[1]方欣妍,何秀琼,黄丹,等.薄壳山核桃MADS基因家族的全基因组鉴定及生物信息学分析[J].江苏农业学报,2025,(06):1080-1094.[doi:doi:10.3969/j.issn.1000-4440.2025.06.005]
　FANG Xinyan,HE Xiuqiong,HUANG Dan,et al.Genome-wide identification and bioinformatics analysis of the MADS gene family in Carya illinoinensis (Wangenh.) K. Koch[J].,2025,(06):1080-1094.[doi:doi:10.3969/j.issn.1000-4440.2025.06.005]
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薄壳山核桃MADS基因家族的全基因组鉴定及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1080-1094
栏目:
遗传育种·生理生化
出版日期:
2025-06-30

文章信息/Info

Title:
Genome-wide identification and bioinformatics analysis of the MADS gene family in Carya illinoinensis (Wangenh.) K. Koch
作者:
方欣妍1; 2; 何秀琼1; 2; 黄丹1; 2; 彭兵阳1; 2; 孙佳雯1; 2; 吕佳斌1; 2
(1.安徽农业大学林学与园林学院,安徽合肥230036；2.林木资源培育安徽省重点实验室,安徽合肥230036)
Author(s):
FANG Xinyan1; 2; HE Xiuqiong1; 2; HUANG Dan1; 2; PENG Bingyang1; 2; SUN Jiawen1; 2; LYU Jiabin1; 2
(1.School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China;2.Anhui Provincial Key Laboratory of Forest Resources and Silviculture, Hefei 230036, China)
关键词:
薄壳山核桃; MADS基因家族; 生物信息学; 表达分析
Keywords:
Carya illinoinensis (Wangenh.) K. Koch; MADS gene family; bioinformatics; expression analysis
分类号:
S664.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.005
文献标志码:
A
摘要:
MADS基因是一类重要的转录调控因子,具有广泛的生物学功能,参与多个物种的生长发育和信号传导,在多个物种中的生物学特性已被鉴定。本研究通过生物信息学方法对薄壳山核桃的MADS基因家族成员进行鉴定分析,最终鉴定出81个MADS基因家族成员,不均匀分布在16条染色体上,根据系统进化树分析结果MADS蛋白分为type-Ⅰ型(20个)和type-Ⅱ型(61个)。薄壳山核桃该家族基因具有较多顺式作用元件,植物激素响应元件、逆境胁迫响应元件和光响应元件等,研究发现这些作用元件广泛存在于MADS基因的启动子区域,表明MADS基因在薄壳山核桃适应环境变化及生长发育过程中充当重要角色。不同薄壳山核桃MADS家族基因在不同组织中和不同时期的表达量差异较大,如CiMADS46在茎、CiMADS7在果实具有较高表达量,CiMADS11的表达量随着时间推移递减。MADS基因具有不同的组织特异性表达模式,推测MADS基因家族可能在成花及果实发育过程中发挥重要作用,在不同发育阶段具有一定程度的功能多样性。本研究结果为进一步研究CiMADS基因功能与特性奠定了基础,并为其他物种的MADS基因家族成员的鉴定和表征提供参考。
Abstract:
The MADS genes are a class of important transcriptional regulatory factors, with extensive biological functions. They are involved in the growth, development, and signal transduction of multiple species, and their biological characteristics have been identified in various organisms. In this study, bioinformatics methods were employed to identify and analyze the MADS gene family members in Carya illinoinensis (Wangenh.) K. Koch. A total of 81 MADS gene family members were identified, which were unevenly distributed across 16 chromosomes. Based on the results of phylogenetic tree analysis, the MADS proteins were divided into type-Ⅰ(20) and type-Ⅱ(61). The genes of this family in Carya illinoinensis were found to contain a large number of cis-acting elements, including plant hormone response elements, stress response elements, and light response elements. The study revealed that these cis-acting elements were widely distributed in the promoter regions of the MADS genes, indicating that the MADS genes had played an important role in the adaptation to environmental changes and the growth and development of Carya illinoinensis. The expression levels of different MADS family genes in Carya illinoinensis varied greatly in different tissues and at different times. For example, CiMADS46 had a high expression level in the stem, CiMADS7 had a high expression level in the fruit, and the expression level of CiMADS11 decreased over time. The MADS genes were found to exhibit distinct tissue-specific expression patterns. It was speculated that the MADS gene family might play important roles in the processes of flower formation and fruit development, and they might have a certain degree of functional diversity at different developmental stages. The results of this study lay the foundation for further research on the functions and characteristics of CiMADS genes and provide a reference for the identification and characterization of MADS gene family members in other species.

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备注/Memo

备注/Memo:
收稿日期：2024-08-26基金项目：安徽省自然科学基金青年项目(2208085QC92)；安徽省高等学校科学研究项目(2024AH050484)；省级大学生创新创业训练计划项目(S202310364160)作者简介：方欣妍(2003-),女,江苏苏州人,本科,主要从事林木遗传育种与分子生物学研究。(E-mail)fxyyun@163.com通讯作者：吕佳斌,(E-mail)lvjiabin@ahau.edu.cn
更新日期/Last Update: 2025-07-16