[1]赵霈泽,万文涛,赵仁慧,等.基于CRISPR/Cas9基因编辑技术改良扬麦18农艺与品质性状[J].江苏农业学报,2025,(08):1466-1474.[doi:doi:10.3969/j.issn.1000-4440.2025.08.002]
　ZHAO Peize,WAN Wentao,ZHAO Renhui,et al.Improvement of agronomic and quality traits of Yangmai 18 using CRISPR/Cas9 gene editing technology[J].,2025,(08):1466-1474.[doi:doi:10.3969/j.issn.1000-4440.2025.08.002]
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基于CRISPR/Cas9基因编辑技术改良扬麦18农艺与品质性状()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1466-1474
栏目:
遗传育种·生理生化
出版日期:
2025-08-31

文章信息/Info

Title:
Improvement of agronomic and quality traits of Yangmai 18 using CRISPR/Cas9 gene editing technology
作者:
赵霈泽1; 2; 万文涛2; 赵仁慧2; 蒋正宁2; 吴荣林2; 别同德2; 陈升位1
(1.云南农业大学农业与生物技术学院,云南昆明650000；2.农业农村部长江中下游小麦生物学与遗传育种重点实验室/江苏里下河地区农业科学研究所,江苏扬州225007)
Author(s):
ZHAO Peize1; 2; WAN Wentao2; ZHAO Renhui2; JIANG Zhengning2; WU Ronglin2; BIE Tongde2; CHEN Shengwei1
(1.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650000, China;2.Key Laboratory of Wheat Biology and Genetic Improvement for Lower & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China)
关键词:
基因编辑; 春化基因; 籽粒硬度基因; 种皮颜色基因
Keywords:
gene editing; vernalization gene; grain hardness gene; seed coat color gene
分类号:
S512.103
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.002
文献标志码:
A
摘要:
江苏省是中国重要的小麦主产省份,地跨长江中下游和黄淮两大小麦主产区。长江中下游麦区种植的小麦以红皮春性小麦为主,一般穗形大,株型松散,对赤霉病、白粉病和穗发芽等有较好抗性。黄淮麦区种植的小麦以白皮冬性小麦为主,表现为株型紧凑、穗数多,但大多数对赤霉病、白粉病和穗发芽敏感。为创制满足不同应用需求的小麦新种质,以红皮春性软质品种扬麦18为材料,利用CRISPR/Cas9技术对春化相关基因Vrn-D1、籽粒硬度相关基因Pina和Pinb及种皮颜色相关基因Myb10进行编辑,共获得6种Vrn-D1突变体、5种Pin突变体及7种Myb10突变体。表型鉴定结果表明,与野生型相比,Vrn-D1突变体第2、第4节间长度显著降低（除vrnM5编辑系）,Pin突变体籽粒硬度显著增加，5个Myb10突变体种皮的颜色变为白色。本研究结果为利用CRISPR/Cas9基因编辑技术改良小麦特定农艺性状和品质性状提供了参考。
Abstract:
Jiangsu province is an important wheat-producing province in China, spanning the middle and lower reaches of the Yangtze River (MLRYR) and the Huang-Huai region, two major wheat-growing areas. The wheat planted in the MLRYR is mainly red-skinned spring wheat, which usually has large panicles and a loose plant type, and is resistant to Fusarium head blight (FHB), powdery mildew (PM), and pre-harvest sprouting (PHS). In contrast, the wheat planted in the Huang-Huai region is mainly white-skinned winter wheat, which has a compact plant type and a higher number of panicles, but is generally susceptible to FHB, PM, and PHS. To develop new wheat germplasms that meet different application needs, the vernalization-related gene Vrn-D1, grain hardness-related genes Pina and Pinb, and the seed coat color-related gene Myb10 were edited in the red-skinned spring soft wheat variety Yangmai 18 using CRISPR/Cas9 technology. A total of six Vrn-D1 mutants, five Pin mutants, and seven Myb10 mutants were obtained. Phenotypic identification results showed that compared with the wild type, the second and fourth internode lengths of the Vrn-D1 mutants were significantly reduced (with the exception of the vrnM5 line), the grain hardness of the Pin mutants was significantly increased, and the seed coat color of five Myb10 mutants changed from red to white. The results of this study provide a reference for using CRISPR/Cas9 gene editing technology to improve specific agronomic and quality traits of wheat.

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备注/Memo

备注/Memo:
收稿日期：2025-01-10基金项目：国家自然科学基金项目(32472102)；江苏里下河地区农业科学研究所科研专项[SJ(22)114]作者简介：赵霈泽(2000-),男,江苏无锡人,硕士研究生,主要从事小麦遗传育种研究。(E-mail)m13771257610@163.com 通讯作者：陈升位,(E-mail) ynkmcsw@ynau.edu.cn
更新日期/Last Update: 2025-09-23