[1]李世金,林硕,马称心,等.生物质灰加生物菌剂对烟草根际土壤微生物群落的影响以及对青枯病的防治效果[J].江苏农业学报,2024,(12):2237-2243.[doi:doi:10.3969/j.issn.1000-4440.2024.12.006]
　LI Shijin,LIN Shuo,MA Chenxin,et al.Effects of combined application of biomass ash and bio-bacterial agents on the microbial community in tobacco rhizosphere soil and the control effect on bacterial wilt[J].,2024,(12):2237-2243.[doi:doi:10.3969/j.issn.1000-4440.2024.12.006]
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生物质灰加生物菌剂对烟草根际土壤微生物群落的影响以及对青枯病的防治效果()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
2237-2243
栏目:
植物保护
出版日期:
2024-12-30

文章信息/Info

Title:
Effects of combined application of biomass ash and bio-bacterial agents on the microbial community in tobacco rhizosphere soil and the control effect on bacterial wilt
作者:
李世金; 林硕; 马称心; 程廷明; 朱启法
(安徽皖南烟叶有限责任公司,安徽宣城242000)
Author(s):
LI Shijin; LIN Shuo; MA Chenxin; CHENG Tingming; ZHU Qifa
(Anhui Wannan Tobacco Co., Ltd., Xuancheng 242000, China)
关键词:
生物质灰; 生物菌剂; 烟草; 根际微生物; 烟草青枯病
Keywords:
biomass ash; bio-bacterial agents; tobacco; rhizosphere microorganism; tobacco bacterial wilt
分类号:
S435.72
DOI:
doi:10.3969/j.issn.1000-4440.2024.12.006
文献标志码:
A
摘要:
为了探究生物质灰和不同生物菌剂配施对烟草根际土壤微生物群落的影响,以及对烟草青枯病的防控作用,本研究先采用高通量测序方法研究了生物质灰配施生物菌剂对土壤微生物群落多样性和微生物群落结构组成的影响。α多样性指数分析结果发现,生物质灰配施生物菌剂有利于提高烟草根际土壤微生物菌群丰度和菌群变化程度,生物质灰配施荧光假单胞菌菌剂的土壤Shannon指数和Simpson指数最高,配施光合细菌菌剂的烟草根际土壤Ace和Chao1值最高。通过主成分分析发现,生物质灰配施不同生物菌剂对根际土壤微生物的影响是类似的。另外,生物质灰配施生物菌剂可明显提高土壤中酸杆菌或放线菌相对丰度,其中生物质灰配施荧光假单胞菌菌剂和光合细菌菌剂土壤放线菌相对丰度更高。利用土壤微生物分离法研究生物质灰配施生物菌剂对微生物群落数量的影响,结果发现与CK相比生物质灰配施生物菌剂可以促进土壤微生物的生长繁殖,显著提高微生物群落数量,生物质灰配施光合细菌菌剂对微生物的生长繁殖促进作用最为显著。生物质灰配施生物菌剂后烟草青枯病病情指数明显低于CK,特别是生物质灰配施光合细菌菌剂的病情指数最低,防治效果最好。
Abstract:
In order to explore the effects of biomass ash and different bio-bacterial agents on the microbial community in tobacco rhizosphere soil and the prevention and control of tobacco bacterial wilt, this study first used high-throughput sequencing methods to explore the effects of biomass ash combined with bio-bacterial agents on soil microbial community diversity and microbial community structure. The results of α diversity index analysis showed that the application of biomass ash combined with bio-bacterial agents was beneficial to improve the abundance and change degree of microbial flora in tobacco rhizosphere soil. The Shannon index and Simpson index of soil treated with biomass ash combined with Pseudomonas fluorescens agent were the highest, and the Ace and Chao1 values of tobacco rhizosphere soil treated with biomass ash combined with the photosynthetic bacteria agent were the highest. Through principal component analysis, it was found that the effects of biomass ash combined with different bio-bacterial agents on rhizosphere soil microorganisms were similar. In addition, the relative abundance of Acidobacteria or Actinomycetes in soil could be significantly increased by the application of biomass ash combined with bio-bacterial agents. The relative abundance of soil actinomycetes was higher under the treatments of biomass ash combined with Pseudomonas fluorescens agent and photosynthetic bacteria agent. The effect of biomass ash combined with bio-bacterial agents on the number of microbial communities was studied by soil microbial separation method. The results revealed that compared with CK, biomass ash combined with bio-bacterial agents could promote the growth and reproduction of soil microorganisms and significantly increase the number of microbial communities. The biomass ash combined with the photosynthetic bacteria agent had the most significant effect on the growth and reproduction of microorganisms. The disease index of tobacco bacterial wilt after biomass ash combined with bio-bacterial agents treatment was significantly lower than that of CK. In particular, the disease index of tobacco bacterial wilt treated with biomass ash combined with the photosynthetic bacteria agent was the lowest and the control effect was the best.

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备注/Memo

备注/Memo:
收稿日期：2024-03-25基金项目：安徽皖南烟叶有限责任公司重点科技项目（20210563002）作者简介：李世金(1985-),男,安徽宣城人,本科,农艺师,主要从事烟叶生产研究。(E-mail)969156275@qq.com通讯作者：朱启法, (E-mail)zhuqifa2023@163.com
更新日期/Last Update: 2025-01-23