.江苏葫芦科作物上西瓜银斑驳病毒的侵染与分子鉴定[J].江苏农业学报,2026,42(01):41-50.[doi:doi:10.3969/j.issn.1000-4440.2026.01.005]
　YANG Liu,SUN Feng,GAO Xiaoxiao,et al.Infection and molecular identification of watermelon silver mottle virus in cucurbit crops in Jiangsu[J].,2026,42(01):41-50.[doi:doi:10.3969/j.issn.1000-4440.2026.01.005]
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江苏葫芦科作物上西瓜银斑驳病毒的侵染与分子鉴定()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年01期
页码:
41-50
栏目:
植物保护
出版日期:
2026-01-31

文章信息/Info

Title:
Infection and molecular identification of watermelon silver mottle virus in cucurbit crops in Jiangsu
作者:
杨柳; 孙枫; 高晓晓; 任春梅; 季英华
(江苏省农业科学院植物保护研究所,江苏南京210014)
Author(s):
YANG Liu; SUN Feng; GAO Xiaoxiao; REN Chunmei; JI Yinghua
(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
西瓜银斑驳病毒; 发生; 分布; 分子鉴定; 江苏分离物; 系统进化分析
Keywords:
watermelon silver mottle virus; occurrence; distribution; molecular identification; Jiangsu isolate; phylogenetic analysis
分类号:
S436
DOI:
doi:10.3969/j.issn.1000-4440.2026.01.005
文献标志码:
A
摘要:
西瓜银斑驳病毒(Watermelon silver mottle virus, WSMoV)是正番茄斑萎病毒属(Orthotospovirus)的重要成员,由蓟马传播,可侵染葫芦科、茄科等多种作物。为明确江苏蔬菜作物上WSMoV的侵染情况、分子特征及传播来源,本研究于2023-2024年在江苏省主要蔬菜产区采集218份疑似WSMoV感染的葫芦科作物样品,采用RT-PCR、克隆测序等技术进行病原鉴定,并将鉴定得到的WSMoV与美国国家生物信息中心数据库登录的WSMoV氨基酸序列和碱基序列进行相似性分析和系统进化树构建,分析江苏WSMoV的可能传播来源。结果表明,来自南京市江宁区、南通市海门区、徐州市贾汪区的10份黄瓜样品检测到WSMoV侵染,样品带毒率为4.59%。江苏3个地区WSMoV分离物N基因差异较大,相似度仅为91.91%。其中,徐州市贾汪区分离物(登录号PV177960)和南通市海门区分离物(登录号PV177961)序列一致,与分离物WSMoV-SJZ(登录号PQ073372)的相似度较高,并在系统进化树中聚为同一分支；而南京市江宁区分离物(登录号PV177962)与分离物THBC(登录号PQ310671)和分离物HNTG(OQ715365)相似度较高,并在系统进化树中聚为同一分支,说明感染江苏黄瓜的WSMoV可能来源于不同的地区。本研究首次报道了WSMoV在江苏蔬菜作物上的发生状况,对该病毒的传播、流行及防治策略制定具有重要意义。
Abstract:
The watermelon silver mottle virus (WSMoV) is an important member of the Orthotospovirus genus. It is transmitted by thrips and can infect many crops including those in the families Cucurbitaceae and Solanaceae. To clarify the infection status, molecular characteristics and transmission sources of WSMoV on vegetable crops in Jiangsu province, 218 samples of cucurbit crops suspected to be infected with WSMoV were collected from the main vegetable producing areas during 2023-2024. The pathogen was identified by RT-PCR, cloning and sequencing. The identified WSMoV sequences were compared with the amino acid sequences and base sequences of WSMoV registered in the National Center for Biotechnology Information database of the United States for similarity analysis and phylogenetic tree construction, and the possible transmission sources of WSMoV in Jiangsu were analyzed. The results showed that WSMoV infection was detected in 10 cucumber samples from Jiangning District of Nanjing, Haimen District of Nantong and Jiawang District of Xuzhou, with a virus carrying rate of 4.59%. The N genes of WSMoV isolates in the three regions of Jiangsu exhibited considerable divergence, and the similarity was only 91.91%. Among them, the sequences of Xuzhou Jiawang isolate (PV177960) and Nantong Haimen isolate (PV177961) were consistent, and the similarity with isolate WSMoV-SJZ (PQ073372) was higher, and they were clustered into the same branch in the phylogenetic tree. The Nanjing Jiangning isolate (PV177962) was highly similar to the isolate THBC (PQ310671) and the isolate HNTG (OQ715365), and clustered into the same branch in the phylogenetic tree, indicating that the WSMoV infecting Jiangsu cucumbers may come from different regions. This study provides the first report of WSMoV occurrence in vegetable crops in Jiangsu province, which is of great significance for the spread, prevalence and control strategy of the virus.

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备注/Memo

备注/Memo:
收稿日期：2025-03-03基金项目：国家西甜瓜产业技术体系项目(CARS-25-2025-G20)作者简介：杨柳(1984-),女,江苏徐州人,博士,助理研究员,主要从事蔬菜作物病毒病研究。(E-mail)suiyangy@126.com通讯作者：季英华,(Tel)025-84390394；(E-mail)jiyinghua@jaas.ac.cn
更新日期/Last Update: 2026-02-09