[1]车阳,邢志鹏,蒋伟勤,等.外源植物生长调节剂对高温胁迫下水稻颖花分化及退化的影响[J].江苏农业学报,2025,(08):1475-1483.[doi:doi:10.3969/j.issn.1000-4440.2025.08.003]
　CHE Yang,XING Zhipeng,JIANG Weiqin,et al.Effects of exogenous plant growth regulators on spikelet differentiation and degeneration in rice under high temperature stress conditions[J].,2025,(08):1475-1483.[doi:doi:10.3969/j.issn.1000-4440.2025.08.003]
点击复制

外源植物生长调节剂对高温胁迫下水稻颖花分化及退化的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1475-1483
栏目:
遗传育种·生理生化
出版日期:
2025-08-31

文章信息/Info

Title:
Effects of exogenous plant growth regulators on spikelet differentiation and degeneration in rice under high temperature stress conditions
作者:
车阳1; 邢志鹏2; 蒋伟勤1; 李可1; 宋佳敏1; 何佳伟1; 李其胜1; 文廷刚1; 杜小凤1; 杨文飞1
(1.江苏徐淮地区淮阴农业科学研究所,江苏淮安223001；2.扬州大学水稻产业工程技术研究院,江苏扬州225009；3.淮阴师范学院,江苏淮安223300)
Author(s):
CHE Yang1; XING Zhipeng2; JIANG Weiqin1; LI Ke1; SONG Jiamin1; HE Jiawei1; LI Qisheng1; WEN Tinggang1; DU Xiaofeng1; YANG Wenfei1
(1.Huaiyin Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Huai’an 223001, China;2.Research Institute of Rice Industrial Engineering Technology, Yangzhou University, Yangzhou 225009, China;3.Huaiyin Normal University, Huai’an 223300, China)
关键词:
水稻; 高温胁迫; 植物生长调节剂; 颖花分化、颖花退化; 产量
Keywords:
rice; high temperature stress; plant growth regulators; spikelet differentiation; spikelet degeneration; yield
分类号:
S511；S162.5
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.003
文献标志码:
A
摘要:
水稻穗分化期是产量形成的关键期,此期若遇极端高温会严重威胁颖花发育和产量稳定。本研究利用自然高温天气,以水稻品种南粳9108和宁香粳9号为材料,在幼穗分化期2次喷施S-诱抗素(ABA)、2,4-表油菜素内酯(BL)和茉莉酸甲酯(MeJA),并设置清水喷施为对照(CK),研究外源植物生长调节剂对水稻颖花分化与退化以及产量构成特征的影响。结果表明,在穗分化期遭遇高温的情况下,与对照相比,ABA处理、BL处理、MeJA处理南粳9108每穗颖花分化数、每穗颖花现存率、着粒密度均显著提高(P＜0.05),每穗颖花退化数显著降低(P＜0.05)；与对照相比,ABA处理和MeJA处理宁香粳9号每穗颖花分化数、每穗颖花现存率、着粒密度显著提升(P＜0.05),每穗颖花退化数显著下降(P＜0.05)；喷施植物生长调节剂优化了水稻颖花在一次枝梗和二次枝梗上的分布,与对照相比,二次枝梗颖花数增幅达20.42%～44.47%,二次枝梗颖花现存率显著提升(P＜0.05)。通径分析结果显示,水稻每穗颖花分化数对产量的直接正向效应显著(P＜0.05),每穗颖花退化数负向效应极显著(P＜0.01),由此可知,植物生长调节剂通过“促分化-抑退化”双重途径改善水稻穗部结构。产量构成因素方面,植物生长调节剂主要通过提高结实率和每穗粒数实现增产。本研究结果为水稻抗逆丰产栽培提供了重要技术路径。
Abstract:
The panicle differentiation stage of rice is a critical period for yield formation. Extreme high temperatures during this stage can severely threaten spikelet development and yield stability. In this study, utilizing natural high-temperature conditions, rice varieties Nanjing 9108 and Ningxiangjing 9 were used as materials. During the young panicle differentiation stage, exogenous plant growth regulators, namely S-abscisic acid (ABA), 2,4-epibrassinolide (BL), and methyl jasmonate (MeJA), were sprayed twice, with clear water spraying set as the control (CK). The effects of exogenous plant growth regulators on rice spikelet differentiation, degeneration, and yield component characteristics were investigated. The results showed that under high-temperature conditions during the panicle differentiation stage, compared with the control, ABA, BL, and MeJA treatments significantly increased the number of differentiated spikelets per panicle, spikelet survival rate per panicle, and the grain density of Nanjing 9108 (P＜0.05), while significantly reducing the number of degenerated spikelets per panicle (P＜0.05). For Ningxiangjing 9, compared with the control, ABA and MeJA treatments significantly increased the number of differentiated spikelets per panicle, spikelet survival rate per panicle, and the grain density (P＜0.05), and significantly decreased the number of degenerated spikelets per panicle (P＜0.05). Spraying plant growth regulators optimized the distribution of rice spikelets on primary and secondary branches. Compared with the control, the number of spikelets on secondary branches increased by 20.42%-44.47%, and the survival rate of spikelets on secondary branches was significantly improved (P＜0.05). Path analysis results showed that the number of differentiated spikelets per panicle had a significant direct positive effect on yield (P＜0.05), while the number of degenerated spikelets per panicle had an extremely significant negative effect (P＜0.01). It can be concluded that plant growth regulators improve the rice panicle structure through a dual pathway of promoting differentiation and inhibiting degradation. In terms of yield components, plant growth regulators significantly increased yield by improving the seed setting rate and the number of grains per panicle. The results of this study can provide an important technical approach for stress-resistant and high-yield rice cultivation.

参考文献/References:

[1]张洪程,胡雅杰,杨建昌,等. 中国特色水稻栽培学发展与展望[J]. 中国农业科学,2021,54(7):1301-1321.
[2]李勇,杨晓光,叶清,等. 全球气候变暖对中国种植制度可能影响 Ⅸ. 长江中下游地区单双季稻高低温灾害风险及其产量影响[J]. 中国农业科学,2013,46(19):3997-4006.
[3]CHEN C Q, VAN GROENIGEN K J, YANG H Y, et al. Global warming and shifts in cropping systems together reduce China’s rice production[J]. Global Food Security,2020,24:100359.
[4]林纾,李红英,黄鹏程,等. 2022年夏季我国高温干旱特征及其环流形势分析[J]. 干旱气象,2022,40(5):748-763.
[5]段骅,佟卉,刘燕清,等. 高温和干旱对水稻的影响及其机制的研究进展[J]. 中国水稻科学,2019,33(3):206-218.
[6]徐敏,徐经纬,徐萌,等. 水稻高温热害强度时空变化特征与预报模型构建[J]. 农业工程学报,2024,40(10):97-106.
[7]PENG S B, HUANG J L, SHEEHY J E, et al. Rice yields decline with higher night temperature from global warming[J]. Proceedings of the National Academy of Sciences of the United States of America,2004,101(27):9971-9975.
[8]ZHANG C X, LI G Y, CHEN T T, et al. Heat stress induces spikelet sterility in rice at anthesis through inhibition of pollen tube elongation interfering with auxin homeostasis in pollinated pistils[J]. Rice,2018,11(1):14.
[9]WU C, CUI K H, TANG S, et al. Intensified pollination and fertilization ameliorate heat injury in rice (Oryza sativa L.) during the flowering stage[J]. Field Crops Research,2020,252:107795.
[10]WANG W C, CUI K H, HU Q Q, et al. Response of spikelet water status to high temperature and its relationship with heat tolerance in rice[J]. The Crop Journal,2021,9(6):1344-1356.
[11]CAO Y Y, CHEN Y H, CHEN M X, et al. Growth characteristics and endosperm structure of superior and inferior spikelets of indica rice under high-temperature stress[J]. Biologia Plantarum,2016,60(3):532-542.
[12]WU C, CUI K H, WANG W C, et al. Heat-induced phytohormone changes are associated with disrupted early reproductive development and reduced yield in rice[J]. Scientific Reports,2016,6:34978.
[13]TANG S, ZHAO Y F, RAN X, et al. Exogenous application of methyl jasmonate at the booting stage improves rice’s heat tolerance by enhancing antioxidant and photosynthetic activities[J]. Agronomy,2022,12(7):1573.
[14]王强,陈雷,张晓丽,等. 化学调控对水稻高温热害的缓解作用研究[J]. 中国稻米,2015,21(4):80-82.
[15]陈燕华,王亚梁,陈惠哲,等. 油菜素甾醇类化合物对水稻抗逆的作用及其机制研究进展[J]. 作物研究,2020,34(6):597-604.
[16]凌启鸿,蔡建中,苏祖芳. 叶龄余数在稻穗分化进程鉴定中的应用价值[J]. 中国农业科学,1980,13(4):1-11.
[17]曹云英. 高温对水稻产量与品质的影响及其生理机制[D]. 扬州:扬州大学,2009.
[18]陈燕华,王亚梁,朱德峰,等. 外源油菜素内酯缓解水稻穗分化期高温伤害的机理研究[J]. 中国水稻科学,2019,33(5):457-466.
[19]姬东玲. 穗分化期高温胁迫下茉莉酸甲酯对水稻穗形态建成的调控机理[D]. 扬州:扬州大学,2024.
[20]松岛省三. 稻作的理论与技术[M]. 北京:中国农业出版社,1966.
[21]马义虎,杨祥田. 高温胁迫对水稻的影响及其对策的研究进展[J]. 中国农学通报,2015,31(9):1-8.
[22]曹云英,段骅,杨立年,等. 减数分裂期高温胁迫对耐热性不同水稻品种产量的影响及其生理原因[J]. 作物学报,2008,34(12):2134-2142.
[23]王亚梁,张玉屏,曾研华,等. 水稻穗分化期高温对颖花分化及退化的影响[J]. 中国农业气象,2015,36(6):724-731.
[24]刘霞. 外源茉莉酸甲酯和亚精胺对水稻高温胁迫的缓解效应[D]. 南京:南京农业大学,2016.
[25]姜辉,姜树坤,陈丽丽,等. 水稻顶端颖花退化的形态与发育学研究[J]. 黑龙江农业科学,2016(1):7-11.
[26]WANG Z Q, ZHANG W Y, YANG J C. Physiological mechanism underlying spikelet degeneration in rice[J]. Journal of Integrative Agriculture,2018,17(7):1475-1481.
[27]符冠富,张彩霞,杨雪芹,等. 水杨酸减轻高温抑制水稻颖花分化的作用机理研究[J]. 中国水稻科学,2015,29(6):637-647.
[28]文廷刚,王伟中,杨文飞,等. 水稻穗分化期外源植物生长调节剂处理对颖花分化与退化的影响[J]. 江苏农业学报,2019,35(3):514-522.

相似文献/References:

[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(08):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
[2]王在满,郑乐,张明华,等.不同播种方式对直播水稻倒伏指数和根系生长的影响[J].江苏农业学报,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
　WANG Zai-man,ZHENG Le,ZHANG Ming-hua,et al.Effects of seeding manners on lodging index and root growth of directseeded rice[J].,2016,(08):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
[3]易能,薛延丰,石志琦,等.微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用[J].江苏农业学报,2016,(04):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
　YI Neng,XUE Yan-feng,SHI Zhi-qi,et al.Inhibitory effect of microcystins on seed germination and seedling growth of rice[J].,2016,(08):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
[4]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(08):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
[5]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(08):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[6]孙玲,单捷,毛良君,等.基于遥感和Moran's I指数的水稻面积变化空间自相关性研究[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
　SUN Ling,SHAN Jie,MAO Liang-jun,et al.Spatial autocorrelation of changes in paddy rice area based on remote sensing and Moran’s I index[J].,2016,(08):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
[7]张晓忆,李卫国,景元书,等.多种光谱指标构建决策树的水稻种植面积提取[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
　ZHANG Xiao-yi,LI Wei-guo,JING Yuan-shu,et al.Extraction of paddy rice area by constructing the decision tree with multiple spectral indices[J].,2016,(08):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
[8]裔传灯,李玮,王德荣,等.水稻GW5基因的1212-bp Indel变异对粒形的影响[J].江苏农业学报,2016,(06):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
　YI Chuan-deng,LI Wei,WANG De-rong,et al.Effect of 1212-bp Indel variation of gene GW5 on rice grain shape[J].,2016,(08):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
[9]刘红江,陈虞雯,张岳芳,等.不同播栽方式对水稻叶片光合特性及产量的影响[J].江苏农业学报,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
　LIU Hong-jiang,CHEN Yu-wen,ZHANG Yue-fang,et al.Effects of planting pattern on leaf photosynthetic characteristics and yield of rice[J].,2016,(08):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
[10]郭保卫,许轲,魏海燕,等.钵苗机插水稻茎秆的抗倒伏能力[J].江苏农业学报,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
　GUO Bao-wei,XU Ke,WEI Hai-yan,et al.Culm lodging resistance characteristics of bowl seedling mechanical-transplanting rice[J].,2016,(08):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
[11]张斌,杨昕霞,袁志辉.水稻响应热胁迫核心基因的筛选与鉴定[J].江苏农业学报,2021,(04):817.[doi:doi:10.3969/j.issn.1000-4440.2021.04.001]
　ZHANG Bin,YANG Xin-xia,YUAN Zhi-hui.Screening and identification of core genes responding to heat stress in rice[J].,2021,(08):817.[doi:doi:10.3969/j.issn.1000-4440.2021.04.001]
[12]徐鹏,贺一哲,尤翠翠,等.高温胁迫导致水稻颖花败育的机理及其防御措施研究进展[J].江苏农业学报,2023,(01):255.[doi:doi:10.3969/j.issn.1000-4440.2023.01.029]
　XU Peng,HE Yi-zhe,YOU Cui-cui,et al.Research progress on the mechanism and defense measures of rice spikelet abortion caused by high temperature stress[J].,2023,(08):255.[doi:doi:10.3969/j.issn.1000-4440.2023.01.029]

备注/Memo

备注/Memo:
收稿日期：2025-05-13基金项目：淮安市科技计划项目(HAS202405)；农业重大技术协同推广计划项目(2024-ZYXT-07)；淮安市农业科学研究院科研发展基金项目(HNY202114)；亚夫科技服务项目[KF(24)1217]作者简介：车阳(1996-),男,山东菏泽人,硕士,助理研究员,研究方向为植物逆境生理与调控技术。(E-mail)cheyang@jaas.ac.cn通讯作者：顾大路,(E-mail)1302680742@qq.com；章安康,(E-mail)2530326440@qq.com
更新日期/Last Update: 2025-09-23