[1]万军斌,王旺田,孙万仓,等.甘蓝型油菜BnWSD家族基因鉴定及其在干旱胁迫下的表达特征[J].江苏农业学报,2024,(07):1170-1181.[doi:doi:10.3969/j.issn.1000-4440.2024.07.003]
　WAN Junbin,WANG Wangtian,SUN Wancang,et al.Identification of BnWSD family genes in Brassica napus L. and their expression characteristics under drought stress[J].,2024,(07):1170-1181.[doi:doi:10.3969/j.issn.1000-4440.2024.07.003]
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甘蓝型油菜BnWSD家族基因鉴定及其在干旱胁迫下的表达特征()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年07期
页码:
1170-1181
栏目:
遗传育种·生理生化
出版日期:
2024-07-30

文章信息/Info

Title:
Identification of BnWSD family genes in Brassica napus L. and their expression characteristics under drought stress
作者:
万军斌1; 2; 王旺田1; 2; 孙万仓1; 2; 马骊1; 2; 武军艳1; 2; 刘丽君1; 2; 蒲媛媛1; 2; 张岩1; 2; 李治忠1; 2; 周蓉1; 2
(1.甘肃农业大学生命科学技术学院/甘肃省干旱生境作物学重点实验室,甘肃兰州730070；2.甘肃省油菜工程技术研究中心,甘肃兰州730070)
Author(s):
WAN Junbin1; 2; WANG Wangtian1; 2; SUN Wancang1; 2; MA Li1; 2; WU Junyan1; 2; LIU Lijun1; 2; PU Yuanyuan1; 2; ZHANG Yan1; 2; LI Zhizhong1; 2; ZHOU Rong1; 2
(1.College of Life Science and Technology of Gansu Agricultural University/Key Laboratory of Arid Habitat Crop Science of Gansu Province, Lanzhou 730070, China;2.Gansu Rape Engineering Technology Research Center, Lanzhou 730070, China)
关键词:
甘蓝型油菜; WSD基因家族; 鉴定; 干旱胁迫
Keywords:
Brassica napus; WSD gene family; identification; drought stress
分类号:
S634.301
DOI:
doi:10.3969/j.issn.1000-4440.2024.07.003
文献标志码:
A
摘要:
干旱胁迫能诱导作物蜡酯合成酶/二酰甘油酰基转移酶基因WSD(wax synthase/diacylglycerol acyltransferase)的表达。为明确甘蓝型油菜BnWSD家族基因的理化性质及其对干旱胁迫的响应特征,本研究基于甘蓝型油菜转录组数据库,利用生物信息学方法从甘蓝型油菜基因组中筛选BnWSD家族基因并对其结构、染色体定位、系统进化树及其编码蛋白质的理化性质、结构、保守基序、顺式作用元件等进行分析,并以陇油10号为试验材料,通过干旱胁迫处理明确BnWSD家族基因成员的表达特性。结果表明,甘蓝型油菜基因组中共鉴定到36个BnWSD家族基因,可分为3个亚家族,其编码蛋白质的氨基酸数量为105～538个,等电点为6.32～9.63；36个BnWSD家族基因共有8个保守基序,每个基因含有2～8个保守基序,motif 2是该家族共有的功能基序,在进化上较为保守；甘蓝型油菜BnWSD基因启动子区域存在11个与光响应、激素应答、干旱胁迫应答等相关的顺式作用元件。甘蓝型油菜不同器官中BnWSD基因的相对表达量存在差异,叶和茎中相对表达量较高,而根和花中的相对表达量较低或不表达。随着干旱程度加深,甘蓝型油菜叶片中BnWSD1-1、BnWSD2、BnWSD8、BnWSD9、BnWSD12、BnWSD18-1、BnWSD20-1、BnWSD24-1等基因的相对表达量呈增加趋势。综上,BnWSD基因与甘蓝型油菜抗旱性能密切相关,本研究结果为进一步解析甘蓝型油菜抗旱机制、明确BnWSD基因家族在表皮蜡质累积中的作用及甘蓝型油菜抗旱性品种选育提供了依据。
Abstract:
Drought stress can induce the expression of crop wax synthase/diacylglycerol acyltransferase (WSD) gene. In order to clarify the physical and chemical properties of BnWSD family genes in Brassica napus and their response characteristics to drought stress, this study used bioinformatics methods to screen BnWSD family genes from the B. napus genome and analyzed their structure, chromosome location, phylogenetic tree and the physical and chemical properties, structure, conserved motifs and cis-acting elements of their encoding proteins, based on the B. napus transcriptome database. The expression characteristics of BnWSD family genes were determined by drought stress treatment with Longyou 10 as the experimental material. The results showed that a total of 36 BnWSD family genes were identified in the genome of B.napus, of which could be divided into three subfamilies. The number of amino acids of encoded proteins of 36 BnWSD family genes ranged from 105 to 538, and the isoelectric point ranged from 6.32 to 9.63. There were eight conserved motifs in 36 BnWSD family genes, and each gene contained two to eight conserved motifs, and motif 2 was a common functional motif in the BnWSD family genes, which was relatively conserved in evolution. There were 11 cis-acting elements related to light response, hormone response and drought stress response in the promoter region of BnWSD gene in B. napus. The relative expression levels of BnWSD gene in different organs of B.napus were different. The relative expression levels in leaves and stems were relatively higher, while the relative expression levels in roots and flowers were relatively lower or not expressed. With the deepening of drought, the relative expression levels of BnWSD1-1, BnWSD2, BnWSD8, BnWSD9, BnWSD12, BnWSD18-1, BnWSD20-1 and BnWSD24-1 in leaves of B.napus showed an increasing trend. In summary, the BnWSD gene was closely related to the drought resistance of B.napus. The results of this study provide a basis for further analysis of the drought resistance mechanism of B.napus and clarifying the role of the BnWSD gene family in epidermal wax accumulation and the breeding of drought-resistant B.napus varieties.

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备注/Memo

备注/Memo:
收稿日期：2023-07-31基金项目：甘肃省教育厅高等学校创新基金项目(2022A-054)；甘肃省科技重大专项(22ZD6NA009)；2023年甘肃省级现代寒旱农业科技支撑项目(KJZC-2023-12)；国家油菜产业技术体系项目(CARS-12)作者简介：万军斌(1997-),男,甘肃平凉人,硕士研究生,主要从事油菜抗旱生理的研究。(E-mail)2107106686@qq.com通讯作者：王旺田,(E-mail)wtwang@gsau.edu.cn；孙万仓,(E-mail)18293121851@163.com
更新日期/Last Update: 2024-09-14