[1]李婵琼,易琼,曾科,等.不同时期施硒对水稻硒吸收及累积的影响[J].江苏农业学报,2025,(12):2298-2306.[doi:doi:10.3969/j.issn.1000-4440.2025.11.002]
　LI Chanqiong,YI Qiong,ZENG Ke,et al.Effects of selenium application at different growth stages on selenium uptake and accumulation in rice[J].,2025,(12):2298-2306.[doi:doi:10.3969/j.issn.1000-4440.2025.11.002]
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不同时期施硒对水稻硒吸收及累积的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年12期
页码:
2298-2306
栏目:
遗传育种·生理生化
出版日期:
2025-12-31

文章信息/Info

Title:
Effects of selenium application at different growth stages on selenium uptake and accumulation in rice
作者:
李婵琼1; 2; 易琼2; 曾科2; 丁武汉2; 唐拴虎2; 张木2
(1.仲恺农业工程学院资源与环境学院,广东广州510225；2.广东省农业科学院农业资源与环境研究所/农业农村部南方植物营养与肥料重点实验室/广东省养分资源循环利用与耕地保育重点实验室,广东广州510640)
Author(s):
LI Chanqiong1; 2; YI Qiong2; ZENG Ke2; DING Wuhan2; TANG Shuanhu2; ZHANG Mu2
(1.College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;2.Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Nutrient Cycling and Farmland Conservation, Guangzhou 510640, China)
关键词:
水稻; 硒; 累积; 转运蛋白基因; 酸性土壤
Keywords:
rice; selenium; accumulation; transporter gene; acidic soil
分类号:
S511.01
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.002
文献标志码:
A
摘要:
为探讨不同生育期施硒对华南酸性土壤条件下水稻硒富集的影响及水稻富集硒机制,给富硒大米的生产提供依据,本研究通过大田试验,设置不施硒对照(CK)、分蘖期施硒(T1)、拔节期施硒(T2)和灌浆期施硒(T3)4个处理,对水稻籽粒硒含量、各级叶片硒含量、各级茎秆硒含量、各级茎节硒含量、根硒含量、籽粒有机硒含量以及转运蛋白基因OsNRT1.1B相对表达量进行测定。结果表明,施硒显著提升了水稻籽粒硒含量,灌浆期施硒处理的籽粒有机硒含量较对照显著提升,且随着施硒时间的延迟,籽粒硒含量显著增加,而叶片硒含量则降低；施硒均显著提高了水稻茎秆硒含量,其中灌浆期施硒处理与拔节期施硒处理差别不大,但均显著高于分蘖期施硒处理；施硒降低了水稻籽粒硒累积量占比,但灌浆期施硒处理较分蘖期施硒处理和拔节期施硒处理显著提高了籽粒有机硒含量；灌浆期施硒处理相比其他施硒处理显著提升了转运蛋白基因OsNRT1.1B相对表达量,增加了硒从茎叶向籽粒的迁移系数。水稻生育期内硒肥后移可以减少硒在茎叶等营养器官的累积,促进硒向籽粒的转运与累积。
Abstract:
To investigate the effects of selenium application at different growth stages on selenium enrichment in rice under acidic soil conditions in South China and the mechanism of selenium accumulation in rice, and to provide a basis for the production of selenium-enriched rice, this study conducted field experiments with four treatments: no selenium application as control (CK), selenium application at the tillering stage (T1), selenium application at the jointing stage (T2), and selenium application at the filling stage (T3). Measurements were conducted on selenium content in rice grains, hierarchical leaf selenium content, hierarchical stem selenium content, hierarchical node selenium content, root selenium content, organic selenium content in grains, and the relative expression level of the transporter gene OsNRT1.1B. The results demonstrated that selenium application markedly enhanced grain selenium content. In particular, applying selenium at the filling stage led to a significant increase in organic selenium content compared to the control. Furthermore, delayed selenium application resulted in progressively higher grain selenium levels, whereas leaf selenium content exhibited a declining trend. Selenium application significantly increased the selenium content in rice stems. The selenium content in rice stems showed no significant diffe-rence between the T2 and T3 treatments. However, selenium content in rice stems under T2 and T3 treatments was significantly higher than that under T1 treatment. While selenium application reduced the proportion of selenium accumulation in rice grains, application at the filling stage significantly increased the organic selenium content in grains compared to applications at the tillering and jointing stages. Selenium application at the filling stage significantly up-regulated the relative expression level of the transporter gene OsNRT1.1B, thereby increasing the translocation factor of selenium from stems and leaves to the grains. Delaying selenium fertilizer application redirects selenium from being accumulated in vegetative organs to being translocated and accumulated in the grains.

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备注/Memo

备注/Memo:
收稿日期：2025-02-18基金项目：国家自然科学基金项目(42107374)；广东省省级科技计划项目(2022A0505020002)；广东省农业科学院中青年学科带头人培养计划项目(R2023PY-JG017)作者简介：李婵琼(1999-),女,广东化州人，硕士研究生,主要从事水稻硒富集机制研究。(E-mail)1803140468@qq.com通讯作者：张木,(E-mail)zhangmu@gdaas.cn
更新日期/Last Update: 2026-01-20