[1]张楠,韩光杰,刘琴,等.稻纵卷叶螟FOXO调控Cu/Zn SOD参与抗杆状病毒CnmeGV感染的机制[J].江苏农业学报,2025,(12):2334-2340.[doi:doi:10.3969/j.issn.1000-4440.2025.11.006]
 ZHANG Nan,HAN Guangjie,LIU Qin,et al.Mechanism by which Cnaphalocrocis medinalis FOXO regulates Cu/Zn SOD to mediate resistance to CnmeGV infection[J].,2025,(12):2334-2340.[doi:doi:10.3969/j.issn.1000-4440.2025.11.006]
点击复制

稻纵卷叶螟FOXO调控Cu/Zn SOD参与抗杆状病毒CnmeGV感染的机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年12期
页码:
2334-2340
栏目:
植物保护
出版日期:
2025-12-31

文章信息/Info

Title:
Mechanism by which Cnaphalocrocis medinalis FOXO regulates Cu/Zn SOD to mediate resistance to CnmeGV infection
作者:
张楠1韩光杰1刘琴1李传明1林曼曼1祁建杭2陆玉荣1夏杨1徐健1
(1.江苏里下河地区农业科学研究所/国家农业微生物扬州观测实验站,江苏扬州225007;2.扬州绿源生物化工有限公司,江苏扬州225008)
Author(s):
ZHANG Nan1HAN Guangjie1LIU Qin1LI Chuanming1LIN Manman1QI Jianhang2LU Yurong1XIA Yang1XU Jian1
(1.Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/National Experimental Station of Yangzhou for Agricultural Microbiology, Yangzhou 225007, China;2.Yangzhou Lvyuan Bio-chemical Co. Ltd., Yangzhou 225008, China)
关键词:
稻纵卷叶螟稻纵卷叶螟颗粒体病毒氧化应激FOXO转录因子铜/锌超氧化物歧化酶
Keywords:
Cnaphalocrocis medinalisCnaphalocrocis medinalis granulovirusoxidative stresstranscription factor FOXOcopper/zinc superoxide dismutase (Cu/Zn SOD)
分类号:
S435.112+.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.006
文献标志码:
A
摘要:
为阐明稻纵卷叶螟(Cnaphalocrocis medinalis)对稻纵卷叶螟颗粒体病毒(C. medinalis granulovirus, CnmeGV)的抵抗机制,本研究分析了稻纵卷叶螟转录因子FOXO和铜/锌超氧化物歧化酶(Copper/zinc superoxide dismutase,Cu/Zn SOD)对CnmeGV感染的响应机制。结果表明,稻纵卷叶螟幼虫脂肪体中Cu/Zn SOD表达水平高于血淋巴、中肠和马氏管等组织;CnmeGV感染后6~48 h,稻纵卷叶螟脂肪体中超氧化物歧化酶活性显著增加,FOXO和Cu/Zn SOD基因mRNA表达水平均显著上调,且抗氧化剂N-乙酰半胱氨酸(NAC)能抑制上调水平。FOXO抑制剂AS1842856和SOD抑制剂LCS-1都能显著增强病毒DNA复制水平,提高CnmeGV感染幼虫的死亡率。上述结果说明,FOXO和Cu/Zn SOD基因的激活程度依赖于氧化应激水平,FOXO和Cu/Zn SOD基因在稻纵卷叶螟幼虫的抗病毒防御中起到关键作用。此外,FOXO抑制剂还能显著削弱病毒感染对Cu/Zn SOD转录的诱导水平,且在Cu/Zn SOD基因启动子区鉴定出多个FOXO结合位点,说明FOXO能直接调控Cu/Zn SOD的表达。本研究结果为稻纵卷叶螟防治、昆虫与病毒互作机制研究提供了新方法。
Abstract:
To elucidate the resistance mechanism of Cnaphalocrocis medinalis to C. medinalis granulovirus (CnmeGV), this study analyzed the response mechanism of transcription factor FOXO and copper/zinc superoxide dismutase (Cu/Zn SOD) in C. medinalis to viral infection. The results showed that the expression level of Cu/Zn SOD in fat body of C. medinalis larvae was higher than those in hemolymph, midgut and Malpighian tube. At 6-48 h after CnmeGV infection, the activity of superoxide dismutase in the fat body of C. medinalis increased significantly, and the mRNA expression levels of FOXO and Cu/Zn SOD genes were significantly up-regulated, and the antioxidant N-acetylcysteine (NAC) could inhibit the up-regulation. Both FOXO inhibitor AS1842856 and SOD inhibitor LCS-1 could significantly enhance the level of viral DNA replication and increase the mortality of CnmeGV-infected larvae. The above results indicated that the activation of FOXO and Cu/Zn SOD genes depended on the level of oxidative stress, and FOXO and Cu/Zn SOD genes played key roles in the antiviral defense of C. medinalis larvae. In addition, FOXO inhibitors also significantly attenuated the induction level of Cu/Zn SOD transcription by viral infection, and multiple FOXO binding sites were identified in the Cu/Zn SOD promoter region, indicating that FOXO could directly regulate the expression of Cu/Zn SOD. The results of this study provide a new method for the control of C. medinalis and the study of insect-virus interaction mechanism.

参考文献/References:

[1]王凤英,张孝羲,翟保平. 稻纵卷叶螟的飞行和再迁飞能力[J]. 昆虫学报,2010,53(11):1265-1272.
[2]ZHANG S K, REN X B, WANG Y C, et al. Resistance in Cnaphalocrocis medinalis (Lepidoptera:Pyralidae) to new chemistry insecticides[J]. Journal of Economic Entomology,2014,107(2):815-820.
[3]DESHAYES C, SIEGWART M, PAURON D, et al. Microbial pest control agents:are they a specific and safe tool for insect pest management?[J]. Current Medicinal Chemistry,2017,24(27):2959-2973.
[4]XU J, LIU Q, LI C M, et al. Field effect of Cnaphalocrocis medinalis granulovirus (CnmeGV) on the pest of rice leaffolder[J]. Journal of Integrative Agriculture,2019,18(9):2115-2122.
[5]徐健,李传明,韩光杰,等. 颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及害虫种群增长的影响[J]. 江苏农业学报,2018,34(1):29-33.
[6]SCHWARZ K B. Oxidative stress during viral infection:a review[J]. Free Radical Biology and Medicine,1996,21(5):641-649.
[7]SANDER W J, FOURIE C, SABIU S, et al. Reactive oxygen species as potential antiviral targets[J]. Reviews in Medical Virology,2022,32(1):e2240.
[8]CAMINI F C, DA SILVA CAETANO C C, ALMEIDA L T, et al. Implications of oxidative stress on viral pathogenesis[J]. Archives of Virology,2017,162(4):907-917.
[9]KATSUMA S, KAWAOKA S, MITA K, et al. Genome-wide survey for baculoviral host homologs using the Bombyx genome sequence[J]. Insect Biochemistry and Molecular Biology,2008,38(12):1080-1086.
[10]YANASE T, HASHIMOTO Y, KAWARABATA T. Identification of insertion and deletion genes in Autographa californica nucleopolyhedrovirus variants isolated from Galleria mellonella,Spodoptera exigua,Spodoptera litura and Xestia c-nigrum[J]. Virus Genes,2000,21(3):167-177.
[11]HAN G J, LIU Q, LI C M, et al. Transcriptome sequencing reveals Cnaphalocrocis medinalis against baculovirus infection by oxidative stress[J]. Molecular Immunology,2021,129:63-69.
[12]KRAFCZYK N, KLOTZ L O. FOXO transcription factors in antioxidant defense[J]. IUBMB Life,2022,74(1):53-61.
[13]MARTINS R, LITHGOW G J, LINK W. Long live FOXO:unraveling the role of FOXO proteins in aging and longevity[J]. Aging Cell,2016,15(2):196-207.
[14]KANG X L, WANG Y P, LIANG W J, et al. Bombyx mori nucleopolyhedrovirus downregulates transcription factor BmFoxO to elevate virus infection[J]. Developmental & Comparative Immunology,2021,116:103904.
[15]TIAN Z Q, ZHA M, CAI L M, et al. FoxO-promoted peroxiredoxin1 expression induced by Helicoverpa armigera single nucleopolyhedrovirus infection mediates host development and defensive responses[J]. Ecotoxicology and Environmental Safety,2022,234:113414.
[16]LO H R, CHAO Y C. Rapid titer determination of baculovirus by quantitative real-time polymerase chain reaction[J]. Biotechnology Progress,2004,20(1):354-360.
[17]韩光杰,刘琴,李传明,等. 稻纵卷叶螟颗粒体病毒的持续感染及检测[J]. 中国农业科学,2020,53(19):3988-3995.
[18]KATSUMA S, KOBAYASHI J, KOYANO Y, et al. Baculovirus-encoded protein BV/ODV-E26 determines tissue tropism and virulence in lepidopteran insects[J]. Journal of Virology,2012,86(5):2545-2555.
[19]FENG G, YU Q, HU C, et al. Apoptosis is induced in the haemolymph and fat body of Spodoptera exigua larvae upon oral inoculation with Spodoptera litura nucleopolyhedrovirus[J]. The Journal of General Virology,2007,88(8):2185-2193.
[20]WANG Y, OBERLEY L W, MURHAMMER D W. Evidence of oxidative stress following the viral infection of two lepidopteran insect cell lines[J]. Free Radical Biology and Medicine,2001,31(11):1448-1455.
[21]SAH N K, TANEJA T K, PATHAK N, et al. The baculovirus antiapoptotic p35 gene also functions via an oxidant-dependent pathway[J]. Proceedings of the National Academy of Sciences of the United States of America,1999,96(9):4838-4843.
[22]SENGUPTA A, MOLKENTIN J D, PAIK J H, et al. FoxO trans-cription factors promote cardiomyocyte survival upon induction of oxidative stress[J]. The Journal of Biological Chemistry,2011,286(9):7468-7478.
[23]CHEN F M, CHEN L R, LIANG J L, et al. Potential role of superoxide dismutase 3 (SOD3) in resistance to influenza A virus infection[J]. Antioxidants,2023,12(2):354.
[24]KOBAYASHI Y, NOJIMA Y, SAKAMOTO T, et al. Comparative analysis of seven types of superoxide dismutases for their ability to respond to oxidative stress in Bombyx mori[J]. Scientific Reports,2019,9(1):2170.
[25]MATSUDA–IMAI N, KATSUMA S. Characterization of Bombyx mori nucleopolyhedrovirus infection in fat body-derived Bombyx mori cultured cells[J]. Journal of Invertebrate Pathology,2020,177:107476.
[26]SPELLBERG M J, MARR M T. FOXO regulates RNA interference in Drosophila and protects from RNA virus infection[J]. Procee-dings of the National Academy of Sciences of the United States of America,2015,112(47):14587-14592.
[27]SIM C, DENLINGER D L. Catalase and superoxide dismutase-2 enhance survival and protect ovaries during overwintering diapause in the mosquito Culex pipiens[J]. Journal of Insect Physiology,2011,57(5):628-634.

相似文献/References:

[1]王琳,包云轩,谢晓金,等.基于地统计法的稻纵卷叶螟时空变化特征[J].江苏农业学报,2017,(01):50.[doi:10.3969/j.issn.1000-4440.2017.01.008 ]
 WANG Lin,BAO Yun-xuan,XIE Xiao-jin,et al.Spatiotemporal characteristics of rice leaf rollers (Cnaphalocrocis medinalis) based on geostatistics method[J].,2017,(12):50.[doi:10.3969/j.issn.1000-4440.2017.01.008 ]
[2]徐健,李传明,韩光杰,等.颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及害虫种群增长的影响[J].江苏农业学报,2018,(01):29.[doi:doi:10.3969/j.issn.1000-4440.2018.01.004]
 XU Jian,LI Chuan-ming,HAN Guang-jie,et al.The effect of Cnaphalocrocis medinalis granulovirus (CnmeGV) on larva infection and population regulation of rice leaffolder[J].,2018,(12):29.[doi:doi:10.3969/j.issn.1000-4440.2018.01.004]
[3]郭铭淇,包云轩,黄璐,等.无人机多光谱影像在稻纵卷叶螟危害监测中的应用[J].江苏农业学报,2023,(07):1530.[doi:doi:10.3969/j.issn.1000-4440.2023.07.010]
 GUO Ming-qi,BAO Yun-xuan,HUANG Lu,et al.Application of multispectral image taken by unmanned aerial vehicle in monitoring Cnaphalocrocis medinalis Güenée damage on rice growth[J].,2023,(12):1530.[doi:doi:10.3969/j.issn.1000-4440.2023.07.010]

备注/Memo

备注/Memo:
收稿日期:2025-08-28基金项目:国家重点研发计划项目(2024YFD1400700);国家自然科学基金项目(32302351、32372539);农业基础性长期性科技工作项目(NAES069AM04);江苏省农业科技自主创新基金项目[CX(24)1005]作者简介:张楠(1992-),男,江苏泗洪人,博士,助理研究员,主要从事农业害虫生物防治工作。(E-mail)znfezhangnan@hotmail.com通讯作者:徐健,(E-mail)bio-xj@163.com
更新日期/Last Update: 2026-01-20