[1]纪林春,李悦,包红朵,等.肠道高黏附性噬菌体对小鼠沙门氏菌感染的预防作用[J].江苏农业学报,2025,(09):1844-1851.[doi:doi:10.3969/j.issn.1000-4440.2025.09.019]
　JI Linchun,LI Yue,BAO Hongduo,et al.The preventive effect of a gut-adherent bacteriophage against Salmonella infection in mice[J].,2025,(09):1844-1851.[doi:doi:10.3969/j.issn.1000-4440.2025.09.019]
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肠道高黏附性噬菌体对小鼠沙门氏菌感染的预防作用()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1844-1851
栏目:
加工贮藏·质量安全
出版日期:
2025-09-30

文章信息/Info

Title:
The preventive effect of a gut-adherent bacteriophage against Salmonella infection in mice
作者:
纪林春1; 李悦2; 包红朵2; 卢河东1; 王冉2
(1.淮阴工学院生命科学与食品工程学院,江苏淮安223001；2.江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014)
Author(s):
JI Linchun1; LI Yue2; BAO Hongduo2; LU Hedong1; WANG Ran2
(1.School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223001, China;2.Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
高黏附性噬菌体; 肠道健康; 感染模型; 沙门氏菌; 小鼠
Keywords:
adherent bacteriophages; intestinal health; infection model; Salmonella; mice
分类号:
Q939.48
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.019
文献标志码:
A
摘要:
为研究高黏附性噬菌体对小鼠沙门氏菌感染的预防作用,本研究筛选到1株高黏附性噬菌体vB_SpuM_PA1-27。通过建立小鼠沙门氏菌感染模型，设置空白对照组、噬菌体对照组、沙门氏菌感染组和噬菌体预防组研究高黏附性噬菌体vB_SpuM_PA1-27对预防小鼠沙门氏菌感染的作用。结果显示,噬菌体预防组小鼠各肠段中均能检测到噬菌体分布,其中结肠中丰度最高,小鼠粪便中沙门氏菌载量显著降低(P＜0.05),未出现小鼠死亡。与沙门氏菌感染组比较,噬菌体预处理显著降低了小鼠脾脏指数(P＜0.05)以及结肠TNF-α、IL-1β mRNA表达水平(P＜0.05),显著提高了空肠绒毛长度(P＜0.05)、结肠长度(P＜0.05)和MUC2 mRNA表达水平(P＜0.05)。结果表明,高黏附性噬菌体能减轻沙门氏菌感染对小鼠的危害程度,保护小鼠肠道健康、减少小鼠死亡。本研究结果为高黏附性噬菌体在预防动物细菌感染方面的应用提供了重要数据参考。
Abstract:
In order to study the preventive effect of adherent bacteriophages against Salmonella infection in mice, a gut-adherent bacteriophage vB_SpuM_PA1-27 was isolated. By establishing a murine Salmonella infection model, this study investigated the preventive effect of vB_SpuM_PA1-27 against Salmonella infection through four experimental groups: blank control, bacteriophage control, Salmonella-infected group, and bacteriophage prevention group. The results demonstrated that in the bacteriophage prevention group, vB_SpuM_PA1-27 was detectable throughout all intestinal segments, with the highest abundance in colon. Concurrently, Salmonella loads in fecal samples were significantly reduced (P<0.05), and no mortality occurred in mice. Compared with the Salmonella-infected group, phage pretreatment significantly reduced the spleen index (P<0.05) and mRNA expression levels of TNF-α and IL-1β in colon (P<0.05), and significantly increased jejunal villus length (P<0.05), colon length (P<0.05), and MUC2 mRNA expression (P<0.05). The results indicated that vB_SpuM_PA1-27 could mitigate Salmonella-induced pathogenic impacts in mice, preserve intestinal health and reduce mortality. These findings can provide an important data reference for the application of adherent bacteriophages in the prevention of bacterial infection in animals.

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备注/Memo

备注/Memo:
收稿日期：2024-12-07基金项目：江苏省农业科技自主创新基金项目[CX(24)1015]；国家自然科学基金面上项目
[32273095]作者简介：纪林春(1998-),男,安徽淮南人,硕士研究生,研究方向为噬菌体。(E-mail)335099984@qq.com通讯作者：卢河东,(E-mail)luhd100@163.com；王冉,(E-mail)ranwang@jaas.ac.cn
更新日期/Last Update: 2025-10-27