[1]薛园园,李嘉欣,吴甜甜,等.纳米金对桔梗生长和叶绿素荧光特性的影响[J].江苏农业学报,2025,(06):1223-1232.[doi:doi:10.3969/j.issn.1000-4440.2025.06.019]
　XUE Yuanyuan,LI Jiaxin,WU Tiantian,et al.Effects of gold nanoparticles on the growth and chlorophyll fluorescence characteristics of Platycodon grandiflorus[J].,2025,(06):1223-1232.[doi:doi:10.3969/j.issn.1000-4440.2025.06.019]
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纳米金对桔梗生长和叶绿素荧光特性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1223-1232
栏目:
园艺
出版日期:
2025-06-30

文章信息/Info

Title:
Effects of gold nanoparticles on the growth and chlorophyll fluorescence characteristics of Platycodon grandiflorus
作者:
薛园园1; 3; 李嘉欣2; 吴甜甜3; 夏可儿3; 李双晴3; 程颖3; 陈延松3
(1.安徽大学生命科学学院,安徽合肥230601；2.安徽大学资源与环境工程学院,安徽合肥230601；3.合肥师范学院生物与食品工程学院,安徽合肥230061)
Author(s):
XUE Yuanyuan1; 3; LI Jiaxin2; WU Tiantian3; XIA Keer3; LI Shuangqing3; CHENG Ying3; CHEN Yansong3
(1.School of Life Sciences, Anhui University, Hefei 230601, China;2.School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China;3.School of Biology and Food Engineering, Hefei Normal University, Hefei 230061, China)
关键词:
桔梗; 纳米金; 叶绿素荧光参数; 快速光响应曲线
Keywords:
Platycodon grandiflorus; gold nanoparticles; chlorophyll fluorescence parameters; rapid light response curve
分类号:
Q949.783.2
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.019
文献标志码:
A
摘要:
为探究纳米金对桔梗(Platycodon grandiflorus)生长、叶绿素荧光特性的影响,本研究以二年生桔梗为试验材料,对桔梗叶片喷施质量浓度为8 mg/L、16 mg/L、32 mg/L的纳米金(粒径约35 nm)溶液,测量桔梗生长指标、叶绿素荧光参数。结果表明,喷施纳米金3次时,8 mg/L纳米金处理桔梗光化学猝灭系数(qL)显著高于对照(P＜0.05),8 mg/L和16 mg/L纳米金处理桔梗最大电子传递速率(ETRmax)显著高于对照(P＜0.05)。喷施纳米金4次时,32 mg/L纳米金处理桔梗光系统Ⅱ实际量子产量[Y(Ⅱ)]、电子传递速率(ETR)、光化学猝灭系数(qL)显著低于对照(P＜0.05),调节性能量耗散的量子产量[Y(NPQ)]、叶绿素相对含量(SPAD)、光响应曲线的初始斜率显著高于对照(P＜0.05)。喷施纳米金4次,与对照相比,8 mg/L纳米金处理桔梗单根干重和侧根数分别显著提高了114.29%、115.47%(P＜0.05)；16 mg/L纳米金处理桔梗单根干重和主根长分别显著提高了68.57%、28.30%(P＜0.05)。纳米金喷施次数对桔梗光系统Ⅱ实际量子产量[Y(Ⅱ)]、电子传递速率(ETR)、光化学猝灭系数(qL)、调节性能量耗散的量子产量[Y(NPQ)]、光系统Ⅱ最大光化学量子产量[Fv/Fm]、最大电子传递速率(ETRmax)、SPAD值影响极显著(P＜0.01)。纳米金质量浓度对桔梗光化学猝灭系数(qL)、最大电子传递速率(ETRmax)影响极显著(P＜0.01),纳米金喷施次数和纳米金质量浓度的联合作用对桔梗光化学猝灭系数(qL)的影响极显著(P＜0.01)。研究结果表明适宜质量浓度的纳米金可提高桔梗叶片光合能力,促进植株生长以及侧根发生,显著提高桔梗根部生物量。回归分析结果表明,当纳米金质量浓度为12.98 mg/L时,桔梗根部生物量达到最大值,单根干重为0.65 g。本研究结果可为纳米金叶面肥的研发和应用提供理论支持。
Abstract:
To investigate the effects of gold nanoparticles (AuNPs) on the growth and chlorophyll fluorescence characteristics of Platycodon grandiflorus, this study used two-year-old Platycodon grandiflorus as the experimental material. The leaves of Platycodon grandiflorus were sprayed with AuNPs (with a particle size of 35 nm) solutions at mass concentrations of 8 mg/L, 16 mg/L, and 32 mg/L. The growth indices and chlorophyll fluorescence parameters of Platycodon grandiflorus were measured. The results showed that after three applications of AuNPs, the photochemical quenching coefficient (qL) of Platycodon grandiflorus treated with 8 mg/L AuNPs was significantly higher than that of the control (P＜0.05), and the maximum electron transport rates (ETRmax) of Platycodon grandiflorus treated with 8 mg/L and 16 mg/L AuNPs were significantly higher than that of the control (P＜0.05). After four applications of AuNPs, the actual quantum yield of photosystem Ⅱ [Y(Ⅱ)], electron transport rate (ETR) and photochemical quenching coefficient (qL) of Platycodon grandiflorus treated with 32 mg/L AuNPs were significantly lower than those of the control (P＜0.05), while the quantum yield of regulated energy dissipation [Y(NPQ)], the relative chlorophyll content (SPAD) and the initial slope of the light response curve (α) were significantly higher than those of the control (P＜0.05). After four applications of AuNPs, compared with the control, the single-root dry weight and lateral root number of Platycodon grandiflorus treated with 8 mg/L AuNPs increased significantly by 114.29% and 115.47%, respectively (P＜0.05); the single-root dry weight and main root length of Platycodon grandiflorus treated with 16 mg/L AuNPs increased significantly by 68.57% and 28.30%, respectively (P＜0.05). The number of AuNPs applications had an extremely significant effect on the the actual quantum yield of photosystem Ⅱ[Y(Ⅱ)], electron transport rate (ETR), photochemical quenching coefficient (qL), quantum yield of regulated energy dissipation [Y(NPQ)], the maximum photochemical quantum yield of photosystemⅡ(Fv/Fm)， the maximum electron transport rate (ETRmax) and SPAD of Platycodon grandiflorus (P＜0.01). The mass concentration of AuNPs had extremely significant effects on the photochemical quenching coefficient (qL) and the maximum electron transport rate (ETRmax) of Platycodon grandiflorus (P＜0.01). The combined effect of the number of AuNPs applications and the mass concentration of AuNPs on the photochemical quenching coefficient (qL) of Platycodon grandiflorus was extremely significant (P＜0.01). These results indicate that an appropriate mass concentration of AuNPs can enhance the photosynthetic capacity of Platycodon grandiflorus leaves, promote plant growth and lateral root development, and significantly increase the root biomass of Platycodon grandiflorus. Regression analysis showed that the root biomass of Platycodon grandiflorus reached its maximum value when the mass concentration of AuNPs was 12.98 mg/L, with a single-root dry weight of 0.65 g. This study provides theoretical support for the development and application of gold nanoparticle foliar fertilizers.

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备注/Memo

备注/Memo:
收稿日期：2024-09-04基金项目：2021年安徽省高校优秀拔尖人才培育项目(gxbjZD2021073)作者简介：薛园园(2001-),女,安徽亳州人,硕士研究生,主要从事植物学研究。(E-mail)3237131080@qq.com通讯作者：陈延松,(E-mail)ottffss7531_cn@126.com
更新日期/Last Update: 2025-07-16