[1]高子文,贾智彤,王利晖,等.低共熔溶剂提取绿茶中茶多酚工艺优化及茶多酚提取液的抗氧化活性分析[J].江苏农业学报,2024,(07):1330-1342.[doi:doi:10.3969/j.issn.1000-4440.2024.07.020]
　GAO Ziwen,JIA Zhitong,WANG Lihui,et al.Process optimization of tea polyphenols extraction from green tea by deep eutectic solvents and analysis of antioxidant activity[J].,2024,(07):1330-1342.[doi:doi:10.3969/j.issn.1000-4440.2024.07.020]
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低共熔溶剂提取绿茶中茶多酚工艺优化及茶多酚提取液的抗氧化活性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年07期
页码:
1330-1342
栏目:
加工贮藏·质量安全
出版日期:
2024-07-30

文章信息/Info

Title:
Process optimization of tea polyphenols extraction from green tea by deep eutectic solvents and analysis of antioxidant activity
作者:
高子文; 贾智彤; 王利晖; 刘羽妍; 谢子菁; 刘洋; 滕杰
(江西农业大学农学院,江西南昌330045)
Author(s):
GAO Ziwen; JIA Zhitong; WANG Lihui; LIU Yuyan; XIE Zijing; LIU Yang; TENG Jie
(School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang 330045, China)
关键词:
低共熔溶剂; 茶多酚; 提取工艺; 响应面法; 抗氧化活性
Keywords:
deep eutectic solvents (DES); tea polyphenols; extraction technology; response surface methodology; antioxidant activity
分类号:
S571.1
DOI:
doi:10.3969/j.issn.1000-4440.2024.07.020
文献标志码:
A
摘要:
低共熔溶剂(Deep eutectic solvents,DES)作为一种绿色环保、高效无毒溶剂而被广泛应用在生物提取和化学制备领域。本研究拟采用DES提取绿茶中多酚类物质，首先合成5种不同类型的DES,进行傅里叶变换红外光谱(FT-IR)与黏度值的物理表征,并与以传统水溶液、70%甲醇作为溶剂所得茶多酚提取率进行比较,获得最佳DES类型。然后,通过单因素试验、响应面Box-Behnken设计试验优化用DES提取绿茶中茶多酚的工艺,并对提取前后的绿茶粉进行结构分析。结果显示,在提取过程中,当氯化胆碱与乳酸（DES-2）的含水率为30%、提取温度为75 ℃、提取时间29 min、料液比为1 g∶35 mL时,茶多酚提取率达25.16%,与模型预测结果相符。扫描电子显微镜(SEM)和X射线衍射(XRD)结果显示,在提取过程中茶粉的晶体结构无明显变化,但DES使茶粉表面形貌发生改变,使褶皱面增多,从而使茶粉与溶剂有更多接触面积,有利于多酚类物质的溶出。此外,将DES提取法与常用的超声波乙醇提取法、微波水提取法、热回流水提取法的结果进行比较发现,DES提取法在茶多酚提取率、部分儿茶素组分提取率上均具有显著优势(P<0.05)。用不同提取方法所得茶多酚提取液进行体外还原能力、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除活性试验,发现不同方法所得茶多酚提取液均表现出抗氧化活性并且其抗氧化活性呈现剂量依赖关系,以DES法获得的茶多酚提取液的抗氧化性最强,研究结果可为茶多酚提取及开发利用提供理论依据。
Abstract:
Deep eutectic solvents (DES) are widely used in biological and chemical fields as green, environmentally friendly, non-toxic and highly efficient solvents. In this study, deep eutectic solvents were used to extract polyphenols from green tea. Firstly, five different types of natural deep eutectic solvents were synthesized, and physical properties such as Fourier transform infrared spectroscopy (FT-IR) and viscosity values were characterized. Compared with extraction rates of tea polyphenols obtaining by using traditional water solution and 70% methanol organic solvent, the best DES type was acquired. Then, the single factor test and response surface Box-Behnken design test were used to optimize the extraction of green tea polyphenols based on DES, and the structure of green tea powder before and after extraction was analyzed. The results showed that when the moisture content of choline chloride and lactic acid (DES-2) was 30%, extraction temperature was 75 ℃, extraction time was 29 min, and solid-liquid ratio was 1∶35 (g/mL), the extraction rate of tea polyphenols was 25.16%, which was consistent with the prediction of the model. The results of scanning electron microscopy (SEM) and X-ray diffraction (XRD) showed that the crystal structure of tea powder did not change significantly during the extraction process, but the surface morphology of tea powder was changed greatly by DES, and more folds were formed on the surface of the tea powder, so that the tea powder had a larger contact area with the solvent, which was conducive to the dissolution of polyphenols. In addition, the DES extraction method was compared with the commonly used ultrasonic ethanol extraction method, microwave water extraction method, and hot reflux water extraction method. It was found that the DES extraction method had significant advantages in the extraction rates of tea polyphenols and some catechin components (P<0.05). The tea polyphenol extracts obtained by different extraction methods were tested for in vitro reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. It was found that the tea polyphenol extracts obtained by different methods showed antioxidant activity and the antioxidant activity was dose-dependent. The tea polyphenol extract obtained by DES method had the strongest antioxidant activity. The results of this study can provide a theoretical basis for the extraction and development of tea polyphenols.

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备注/Memo

备注/Memo:
收稿日期：2023-08-31基金项目：国家级大学生创新创业项目（202310410001）；国家自然科学基金项目 (32260791)作者简介：高子文（2001-），女，江西新余人，硕士研究生，主要研究方向为茶叶深加工。（E-mail） ziwen_G423@163.com通讯作者：滕杰，（E-mail）tengjie@jxau.edu.cn
更新日期/Last Update: 2024-09-14