[1]韩凤,黄靖,章文伟,等.多花黄精白绢病病原菌鉴定及室内药剂筛选[J].江苏农业学报,2024,(12):2244-2253.[doi:doi:10.3969/j.issn.1000-4440.2024.12.007]
　HAN Feng,HUANG Jing,ZHANG Wenwei,et al.Pathogen identification and indoor fungicide screening of southern blight of Polygonatum cyrtonema[J].,2024,(12):2244-2253.[doi:doi:10.3969/j.issn.1000-4440.2024.12.007]
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多花黄精白绢病病原菌鉴定及室内药剂筛选()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
2244-2253
栏目:
植物保护
出版日期:
2024-12-30

文章信息/Info

Title:
Pathogen identification and indoor fungicide screening of southern blight of Polygonatum cyrtonema
作者:
韩凤1; 黄靖2; 章文伟1; 林茂祥1; 肖忠1; 宋旭红2
(1.重庆市药物种植研究所/重庆市道地药材规范化生产工程技术研究中心,重庆408435；2.重庆市中药研究院,重庆400065)
Author(s):
HAN Feng1; HUANG Jing2; ZHANG Wenwei1; LIN Maoxiang1; XIAO Zhong1; SONG Xuhong2
(1.Chongqing Institute of Medical Plant Cultivation/Chongqing Engineering Research Center for Standardized Production of Genuine Medicinal Materials, Chongqing 408435, China;2.Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China)
关键词:
多花黄精; 白绢病; 病原菌; 罗耳阿太菌
Keywords:
Polygonatum cyrtonema; southern blight; pathogen; Athelia rolfsii
分类号:
S435.672
DOI:
doi:10.3969/j.issn.1000-4440.2024.12.007
文献标志码:
A
摘要:
为明确多花黄精白绢病病原菌的种类及生物学特性,筛选适宜的杀菌剂,本研究从重庆市南川区采集多花黄精白绢病病株,采用菌核分离法,分离纯化得到多花黄精白绢病病原菌菌株BJB,依据柯赫氏法则验证其致病性,通过形态学及分子生物学鉴定病原菌种类,通过生物学试验明确病原菌的适宜生长条件,并通过室内药效试验筛选适宜杀菌剂。结果表明,本研究分离纯化得到的白绢病病原菌菌株BJB为罗耳阿太菌（Athelia rolfsii）。多花黄精白绢病病原菌的适宜生长条件为马铃薯蔗糖琼脂培养基(PSA),温度25 ℃,pH值4,碳源蔗糖,氮源硝酸钠,暗培养。多花黄精白绢病病原菌菌丝和菌核的致死温度分别为45 ℃和55 ℃。杀菌剂戊唑醇对多花黄精白绢病病原菌的抑制效果最好,半抑制浓度(EC50)为0.420 μg/mL；咯菌腈、萎锈灵和嘧菌酯的抑制效果其次,EC50分别为1.750 μg/mL、1.991 μg/mL和2.940 μg/mL。本研究结果为多花黄精白绢病病害的诊断与防治提供依据。
Abstract:
In order to clarify the species and biological characteristics of the pathogen of southern blight of Polygonatum cyrtonema and screen suitable fungicides, the diseased plants of Polygonatum cyrtonema were collected from Nanchuan District of Chongqing City, and the pathogen strain BJB of Polygonatum cyrtonema was isolated and purified by sclerotia separation method. The pathogenicity was verified according to Koch’s rule. The species of pathogenic bacteria were identified by morphology and molecular biology. The suitable growth conditions of pathogenic bacteria were determined by biological test, and the suitable fungicides were screened by indoor efficacy test. The results showed that the strain BJB isolated and purified in this study was Athelia rolfsii. The suitable growth conditions of the pathogen were as follows:potato sucrose agar (PSA) medium, temperature 25 ℃, pH 4, sucrose as carbon source, sodium nitrate as nitrogen source, and dark culture. The lethal temperature of mycelium and sclerotium of the pathogen was 45 ℃ and 55 ℃, respectively. The fungicide tebuconazole showed the best inhibitory effect against the pathogen, and the median effective concentration (EC50) was 0.420 μg/mL. The EC50 values for fludioxonil, triadimefon and azoxystrobin were 1.750 μg/mL, 1.991 μg/mL and 2.940 μg/mL, respectively. The results of this study provide a basis for the diagnosis and prevention of southern blight disease of Polygonatum cyrtonema.

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备注/Memo

备注/Memo:
收稿日期：2024-03-16基金项目：重庆市基本科研业务专项(2023JK70-2512)；重庆市技术创新与应用发展专项重点项目（CSTB2024TIAD-KPX0049）；重庆市科卫联合中医药科研项目(2019zy023281)；重庆市卫生健康委员会中医药科技项目(zy201702147)作者简介：韩凤(1980-),女,重庆人,本科,正高级农艺师,主要从事药用植物栽培研究。(E-mail)hanfengasdf@126.com通讯作者：宋旭红,(E-mail)songxuhong@cqacmm.com
更新日期/Last Update: 2025-01-23