[1]林辉锋,许克正,周辉明,等.不同LED光照对兰属然洛组织培养苗生长及生理生化特性的影响[J].江苏农业学报,2025,(07):1302-1311.[doi:doi:10.3969/j.issn.1000-4440.2025.07.006]
　LIN Huifeng,XU Kezheng,ZHOU Huiming,et al.Effects of different LED illumination treatments on the growth and physicochemical properties of Cymbidium Ranluo tissue-cultured seedlings[J].,2025,(07):1302-1311.[doi:doi:10.3969/j.issn.1000-4440.2025.07.006]
点击复制

不同LED光照对兰属然洛组织培养苗生长及生理生化特性的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年07期
页码:
1302-1311
栏目:
遗传育种·生理生化
出版日期:
2025-07-31

文章信息/Info

Title:
Effects of different LED illumination treatments on the growth and physicochemical properties of Cymbidium Ranluo tissue-cultured seedlings
作者:
林辉锋1; 许克正1; 周辉明1; 甘玮欣1; 林发壮1; 张政斌1; 宋彩凤2
(1.三明市农业科学研究院花卉研究所,福建三明365051；2.三明市森彩生态农业发展有限公司,福建三明365051)
Author(s):
LIN Huifeng1; XU Kezheng1; ZHOU Huiming1; GAN Weixin1; LIN Fazhuang1; ZHANG Zhengbin1; SONG Caifeng2
(1.Flower Research Institute, Sanming Academy of Agricultural Sciences, Sanming 365051, China;2.Sanming Sencai Ecological Agriculture Development Co., Ltd., Sanming 365051, China)
关键词:
LED光照; 兰属然洛; 组织培养
Keywords:
LED illumination; Cymbidium Ranluo; tissue culture
分类号:
S682.31
DOI:
doi:10.3969/j.issn.1000-4440.2025.07.006
文献标志码:
A
摘要:
以兰属然洛组织培养根状茎和组织培养苗为试验材料,研究5种不同发光二极管(LED)光照[红光（R）、红光∶蓝光（B）=6∶4（R6B4）、红光∶蓝光=5∶5（R5B5）、红光∶蓝光=4∶6（R4B6）、蓝光]对兰属然洛根状茎增殖分化和组织培养苗壮苗生根阶段生长和生理生化性质的影响。结果表明,在根状茎增殖分化阶段,与荧光灯对照(CK)相比,红光∶蓝光=5∶5(R5B5)处理兰属然洛组织培养根状茎粗度显著增加、分化率最高；可溶性蛋白含量随蓝光比例的增加呈现先降低后上升的趋势,而可溶性糖含量则呈现先增加后降低趋势,R6B4处理最高；过氧化氢含量、活性氧含量随蓝光比例增加而减少,而过氧化氢酶活性随蓝光比例增加而增强,R5B5处理的过氧化氢含量与CK相比差异不显著,而活性氧含量和过氧化氢酶活性与CK相比显著增加。在壮苗生根阶段,R5B5处理的组织培养苗叶长、叶宽、假鳞茎宽、叶片数、株高、根长、根粗、地上部鲜重以及叶绿素a含量、叶绿素b含量、类胡萝卜素含量和总叶绿素含量较高；组织培养苗可溶性糖含量均随着红光比例的降低先升高后降低,与CK相比R5B5处理降低最小；R5B5处理CRY2基因的相对表达量最高。综上所述,不同LED光照处理对兰属然洛根状茎的增殖分化以及组织培养苗的生长、叶绿素含量、可溶性蛋白含量、可溶性糖含量、抗氧化系统、CRY2基因表达均有不同程度的影响,以R5B5处理的效果最好。
Abstract:
Using Cymbidium Ranluo tissue-cultured rhizomes and tissue-cultured seedlings as experimental materials, this study investigated the effects of five LED illumination treatments on rhizome proliferation/differentiation and seedling growth/physicochemical properties during the seedling-strengthening and rooting stages. The five treatments were red light (R), red light∶blue light = 6∶4 (R6B4), red light∶blue light = 5∶5 (R5B5), red light∶blue light = 4∶6 (R4B6), and blue light (B). The results showed that during the rhizome proliferation and differentiation stage, compared with the fluorescent lamp control (CK), the diameter of Cymbidium Ranluo tissue-cultured rhizomes increased significantly and the differentiation rate was the highest under R5B5 treatment. The soluble protein content decreased firstly and then increased with the increase of blue light ratio, while the soluble sugar content increased firstly and then decreased, which was the highest under R6B4 treatment. The content of hydrogen peroxide and reactive oxygen species decreased with the increase of blue light ratio, while the activity of catalase increased with the increase of blue light ratio. There was no significant difference in hydrogen peroxide content between the R5B5 treatment and the CK, while the reactive oxygen species content and catalase activity increased significantly compared to CK. During the seedling-strengthening and rooting stages, R5B5-treated tissue-cultured seedlings exhibited higher values in leaf length, leaf width, pseudobulb width, leaf number, plant height, root length, root diameter, fresh weight of aboveground parts, chlorophyll a content, chlorophyll b content, carotenoid content and total chlorophyll content. The soluble sugar content of the tissue-cultured seedlings first increased and then decreased with the reduction of the red light ratio, with the R5B5 treatment showing the smallest decrease compared to the CK. The relative expression of CRY2 gene was the highest under R5B5 treatment. In summary, different LED illumination treatments have varying effects on the rhizome proliferation and differentiation of Cymbidium Ranluo, as well as on the growth, chlorophyll content, soluble protein content, soluble sugar content, antioxidant system, and CRY2 gene expression of the tissue-cultured seedlings, with the R5B5 treatment showing the best results.

参考文献/References:

[1]王玲. 国兰育种技术研究进展[J]. 生物灾害科学,2022,45(1):8-13.
[2]贾思思,曾瑞珍,张志胜,等. 兰花分子育种技术研究进展[J]. 华南农业大学学报,2024,45(1):1-14.
[3]HINSLEY A, DE BOER H J, FAY M F, et al. A review of the trade in orchids and its implications for conservation[J]. Botanical Journal of the Linnean Society,2018,186(4):435-455.
[4]孙芳. 春兰蕙兰杂交育种技术研究[D]. 泰安:山东农业大学,2013.
[5]MACEDO A F, LEAL-COSTA M V, TAVARES E S, et al. The effect of light quality on leaf production and development of in vitro-cultured plants of Alternanthera brasiliana Kuntze[J]. Environmental and Experimental Botany,2011,70(1):43-50.
[6]IACONA C, MULEO R. Light quality affects in vitro adventitious rooting and ex vitro performance of cherry rootstock Colt[J]. Scientia Horticulturae,2010,125(4):630-636.
[7]李夏媛,王玉英,苏畅,等. LED不同光质对兰花‘霞光’组培苗生长及生理特性的影响[J]. 热带农业科学,2016,36(5):64-69.
[8]曾惠容. LED节能灯节能效果实证研究[J]. 安徽农学通报,2011,17(4):131-133.
[9]SHIN K S, MURTHY H N, HEO J W, et al.The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants[J]. Acta Physiologiae Plantarum,2008,30(3):339-343.
[10]HAHN E J, KOZAI T, PAEK K Y. Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro Growth of Rehmannia glutinosa plantlets[J]. Journal of Plant Biology,2000,43(4):247-250.
[11]KIM S J, HAHN E J, HEO J W, et al. Effects of LEDs on net photosynthetic rate,growth and leaf stomata of Chrysanthemum plantlets in vitro[J]. Scientia Horticulturae,2004,101(1/2):143-151.
[12]LI H M, XU Z G, TANG C M. Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro[J]. Plant Cell,Tissue and Organ Culture (PCTOC),2010,103(2):155-163.
[13]张金龙,赵红梅,瞿素萍,等. 不同LED光质对大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’F1代组培苗生长的影响[J]. 北方园艺,2024(10):61-68.
[14]刘洋,王玉英,朱雄,等. 不同LED光质对大花蕙兰与莲瓣兰杂交苗生长及生理生化特性的影响[J]. 西部林业科学,2020,49(5):30-36.
[15]杨贞,张永春,王艺程,等. LED光质对彩色马蹄莲组培苗生长及生理特性的影响[J]. 上海农业学报,2022,38(1):27-32.
[16]高晓芬,吴小梅,郝春磊,等. 基于红蓝光质配比的小花型蝴蝶兰组培苗生长调控[J]. 种子,2023,42(5):40-45.
[17]NHUT D T, TAKAMURA T, WATANABE H, et al. Sugar-free micropropagation of Eucalyptus citriodora using light-emitting diodes (LEDs) and film-rockwool culture system[J]. Environment Control in Biology,2002,40(2):147-155.
[18]SIMA Y H, YAO J M, HOU Y S, et al.Variations of hydrogen peroxide and catalase expression in Bombyx eggs during diapause initiation and termination[J]. Archives of Insect Biochemistry and Physiology,2011,77(2):72-80.
[19]景艳军,林荣呈. 蓝光受体CRY2化身“暗黑舞者”[J]. 植物学报,2024,59(6):878-882.
[20]张海良. 兰花芽分化的分子机理和相关功能基因克隆[D]. 广州:华南农业大学,2018.
[21]梁钧淞. 兰花根状茎苗分化的分子机理[D]. 广州:华南农业大学,2019.
[22]郑子松,王红,王神云,等. LED光源对结球甘蓝(Brassica oleracea var.Capitatal)不定芽再生的影响[J]. 天津农学院学报,2013,20(2):6-10.
[23]甘玮欣,曹奕鸯,陈玮婷,等. 光照对非洲菊‘明卉粉黛’组培苗生长的影响[J]. 亚热带农业研究,2023,19(4):261-267.
[24]肖波. 草坪草光合色素与抗性的关系研究进展[J]. 绿色科技,2016,18(9):154-155.
[25]ANZELIKA, RENATA, SILVA, et al. In vitro cultivation of grape culture under solid state lighting[J]. Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture,2007,26(3):235-245.
[26]郭莹,李海燕,阮氏月,等. LED不同光质对大花蕙兰‘爱神’×虎雪兰‘霞光’杂交组培苗叶绿素含量的影响[J]. 北方园艺,2015(18):77-80.
[27]周锦业,丁国昌,何荆洲,等. 不同光质对金线莲组培苗叶绿素含量及叶绿素荧光参数的影响[J]. 农学学报,2015,5(5):67-72.
[28]张微慧,张光伦. 光质对果树形态建成及果实品质的生理生态效应[J]. 中国农学通报,2007,23(1):78-83.
[29]邸秀茹,焦学磊,崔瑾,等. 新型光源LED辐射的不同光质配比光对菊花组培苗生长的影响[J]. 植物生理学通讯,2008,44(4):661-664.
[30]戴艳娇,王琼丽,张欢,等. 不同光谱的LEDs对蝴蝶兰组培苗生长的影响[J]. 江苏农业科学,2010,38(5):227-231.
[31]王淑杰,王家民,李亚东,等. 可溶性全蛋白、可溶性糖含量与葡萄抗寒性关系的研究[J]. 北方园艺,1996(2):77-78.
[32]林碧英,张瑜,林义章. 不同光质对豇豆幼苗光合特性和若干生理生化指标的影响[J]. 热带作物学报,2011,32(2):235-239.
[33]卢晓凤,骆隽言,韦明涵,等. 罗汉果甜苷与黄芪多糖联用对小鼠骨骼肌抗氧化能力的影响[J]. 南方农业学报,2024,55(7):1925-1934.
[34]侯君佑,单雅敬,孙彩勇,等. 孕穗期渍水对小麦花后根系形态、抗氧化酶活性及籽粒产量的影响[J]. 江苏农业学报,2024,40(3):385-393.
[35]税贤,陈甜甜,彭俊杰,等. 模拟增强UV-B辐射下台农一号芒果抗氧化响应机理[J]. 南方农业学报,2024,55(3):846-854.
[36]张雪莲,罗德旭,杨红,等. 外源褪黑素和硒对高温胁迫下辣椒生理特性和抗氧化系统的影响[J]. 江苏农业学报,2023,39(8):1729-1738.
[37]董颐玮,朱燕云,靳红梅. 生物纳米硒对杭白菜品质与抗氧化性能的影响[J]. 江苏农业学报,2023,39(2):479-488.
[38]申圣圣,杨利艳,乔美玉,等. 不同光质LED光源对鱼腥草幼苗膜脂过氧化及抗氧化酶的影响[J]. 中国瓜菜,2020,33(4):17-22.
[39]屈莹,谢久凤,王珂,等. 低温及光照对四季秋海棠叶片花色素苷合成的诱导及调控机理[J]. 河南农业大学学报,2018,52(3):342-349.
[40]RECZEK C R, CHANDEL N S. ROS-dependent signal transduction[J]. Current Opinion in Cell Biology,2015,33:8-13.
[41]LERCARI B, MOSCATELLI S, GHIRARDI E, et al. Photomorphogenic control of shoot regeneration from etiolated and light-grown hypocotyls of tomato[J]. Plant Science,1999,140(1):53-62.
[42]MULEO R, MORINI S. Light quality regulates shoot cluster growth and development of MM106 apple genotype in in vitro culture[J]. Scientia Horticulturae,2006,108(4):364-370.
[43]WANG W X, LU X D, LI L, et al. Photoexcited CRYPTOCHROME1 interacts with dephosphorylated BES1 to regulate brassinosteroid signaling and photomorphogenesis in Arabidopsis[J]. The Plant Cell,2018,30(9):1989-2005.
[44]叶宇芸. 隐花色素CRY1和CRY2在草莓生长发育中的功能鉴定[D]. 雅安:四川农业大学,2022.
[45]SHALITIN D, YANG H Y, MOCKLER T C, et al. Regulation of Arabidopsis cryptochrome 2 by blue-light-dependent phosphorylation[J]. Nature,2002,417(6890):763-767.
[46]王亚沉,包崇格,彭少丹,等. 光质对碧玉兰组培苗生长及若干生理指标的影响[J]. 南方农业学报,2013,44(5):740-744.

相似文献/References:

[1]樊小雪,凌丹丹,徐刚,等.不同LED光照对番茄果实品质和糖类物质含量的影响[J].江苏农业学报,2021,(04):944.[doi:doi:10.3969/j.issn.1000-4440.2021.04.017]
　FAN Xiao-xue,LING Dan-dan,XU Gang,et al.Effects of different LED light on fruit quality and sugar content of tomato[J].,2021,(07):944.[doi:doi:10.3969/j.issn.1000-4440.2021.04.017]
[2]郭峰,孙莹,安容慧,等.LED绿光处理对甘蓝采后品质及抗氧化活性的影响[J].江苏农业学报,2023,(02):489.[doi:doi:10.3969/j.issn.1000-4440.2023.02.022]
　GUO Feng,SUN Ying,AN Rong-hui,et al.Effects of green light-emitting diode light treatment on the quality and antioxidant capacity of postharvest green cabbage (Brassica oleracea var. capitata L.)[J].,2023,(07):489.[doi:doi:10.3969/j.issn.1000-4440.2023.02.022]
[3]韩冰,高文瑞,孙艳军,等.不同LED光照对番茄幼苗生理特性及定植后开花结果的影响[J].江苏农业学报,2024,(12):2219.[doi:doi:10.3969/j.issn.1000-4440.2024.12.004]
　HAN Bing,GAO Wenrui,SUN Yanjun,et al.Effects of different LED illumination on physiological characteristics of tomato seedlings and flowering and fruiting after planting[J].,2024,(07):2219.[doi:doi:10.3969/j.issn.1000-4440.2024.12.004]

备注/Memo

备注/Memo:
收稿日期：2024-08-08基金项目：福建省科技计划项目(2023S0026)；福建省省级财政花卉产业发展项目(优良品种创新激励)[闽财资环指(2022)47号]作者简介：林辉锋(1984-),男,福建莆田人,硕士,副研究员,主要研究方向为花卉生物技术。(E-mail)289687622@qq.com通讯作者：周辉明,(E-mail)120914051@qq.com
更新日期/Last Update: 2025-08-19