[1]王一峰,胡文斌,陈耀年,等.不同干燥方法对核桃贮藏过程中内种皮褐变的影响[J].江苏农业学报,2025,(04):764-775.[doi:doi:10.3969/j.issn.1000-4440.2025.04.015]
 WANG Yifeng,HU Wenbin,CHEN Yaonian,et al.Effects of different drying methods on browning of walnut endotesta during storage[J].,2025,(04):764-775.[doi:doi:10.3969/j.issn.1000-4440.2025.04.015]
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不同干燥方法对核桃贮藏过程中内种皮褐变的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年04期
页码:
764-775
栏目:
园艺
出版日期:
2025-04-30

文章信息/Info

Title:
Effects of different drying methods on browning of walnut endotesta during storage
作者:
王一峰1胡文斌1陈耀年1赵淑玲1王明霞2
(1.陇南师范学院农林技术学院,甘肃成县742500;2.成县核桃科技服务中心,甘肃成县742500)
Author(s):
WANG Yifeng1HU Wenbin1CHEN Yaonian1ZHAO Shuling1WANG Mingxia2
(1.Technique College of Agriculture and Forestry, Longnan Normal University, Chengxian 742500, China;2.Chengxian Walnut Science and Technology Service Center, Chengxian 742500, China)
关键词:
核桃内种皮干燥方法贮藏褐变
Keywords:
walnutendotestadrying methodsstoragebrowning
分类号:
S664.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.04.015
文献标志码:
A
摘要:
为探讨干燥方法对核桃贮藏过程中内种皮褐变的影响,为核桃贮藏过程中内种皮褐变调控提供理论依据,本研究以核桃品种清香为试验材料,采用自然晒干(CK)、热风干燥(T1)、真空干燥(T2)及真空变温干燥(T3)4种干燥方法处理,测定贮藏过程中核仁外观及内种皮褐变指标变化,并采用通径分析法分析影响核桃内种皮褐变的主要因素。结果表明:随着贮藏时间的延长,各处理核仁颜色逐渐加深,褐变度增加,与CK相比,T2、T3处理核仁颜色较浅、褐变度较小;相对电导率和丙二醛(MDA)含量随贮藏时间延长逐渐增加,CK增幅最大,T2、T3处理增幅较小;核桃内种皮总酚含量在贮藏30 d时均有所下降,贮藏60 d时有所升高,贮藏90 d时又开始下降,但上升和下降的幅度都不大,整个贮藏期间核桃内种皮总酚含量维持较高水平,与CK相比,T2、T3处理核桃内种皮总酚含量总体较高;核桃内种皮多酚氧化酶(PPO)、超氧化物歧化酶(SOD)、Na+K+-ATP酶活性均呈先升后降的趋势,与CK相比,T2、T3处理核桃内种皮SOD、Na+K+-ATP酶活性总体较高,PPO活性总体较低;核桃内种皮过氧化物酶(POD)活性整体呈下降趋势,与CK相比,T2、T3处理核桃内种皮POD活性总体较高;过氧化氢酶(CAT)、脂氧合酶(LOX)活性均呈上升趋势,与CK相比,T2、T3处理CAT活性较高,LOX活性较低。通径分析结果表明:不同干燥方法处理的核桃在贮藏过程中影响核桃褐变的主要因素不同,PPO、POD及Na+K+-ATP酶活性是影响CK核桃褐变的主要因素;总酚含量、CAT活性、Na+K+-ATP酶活性是影响T1处理核桃褐变的主要因素;SOD、POD、Na+K+-ATP酶活性是影响T2处理核桃褐变的主要因素;总酚含量、POD活性、Na+K+-ATP酶活性是影响T3处理核桃褐变的主要因素。
Abstract:
To investigate the impact of drying methods on the browning of the endotesta during walnut storage and to provide a theoretical basis for the control of endotesta browning during walnut storage, this study used the walnut variety “Qingxiang” as the experimental material. Four drying methods were employed: natural sun-drying (CK), hot-air drying (T1), vacuum drying (T2), and variable-temperature vacuum drying (T3). During storage, changes in the appearance of the kernel and browning indices of the endotesta were measured. Path analysis was used to identify the main factors affecting endotesta browning in walnuts. The results indicated that with the extension of storage time, the color of the kernels in all treatments gradually darkened and the degree of browning increased. Compared with CK, the kernels in T2 and T3 treatments had lighter color and lower degree of browning. The relative electrical conductivity and the content of malondialdehyde (MDA) gradually increased with the extension of storage time. The increase was the highest in CK, while the increments in T2 and T3 treatments were relatively smaller. The total phenolic content in the walnut endotesta decreased at 30 days of storage, increased at 60 days, and then decreased again at 90 days. However, the magnitude of these increases and decreases was not significant. Throughout the storage period, the total phenolic content in the walnut endotesta remained at a relatively high level. Compared with CK, the total phenolic content in the endotesta of walnuts in T2 and T3 was generally higher. The activities of polyphenol oxidase (PPO), superoxide dismutase (SOD) and Na+/K+-ATPase in walnut endotesta increased first and then decreased. Compared with CK, the activities of SOD and Na+/K+-ATPase in walnut endotesta under T2 and T3 treatments were generally higher, and the activity of PPO was generally lower. The peroxidase (POD) activity of walnut endotesta showed a downward trend as a whole. Compared with CK, the POD activity of walnut endotesta in T2 and T3 treatments was generally higher. The activities of catalase (CAT) and lipoxygenase (LOX) showed an upward trend. Compared with CK, the activity of CAT was higher and the activity of LOX was lower in T2 and T3 treatments. The path analysis results indicated that different drying methods had different effects on walnut browning during storage. For walnuts in the CK, the main factors influencing browning were the activities of PPO, POD, and Na+/K+-ATPase. For walnuts in T1 treatment, the main factors influencing browning were the total phenolic content, CAT activity, and Na+/K+-ATPase activity. For walnuts in T2 treatment, the main factors influencing browning were the activities of SOD, POD, and Na+/K+-ATPase. For walnuts in T3 treatment, the main factors influencing browning were the total phenolic content, POD activity, and Na+/K+-ATPase activity.

参考文献/References:

[1]彭宇航. 核桃果实冷藏期抗氧化代谢与褐变关系的研究[D]. 杨凌:西北农林科技大学,2022.
[2]王一峰,赵淑玲,王瀚,等. 不同核桃种质展叶期抗寒性的综合评价[J]. 经济林研究,2019,37(1):50-60.
[3]白鸽,王甄妮,朱丹实,等. 采后果实的果皮褐变机理及防褐变研究进展[J]. 包装工程,2021,42(5):80-87.
[4]叶妞. 气体协同控制青皮核桃果实褐变的效应及生理机制研究[D]. 杨凌:西北农林科技大学,2019.
[5]王艳颖,胡文忠,庞坤,等. 机械伤害引起果蔬褐变机理的研究进展[J]. 食品工业科技,2007,28(11):230-233.
[6]曾镇. 不同干制方式下竹荪营养品质的对比及褐变行为研究[D]. 成都:成都大学,2023.
[7]BARBOSA-CNOVAS G V, VEGA-MERCADO H. Dehydration of Foods[M]. London:Chapman & Hal,1996:114-117.
[8]王文倩,王晗琦,陈文,等. 不同干燥方法对核桃品质及不饱和脂肪酸稳定性的影响[J]. 食品科学技术学报,2015,33(1):59-64.
[9]PRAVEEN K D, UMESH H H, S UKUMAR D, et al. Infrared and hot-air drying of onions[J]. Journal of Food Processing and Preservation,2005,29(2):132-150.
[10]陈佳妮,罗耀华,孔慧,等. 热激处理对鲜切百合鳞茎片贮藏品质的影响[J]. 食品科学,2024,45(9):163-172.
[11]GAO H, CHAI H, CHENG N, et al. Effects of 24-epibrassinolide on enzymatic browning and antioxidant activity of fresh-cut lotus root slices[J]. Food Chemistry, 2017,217:45-51.
[12]KUMAR D, LADANIYA M S, GURJAR M, et al. Impact of drying methods on natural antioxidants,phenols and flavanones of immature dropped Citrus sinensis L.Osbeck fruis[J]. Scientific Reports,2022,12:6684.
[13]MA Y P, WANG C Y, LIU C B, et al. Physiochemical responses of the kernel quality, total phenols and antioxidant enzymes of walnut in different forms to the low-temperature storage[J]. Foods,2021,10(9):2027.
[14]郑龙. 板栗品种褐变度差异性及其与多酚氧化酶活性的相关性研究[D]. 合肥:安徽农业大学,2015.
[15]徐涓,张雯雯,李凯,等. 高温蒸汽处理对余甘子果汁贮藏期间的品质影响及褐变行为解析[J]. 食品科学,2019,40(23):246-252.
[16]IGHODARO O M, AKINLOYE O A. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase(GPX):their fundamental role in the entire antioxidant defence grid[J]. Alexandria Journal of Medicine,2018,54(4):287-293.
[17]MESBAH B, FARHAD P, MOHAMMAD A S, et al. Arginine treatment attenuates chilling injury of pomegranate fruit during cold storage by enhancing antioxidant system activity[J]. Postharvest Biology and Technology,2018,137:31-37.
[18]王一峰,宫峥嵘,胡文斌,等. 采收期对核桃贮藏期间内种皮褐变的影响[J]. 经济林研究,2024,42(3):255-263.
[19]FAN J, DU W, CHENG Q L, et al. Comparative transcriptomic analyses provide insights into the enzymatic browning mechanism of fresh-cut sand pear fruit[J]. Horticulturae,2021,7(11):502.
[20]BURDON J, LALLU N, FRANCIS K, et al. The susceptibility of kiwifruit to low temperature breakdown is associated with pre-harvest temperatures and at-harvest soluble solids content[J]. Postharvest Biology and Technology,2007,43(3):283-290.
[21]HO Q T, VERBOVEN P, VERLINDEN B E, et al. Controlled atmosphere storage may lead to local ATP deficiency in apple[J]. Postharvest Biology and Technology,2013,78:103-112.
[22]ZHENG X L, TIAN S P, GIDLEY M J, et al. Slowing the deterioration of mango fruit during cold storage by pre-storage application of oxalic acid[J]. Journal of Horticultural Science & Biotechnology,2007,82(5):707-714.
[23]胡康棣,彭湘君,姚改芳,等. 不同耐贮性甘薯的抗氧化特性差异[J]. 江苏农业科学,2021,49(16):162-168.
[24]林之林,郝田,于景金,等. 盐胁迫下3种外源物对高羊茅生理指标的影响[J]. 草业科学,2022,39(4):720-730.
[25]杨旭风,贾晓东,许梦洋,等. 薄壳山核桃采后种皮褐变生理机制的研究[J]. 果树学报,2022,39(9):1701-1709.
[26]万冰. 微加工百合鳞茎褐变机制及其调控研究[D]. 扬州:扬州大学,2018.
[27]李政红. 梨果褐变机理研究[D]. 保定:河北农业大学,2016.
[28]ZHANG C, JIN Y Z, LIU J Y, et al. The phylogeny and expression profiles of the lipoxygenase (LOX) family genes in the melon (Cucumis melo L.) genome[J]. Scientia Horticulturae,2014,170: 94-102.
[29]WANG M, SUM J, FENG W H, et al. Identification of ripening-related lipoxygenase in tomato fruit as blanching indicator enzyme[J]. Process Biochemistry,2008,43(9):932-936.
[30]BARGMANN B O, MUNNIK T. The role of phospholipase D in plant stress responses[J]. Current Opinion in Plant Biology,2006,9(5):515-522.
[31]YIFEN L, HETONG L, SHEN Z, et al. The role of active oxygen metabolism in hydrogen peroxide-induced pericarp browning of harvested longan fruit[J]. Postharvest Biology and Technology,2014,96:42-48.
[32]LIU H, SONG L L, YOU Y L, et al. Cold storage duration affects litchi fruit quality, membrane permeability, enzyme activities and energy charge during shelf time at ambient temperature[J]. Postharvest Biology and Technology,2011,60(1):24-30.
[33]NUTTHACHAI P, YOSHIHIKO S, SUMIKO S, et al. A novel postharvest UV-C treatment to reduce chilling injury (membrane damage, browning and chlorophyll degradation) in banana peel[J]. Scientia Horticulturae,2011,130(1):73-77.
[34]LIU H, JIANG Y M, LUO Y B, et al. A simple and rapid determination of ATP, ADP and AMP concentrations in pericarp tissue of litchi fruit by high performance liquid chromatography[J]. Food Technology and Biotechnology,2006,4(44):531-534.
[35]邓华聪,邱鸿鑫,汪恕萍,等. 糖尿病红细胞膜Na+K+-ATP酶和Mg2+-ATP酶活性改变与脂质过氧化的关系[J]. 重庆医科大学学报,1996,21(2):111-114.
[36]刘梦竹,周宏胜,胡花丽,等. 采收期对黄金梨果心褐变和膜脂过氧化的影响[J]. 山西农业大学学报(自然科学版),2020,40(1):38-43.

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备注/Memo

备注/Memo:
收稿日期:2024-05-09基金项目:甘肃省自然科学基金项目(22JR5RK1049);甘肃省高校教师创新基金项目(2024B-333);甘肃省高等学校创新创业教育改革项目[甘教高函(2023)14号];甘肃省大学生就业创业能力提升工程项目[甘教学函(2023)29号]作者简介:王一峰(1986-),男,甘肃礼县人,硕士,副教授,研究方向为植物生理生态。(E-mail)wangyifeng0305@163.com
更新日期/Last Update: 2025-05-26