[1]曾建红,郑玉才,李丛艳,等.蜀兴1号肉兔与伊拉兔肉质差异的分子机制[J].江苏农业学报,2024,(09):1689-1700.[doi:doi:10.3969/j.issn.1000-4440.2024.09.013]
　ZENG Jianhong,ZHENG Yucai,LI Congyan,et al.Molecular mechanism of transcriptomic and metabolomic differences between the longissimus dorsi muscle of Shuxing No.1 rabbit and Ira rabbit[J].,2024,(09):1689-1700.[doi:doi:10.3969/j.issn.1000-4440.2024.09.013]
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蜀兴1号肉兔与伊拉兔肉质差异的分子机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年09期
页码:
1689-1700
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2024-09-30

文章信息/Info

Title:
Molecular mechanism of transcriptomic and metabolomic differences between the longissimus dorsi muscle of Shuxing No.1 rabbit and Ira rabbit
作者:
曾建红1; 2; 郑玉才2; 李丛艳1; 3; 郭志强1; 3; 杨锐1; 3; 郑洁1; 3; 李钰莹1; 3; 任永军1; 3; 雷岷1; 3; 谢晓红1; 3; 邝良德1; 3
(1.四川省畜牧科学研究院,四川成都610066；2.西南民族大学畜牧兽医学院,四川成都610041；3.动物遗传育种四川省重点实验室,四川成都610066)
Author(s):
ZENG Jianhong1; 2; ZHENG Yucai2; LI Congyan1; 3; GUO Zhiqiang1; 3; YANG Rui1; 3; ZHENG Jie1; 3; LI Yuying1; 3; REN Yongjun1; 3; LEI Min1; 3; XIE Xiaohong1; 3; KUANG Liangde1; 3
(1.Sichuan Animal Science Academy, Chengdu 610066, China;2.College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;3.Animal Genetics and Breeding Key Laboratory of Sichuan Province, Chengdu 610066, China)
关键词:
转录组学; 代谢组学; 蜀兴1号肉兔; 伊拉兔; 肉质
Keywords:
transcriptomics; metabolomics; Shuxing No.1 rabbit; Ira rabbit; meat quality
分类号:
S829.1
DOI:
doi:10.3969/j.issn.1000-4440.2024.09.013
文献标志码:
A
摘要:
为明确蜀兴1号肉兔(SX)与伊拉兔(IRA)肉质差异及差异形成分子机制,本研究通过采集2个品种兔背最长肌样品,进行肉质性状测定及转录组测序和代谢组分析,筛选差异表达基因和差异代谢物并进行功能富集分析。结果表明,蜀兴1号肉兔的肌纤维直径和滴水损失率显著低于伊拉兔,而肌纤维密度、熟肉率和肌内脂肪含量显著高于伊拉兔。蜀兴1号肉兔与伊拉兔转录组中共筛选出81个差异表达基因,其中蜀兴1号肉兔中51个基因上调,30个基因下调。SMTNL1、PM20D2和EDN1等可能是导致两种兔肉品质差异的基因。差异表达基因显著富集于cAMP信号传导途径。挥发性代谢组学比较共得到12种差异显著代谢物,均在蜀兴1号肉兔中上调,其中,2-十一烯醛、4-乙基辛酸、(E)-2-壬烯醛、鸟氨酸和十一醛等代谢物与肉质风味正相关,挥发性代谢物显著富集于2-氧代羧酸代谢、ABC转运蛋白和精氨酸生物合成等KEGG代谢通路。广泛靶向代谢组学比较分析共得到15种差异显著代谢物,其中蜀兴1号肉兔中7种代谢物上调,8种下调,2个品种兔肉中γ-L-谷氨酸-L-谷氨酰胺、L-谷氨酸-L-谷氨酰胺和溶血磷脂酰胆碱(LPC)等肉质风味相关物质的含量差异较大,广泛靶向代谢物显著富集于咖啡因代谢和昼夜节律夹带等KEGG代谢通路。本研究得到的差异表达基因和差异代谢物可为进一步的优质兔养殖和优质兔选育提供参考依据。
Abstract:
In order to clarify the difference of meat quality between Shuxing No.1 rabbit (SX) and Ira rabbit (IRA) and the molecular mechanism of the difference formation, the longissimus dorsi muscle samples of two varieties of rabbits were collected for meat quality trait determination, transcriptome sequencing and metabolomics analysis, and differentially expressed genes and differentially expressed metabolites were screened and their functional enrichment analysis was performed. The results showed that the muscle fiber diameter and drip loss rate of SX were significantly lower than those of IRA, while the muscle fiber density, cooked meat rate and intramuscular fat content were significantly higher than those of IRA. A total of 81 differentially expressed genes were screened in the transcriptome of SX and IRA. Among them, 51 genes were up-regulated and 30 genes were down-regulated in SX. SMTNL1, PM20D2 and EDN1 might be the genes that lead to the difference in meat quality between SX and IRA. The differentially expressed genes were significantly enriched in the cAMP signaling pathway. A total of 12 significantly different metabolites were obtained by volatile metabolomics comparison, all of which were up-regulated in SX. Among them, metabolites such as 2-undecenal, 4-ethyloctanoic acid, (E)-2-nonenal, ornithine and undecanal were positively correlated with meat flavor. The volatile metabolites were significantly enriched in KEGG metabolic pathways such as 2-oxocarboxylic acid metabolism, ABC transporter and arginine biosynthesis. A total of 15 significantly different metabolites were obtained by extensive targeted metabolomics comparative analysis. Among them, seven metabolites were up-regulated and eight metabolites were down-regulated in SX. The contents of meat flavor-related substances such as γ-L-glutamic acid-L-glutamine, L-glutamic acid-L-glutamine and lysophosphatidylcholine (LPC) in SX and IRA were significantly different. Widely targeted metabolites were significantly enriched in KEGG metabolic pathways such as caffeine metabolism and circadian rhythm entrainment. The differentially expressed genes and differential metabolites obtained in this study can provide a reference for further high-quality rabbit breeding.

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备注/Memo

备注/Memo:
收稿日期：2023-09-18基金项目：国家兔产业技术体系项目(CARS-43-D-1)；四川省财政运行专项(SASA2024CZYX004)；四川省“十四五”育种攻关项目(2021YFYZ0033)；四川省科研院所科技成果转化项目(2023N22J0001)作者简介：曾建红(1998-),女,四川绵阳人,硕士,主要从事家兔繁殖与饲养管理。(E-mail)1762725547@qq.com通讯作者：邝良德,(E-mail)215640832@qq.com
更新日期/Last Update: 2024-11-17