[1]王博,陈震霄,王壮伟,等.NaCl胁迫下不同耐盐性葡萄砧木根系分泌物中有机酸组分差异及调控耐盐性有机酸筛选[J].江苏农业学报,2025,(12):2430-2438.[doi:doi:10.3969/j.issn.1000-4440.2025.11.015]
　WANG Bo,CHEN Zhenxiao,WANG Zhuangwei,et al.Differences in organic acid components of root exudates among grapevine rootstocks with different salt tolerances under NaCl stress and screening of organic acids regulating salt tolerance[J].,2025,(12):2430-2438.[doi:doi:10.3969/j.issn.1000-4440.2025.11.015]
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NaCl胁迫下不同耐盐性葡萄砧木根系分泌物中有机酸组分差异及调控耐盐性有机酸筛选()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年12期
页码:
2430-2438
栏目:
园艺
出版日期:
2025-12-31

文章信息/Info

Title:
Differences in organic acid components of root exudates among grapevine rootstocks with different salt tolerances under NaCl stress and screening of organic acids regulating salt tolerance
作者:
王博; 陈震霄; 王壮伟; 王西成
(江苏省农业科学院果树研究所,江苏省高效园艺作物遗传改良重点实验室,江苏南京210014)
Author(s):
WANG Bo; CHEN Zhenxiao; WANG Zhuangwei; WANG Xicheng
(Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
关键词:
葡萄砧木; 根系分泌物; 有机酸; 耐盐性
Keywords:
grapevine rootstocks; root exudates; organic acids; salt tolerance
分类号:
S663.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.015
文献标志码:
A
摘要:
为探究有机酸组分调控葡萄砧木耐盐性的生理机制,本研究以耐盐性较强的葡萄砧木101-14和较弱的5BB为试验材料,采用水培收集法与土培原位收集法两种方式,设置NaCl胁迫处理,利用液质联用技术对有机酸进行定性和定量分析。结果表明,培养方式显著影响根系分泌物组分,NaCl胁迫下,水培收集法收集的葡萄砧木101-14与5BB根系分泌物中有机酸种类显著高于土培原位收集法(P＜0.05),且不同培养方式获得的根系分泌物中存在特异性有机酸。综合两种培养方式,无NaCl胁迫条件下,葡萄砧木101-14与5BB根系分泌物中有机酸含量存在显著差异,含量差异有机酸主要为DL-3-苯基乳酸、山楂酸和牛磺酸。NaCl胁迫条件下,葡萄砧木101-14根系分泌物中泛酸含量显著高于葡萄砧木5BB,而山楂酸含量则显著低于葡萄砧木5BB。NaCl胁迫条件下,葡萄砧木101-14根系分泌物中水杨酸、顺式-乌头酸和壬二酸含量显著高于无NaCl胁迫对照。综上,本研究结果揭示了不同耐盐性葡萄砧木根系分泌物中有机酸组分的差异,筛选出泛酸、水杨酸、顺式-乌头酸和壬二酸等可能调控葡萄耐盐性的候选有机酸,为阐明有机酸介导的葡萄耐盐生理机制提供了理论基础。
Abstract:
To explore the physiological mechanism of organic acid components regulating salt tolerance in grapevine rootstocks, this study used grapevine rootstocks 101-14 (with strong salt tolerance) and 5BB (with weak salt tolerance) as experimental materials, adopted two methods of hydroponic collection and in-situ collection, set up NaCl stress treatments, and used liquid chromatography-mass spectrometry (LC-MS) technology for qualitative and quantitative analysis of organic acids. The results showed that the culture method significantly affected the components of root exudates. Under NaCl stress, the types of organic acids in root exudates of 101-14 and 5BB collected by the hydroponic collection method were significantly higher than those collected by the in-situ co-llection method (P＜0.05), and there were specific organic acids in root exudates obtained by different culture methods. Combining the two culture methods, under non-NaCl stress conditions, there were significant differences in the contents of organic acids secreted by the roots of 101-14 and 5BB, and the organic acids with content differences were mainly DL-3-phenyllactic acid, maslinic acid and taurine. Under NaCl stress, the content of pantothenic acid in root exudates of 101-14 was significantly higher than that of 5BB, while the content of maslinic acid was significantly lower than that of 5BB. Under NaCl stress, the contents of salicylic acid, cis-aconitic acid and azelaic acid in root exudates of 101-14 were significantly higher than those of the non-NaCl stress control group. In conclusion, this study reveals the differences in organic acid components in root exudates of grapevine rootstocks with different salt tolerances, and identifies candidate organic acids, such as pantothenic acid, salicylic acid, cis-aconitic acid and azelaic acid, that regulate grape salt tolerance, thereby providing a theoretical basis for clarifying the physiological mechanism of grapevine salt tolerance mediated by organic acids.

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备注/Memo

备注/Memo:
收稿日期：2025-05-29基金项目：江苏省农业科技自主创新基金项目[CX(24)3054]作者简介：王博(1987-),女,吉林吉林人,博士,助理研究员,主要从事葡萄栽培与微生态研究。(E-mail)bobileilei@163.com通讯作者：王西成,(E-mail)wxcown@163.com
更新日期/Last Update: 2026-01-20