[1]张鹏,吉星,孙利厂,等.江苏省南通地区虾源弧菌的分子流行病学分析[J].江苏农业学报,2025,(09):1791-1802.[doi:doi:10.3969/j.issn.1000-4440.2025.09.014]
　ZHANG Peng,JI Xing,SUN Lichang,et al.Molecular epidemiological analysis of Vibrio from shrimp in Nantong, Jiangsu province, China[J].,2025,(09):1791-1802.[doi:doi:10.3969/j.issn.1000-4440.2025.09.014]
点击复制

江苏省南通地区虾源弧菌的分子流行病学分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1791-1802
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-09-30

文章信息/Info

Title:
Molecular epidemiological analysis of Vibrio from shrimp in Nantong, Jiangsu province, China
作者:
张鹏1; 2; 吉星2; 孙利厂2; 朱树娇2; 何涛2; 王冉2
(1.江苏大学食品与生物工程学院,江苏镇江212013；2.江苏省农业科学院农产品质量安全与营养研究所/省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室,江苏南京210014）
Author(s):
ZHANG Peng1; 2; JI Xing2; SUN Lichang2; ZHU Shujiao2; HE Tao2; WANG Ran2
(1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;2.Institute of Food, Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China)
关键词:
弧菌; 虾; 耐药基因; 毒力基因; 进化分型
Keywords:
Vibrio; shrimp; drug resistance genes; virulence genes; evolutionary typing
分类号:
S945.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.014
文献标志码:
A
摘要:
为探明南通地区虾源弧菌的分子流行病学特征,本研究对在南通地区规模化虾养殖场中采集的560份病虾样本进行弧菌分离和鉴定,使用微量肉汤稀释法对分离株进行抗菌药物敏感性测试,基于全基因组测序技术分析分离株的耐药基因、毒力基因携带情况,并通过多位点序列分型(MLST)和系统发育树分析南通地区虾源弧菌的流行病学特征。结果表明,560份病虾样本中共分离出119株弧菌,检出率为21.25%,其中,溶藻弧菌为优势亚型,占比达73.95%。弧菌分离株对氨苄西林、头孢氨苄和多西环素的耐药率较高,分别为73.11%、58.82%、45.38%。弧菌分离株中共检测到47种耐药基因,其中tet(34)、tet(35)、blaampC检出率较高,分别为97.48%、88.24%、61.34%。分离株平均携带33个毒力基因,携带可编码细胞毒性的关键外毒素基因hlyA和rtxA的分离株分别为1株和7株。分离株ST型具有多样性,其中ST388为相对流行的ST型。溶藻弧菌分离株划分为1个进化分支,不同来源菌株之间的遗传进化关系较近；分离株85和分离株4与人类医疗临床来源的溶藻弧菌菌株有着高度同源性。本研究结果可为南通地区水产弧菌的有效防治提供科学依据。
Abstract:
To elucidate the molecular epidemiological characteristics of shrimp-derived Vibrios in Nantong area, Vibrio strains isolated from 560 diseased shrimp samples collected from large-scale shrimp farms in Nantong area were identified, and the antimicrobial susceptibility of the isolates was tested by micro-broth dilution method. Based on the whole genome sequencing technology, the drug resistance genes and virulence genes of the isolates were analyzed, and the epidemiological characteristics of shrimp-derived Vibrios in Nantong area were analyzed by multilocus sequence typing (MLST) and phylogenetic tree. The results showed that 119 strains of Vibrio were isolated from 560 diseased shrimp samples, with a detection rate of 21.25%. Among them, Vibrio alginolyticus was the dominant subtype, accounting for 73.95%. The resistance rates of the isolates to ampicillin, cephalexin and doxycycline were 73.11%, 58.82% and 45.38%, respectively. A total of 47 drug resistance genes were detected in the isolates, among which tet(34), tet(35) and blaampC had higher detection rates of 97.48%, 88.24% and 61.34%, respectively. The isolates carried an average of 33 virulence genes, and the numbers of isolates carrying the key cytotoxin-encoding genes hlyA and rtxA were one and seven, respectively. The ST types of the isolates were diverse, and ST388 was a relatively popular ST type. The isolates of V. alginolyticus were divided into one evolutionary branch, and the genetic evolution relationship between the strains from different sources was close. The isolate 85 and isolate 4 were highly homologous to the V. alginolyticus strains from human clinical sources. The results of this study can provide a scientific basis for the effective prevention and control of Vibrio infections in aquaculture in Nantong area.

参考文献/References:

[1]郑志伟. 食品中弧菌的耐药性和致病性研究[D]. 杨凌：西北农林科技大学,2019.
[2]THOMPSON F L, IIDA T, SWINGS J. Biodiversity of vibrios[J]. Microbiology and Molecular Biology Reviews,2004,68(3):403-431.
[3]YE L W, ZHENG Z W, WANG Y L, et al. Integrating metagenomic and isolation strategies revealed high contamination of pathogenies and resistome in market shrimps[J]. Science of the Total Environment,2024,926:171924.
[4]郑玉东, 张翔, 姚梦丽, 等. 养殖环境及贝源溶藻弧菌MLST分型及其毒力基因、耐药性分析[J]. 渔业科学进展, 2024, 45(1): 211-223.
[5]谢腾飞. 中国食源性副溶血性弧菌遗传多样性分析与冷胁迫研究[D]. 广州：华南理工大学,2018.
[6]ALLARD M W. The future of whole-genome sequencing for public health and the clinic[J]. Journal of Clinical Microbiology,2016,54(8):1946-1948.
[7]沈应博,史晓敏,沈建忠,等. 全基因组测序与生物信息学分析在细菌耐药性研究中的应用[J]. 生物工程学报,2019,35(4):541-557.
[8]BERTELLI C, GREUB G. Rapid bacterial genome sequencing:methods and applications in clinical microbiology[J]. Clinical Microbiology and Infection,2013,19(9):803-813.
[9]吴书香,李丽倩,姚琳,等. 海产源多重耐药共生菌的耐药表型及耐药基因分析[J]. 食品安全质量检测学报,2023,14(2):248-255.
[10]刘军军. 上海市水产品及其养殖环境中副溶血性弧菌和霍乱弧菌的流行、毒力及抗生素耐药性分析[D]. 上海：上海海洋大学,2022.
[11]安伟,张海强,高晓华. 上海地区虾源弧菌分离鉴定及分子分型研究[J]. 中国农学通报,2018,34(36):140-145.
[12]吴俊敏,王艺,郝婧薇,等. 湛江地区患病对虾池塘中副溶血性弧菌的MLST分型和新型耐药毒株的分离鉴定[J]. 大连海洋大学学报,2023,38(1):59-67.
[13]高晓华,安伟,张明辉. 上海凡纳滨对虾源副溶血弧菌耐药性及其耐药基因检测分析[J]. 南方农业学报,2021,52(3):827-836.
[14]魏文娟,赵姝,王元,等. 副溶血弧菌养殖对虾分离株耐药性及耐药基因分析[J]. 南方水产科学,2020,16(1):9-16.
[15]李璐璐,骆延波,齐静,等. CLSI药物敏感性试验标准VET01-A4概述及更新要点[J]. 中国家禽,2014,36(24):63-66,69.
[16]吉星,李俊,王冉,等. 金黄色葡萄球菌毒力调控及减毒药物研究进展[J]. 畜牧兽医学报,2025,56(4):1594-1607.
[17]LETUNIC I, BORK P. Interactive tree of life (iTOL) v5:an online tool for phylogenetic tree display and annotation[J]. Nucleic Acids Research,2021,49(W1):W293-W296.
[18]KOTLOWSKI R, GRECKA K, KOT B, et al. New approaches for Escherichia coli genotyping[J]. Pathogens,2020,9(2):73.
[19]CHOI G, CHOI S H. Complex regulatory networks of virulence factors in Vibrio vulnificus[J]. Trends in Microbiology,2022,30(12):1205-1216.
[20]BENITEZ J A, SILVA A J. Vibrio cholerae hemagglutinin(HA)/protease:an extracellular metalloprotease with multiple pathogenic activities[J]. Toxicon,2016,115:55-62.
[21]KWAK J S, JEONG H G, SATCHELL K J F. Vibrio vulnificus rtxA1 gene recombination generates toxin variants with altered potency during intestinal infection[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(4):1645-1650.
[22]LEE K K, YU S R, YANG T I, et al. Isolation and characterization of Vibrio alginolyticus isolated from diseased kuruma prawn,Penaeus japonicus[J]. Letters in Applied Microbiology,1996,22(2):111-114.
[23]林燕,郑双来. 一起由溶藻弧菌引起食源性疾病疫情的调查分析[J]. 中国农村卫生事业管理,2017,37(1):59-60.
[24]程亚琪,隋凌云. 一起溶藻弧菌引起的食物中毒[J]. 临床医药文献电子杂志,2014,1(11):2043.
[25]LEE D Y, MOON S Y, LEE S O, et al. Septic shock due to Vibrio alginolyticus in a cirrhotic patient:the first case in Korea[J]. Yonsei Medical Journal,2008,49(2):329-332.
[26]王筱,梁艳茹,周雨晴,等. 2017-2021年上海市浦东新区水产品中致病性弧菌监测分析[J]. 中国卫生检验杂志,2023,33(12):1437-1440.
[27]谭维维,鞠佩锋,许海燕. 2017-2022年南通市副溶血性弧菌污染状况分析[J]. 中国卫生检验杂志,2023,33(13):1566-1568,1572.
[28]秦思,乔昕,沈赟,等. 2016年江苏省淡水养殖环节常见致病性弧菌污染状况调查[J]. 食品安全质量检测学报,2019,10(12):3946-3951.
[29]WATTS J E M, SCHREIER H J, LANSKA L, et al. The rising tide of antimicrobial resistance in aquaculture:sources,sinks and solutions[J]. Marine Drugs,2017,15(6):158.
[30] LIU J, WU Q, XU H, et al. Change of antibiotic resistance in Vibrio spp. during shrimp culture in Shanghai[J]. Aquaculture,2024,580:740303.
[31]王娟,刘秀峰,陈凡冰,等. 福建省福清市2021-2023年副溶血性弧菌食源性疾病暴发分离株病原学特征与溯源分析[J]. 中国热带医学,2024,24(6):740-744.
[32]董慧明,种婷,刘霓,等. 辽宁省市售水产品中副溶血性弧菌的污染状况及耐药性分析[J]. 食品安全质量检测学报,2021,12(12):5066-5071.
[33]黄妙芬,刘坚基,连宪强,等. 2021-2022年广东省深圳市大鹏新区海水及海产品溶藻弧菌的污染状况、毒力基因分布和耐药性研究[J]. 疾病监测,2024,39(8):1074-1078.
[34]邓佩韦,郑双芝,严晗, 等. 杭州市水产品中溶藻弧菌耐药表型及耐药基因分布特征[J]. 食品与发酵工业,2025,51(9):319-326.
[35]吕蓓,吴龙飞,张英英,等. 132株副溶血性弧菌毒力基因及耐药性分析[J]. 中国卫生检验杂志,2020,30(8):941-943.
[36]郑宏源,闫琳,杨超,等. 溶藻弧菌群体基因组学研究[J]. 遗传,2021,43(4):350-361.
[37]YE L W, ZHENG Z W, XUY T, et al. Prevalence and genetic basis of tetracycline resistance in Vibrio parahaemolyticus isolates recovered from food products in Shenzhen,China during 2013 to 2021[J]. Science of the Total Environment,2023,902:166026.
[38]SHANTHI AMUDHAN M, SEKAR U, KAMALANATHAN A, et al. Bla(IMP) and bla(VIM) mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India[J]. Journal of Infection in Developing Countries,2012,6(11):757-762.
[39]时晨,于良,王悦,等. 秦皇岛市一起由副溶血性弧菌引起的食源性疾病暴发事件的检测和溯源分析[J]. 食品安全质量检测学报,2021,12(9):3503-3507.
[40]李翠苹,翟倩倩,王想,等. 对虾养殖池副溶血弧菌的分离鉴定及其耐药特征、毒力基因分析[J]. 渔业科学进展,2020,41(6):174-180.
[41]张婷,杨梦华. 副溶血弧菌的毒力基因表达调控的分子机制[J]. 微生物学报,2020,60(7):1345-1357.
[42]廖冬冬,曹祥玉,林馨,等. 福州市2016-2023年水产品副溶血性弧菌污染状况及毒力基因检测[J]. 海峡预防医学杂志,2024,30(1):60-62.
[43]李宏杰,陈志杭,叶斌,等. 食源性相关腹泻患者粪副溶血性弧菌检测结果分析[J]. 中国卫生检验杂志,2020,30(2):154-157.

相似文献/References:

[1]林婷,杨胜平,谢晶,等.虾黑变形成机制及其抑制方法研究进展[J].江苏农业学报,2020,(06):1605.[doi:doi:10.3969/j.issn.1000-4440.2020.06.034]
　LIN Ting,YANG Sheng-ping,XIE Jing,et al.Research progress on mechanisms of melanosis development and anti-melanosis methods in shrimps[J].,2020,(09):1605.[doi:doi:10.3969/j.issn.1000-4440.2020.06.034]

备注/Memo

备注/Memo:
收稿日期:2024-12-11基金项目:“十四五”国家重点研发计划课题(2023YFD1800303) 作者简介:张鹏(1997-),男,山东泰安人,硕士研究生,主要从事细菌与噬菌体相互作用研究。(E-mail)zpeng1120805610@163.com 通讯作者:何涛,(E-mail)vethetao@163.com；王冉,(E-mail)ranwang@jaas.ac.cn
更新日期/Last Update: 2025-10-27