[1]刘财国,吕水源,于文涛,等.北苑贡茶茶树种质遗传多样性及其与青心乌龙茶树的亲缘关系[J].江苏农业学报,2024,(05):935-943.[doi:doi:10.3969/j.issn.1000-4440.2024.05.018]
　LIU Caiguo,LYU Shuiyuan,YU Wentao,et al.Genetic diversity of Beiyuan tribute tea germplasms and their genetic relationship with Qingxinwulong[J].,2024,(05):935-943.[doi:doi:10.3969/j.issn.1000-4440.2024.05.018]
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北苑贡茶茶树种质遗传多样性及其与青心乌龙茶树的亲缘关系()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年05期
页码:
935-943
栏目:
园艺
出版日期:
2024-05-30

文章信息/Info

Title:
Genetic diversity of Beiyuan tribute tea germplasms and their genetic relationship with Qingxinwulong
作者:
刘财国1; 吕水源2; 于文涛2; 潘宏英3; 王攀1; 严林华3; 朱艳宇1; 金珊1; 叶乃兴1
(1.福建农林大学园艺学院,福建福州350002；2.福州海关技术中心,福建福州350001；3.建瓯市茶业发展中心,福建建瓯353100)
Author(s):
LIU Caiguo1; LYU Shuiyuan2; YU Wentao2; PAN Hongying3; WANG Pan1; YAN Linhua3; ZHU Yanyu1; JIN Shan1; YE Naixing1
(1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Technology Centre of Fuzhou Customs District, Fuzhou 350001, China;3.Jian’ou Tea Industry Development Center, Jian’ou 353100, China)
关键词:
北苑贡茶; 青心乌龙; 茶树; 种质资源; 遗传多样性
Keywords:
Beiyuan tribute tea; Qingxinwulong; Camellia sinensis; germplasm resources; genetic diversity
分类号:
S571.1
DOI:
doi:10.3969/j.issn.1000-4440.2024.05.018
摘要:
为更好地发掘、保护和利用北苑贡茶茶树种质资源,采用EST-SNP分子标记技术对北苑贡茶种质遗传多样性及其与青心乌龙的亲缘关系进行分析。筛选出49个多态性强的SNP位点,适用于鉴定北苑贡茶茶树种质基因分型以及分析其与青心乌龙的亲缘关系,并进行PCoA主成分分析、层级进化树构建和遗传距离分析。结果表明,北苑贡茶茶树种质具有丰富的遗传多样性,其6个茶树种质群体间的遗传距离为0.021～0.137；北苑贡茶核心产地福建省建瓯市东峰镇的百年乌龙茶园为有性茶树-无性茶树混合种植的古茶园；青心乌龙茶树(中国台湾)、矮脚乌龙茶树(武夷山)与北苑贡茶的百年乌龙茶树种质亲缘关系密切。根据亲缘关系分析推测,青心乌龙茶树(中国台湾)、软枝乌龙茶树(中国台湾)和矮脚乌龙茶树(武夷山)可能均源自建瓯北苑贡茶产区。
Abstract:
To enhance the excavation, protection and utilization of excellent Beiyuan tribute tea germplasm resources, the genetic diversity of Beiyuan tribute tea germplasms and their genetic relationship with Qingxinwulong were analyzed using the EST-SNP molecular marker technique. Forty-nine SNP loci with strong polymorphism were screened out, which were suitable for identifying the genotyping of Beiyuan tribute tea germplasm and analyzing its genetic relationship with Qingxinwulong, and the PCoA principal component analysis, hierarchical phylogenetic tree construction and genetic distance analysis were carried out. The results showed that the germplasm resources of Beiyuan tribute tea had abundant genetic diversity, and the genetic distance among the six tea groups ranged from 0.021 to 0.137. The century-old oolong tea garden in Dongfeng Town, the core producing area of Beiyuan tribute tea, was an ancient tea garden with mixed planting of sexual and asexual. Qingxinwulong (Taiwan) and Aijiaowulong (Wuyishan) were closely related to the centennial oolong germplasm of Beiyuan tribute tea. According to the analysis of genetic relationship, it was speculated that Qingxinwulong (Taiwan), Ruanzhiwulong (Taiwan) and Aijiaowulong (Wuyishan) might be derived from Jian’ou Beiyuan tribute tea producing area.

参考文献/References:

[1]阚能才. 茶树起源与川渝野生茶树分布研究[J]. 西南农业学报,2013,26(1):382-385.
[2]巩志,姚月明. 建茶史微[J]. 农业考古,1995(4):192-201.
[3]潘宏英. 建瓯市茶产业发展现状与思考[J]. 中国农技推广,2021,37(1):35-37.
[4]赖少波. 北苑御焙名冠天下——《建瓯茶志》总述选萃[J]. 中国茶叶,2017,39(12):37-39.
[5]刘祖陛. 宋代建茶文化初探[J]. 福建史志,2019(4):1-6.
[6]肖正广,申长锋. 试析宋子安《东溪试茶录》的现实意义[J]. 中国茶叶加工,2017(增刊2):61-66.
[7]周理飞. 建瓯茶区优异种质资源保护与利用的实践[J]. 福建茶叶,2011,33(1):26-28.
[8]赖少波. 建瓯茶志[M]. 福州:福建科学技术出版社,2017:34-55.
[9]巩志. 建茶的传播[J]. 福建茶叶,2003,(4):46.
[10]郭琳,郭雅玲,洪翠云. 浅谈闽台茶业的渊源与交流[J]. 茶叶科学技术,2008,(1):52-54.
[11]LIN Y, YU W T, ZHOU L, et al. Genetic diversity of oolong tea (Camellia sinensis) germplasms based on the nanofluidic array of single-nucleotide polymorphism (SNP) markers[J]. Tree Genetics & Genomes,2020,16(1):1-14.
[12]罗祥宗,胡云飞,吴淋慧,等. 茶树叶绿体基因组SNP分子标记的初步研究[J]. 茶叶科学,2022,42(6):768-778.
[13]LIN Y, YU W T, CAI C P, et al. Rapid varietal authentication of oolong tea products by microfluidic-based SNP genotyping[J]. Food research international,2022,162(A):111970.
[14]LIU C G, YU W T, CAI C P, et al. Genetic diversity of tea plant [Camellia sinensis (L.) kntze] germplasm resources in Wuyi Mountain of China based on single nucleotide polymorphism (SNP) markers[J]. Horticulturae,2022,8(10):932-944.
[15]樊晓静,于文涛,蔡春平,等. 利用SNP标记构建茶树品种资源分子身份证[J]. 中国农业科学,2021,54(8):1751-1772.
[16]李锦生. 浅谈建瓯北苑贡茶文化的挖掘与弘扬[J]. 中国茶叶,2020,42(4):67-70.
[17]郑安儒,陈光武,徐 凯,等. 建瓯地区13个不同茶树品种制成乌龙茶品质差异研究[J]. 茶叶学报,2022,63(2):73-81.
[18]裴朝鉴. 茶树炭疽病的发生原因及防治措施[J]. 福建农业科技,2012(2):34-35.
[19]FANG W P, MEINHARDT L W, TAN H W, et al. Varietal identification of tea (Camellia sinensis) using nanofluidic array of single nucleotide polymorphism (SNP) markers[J]. Horticulture Research,2014,1(1):1-8.
[20]陈林海,钟吉茂,何燕燕,等. 福安地方茶树种质亲缘关系与遗传多样性[J]. 茶叶通讯,2022,49(2):173-180.
[21]WEI K, WANG X C, HAO X Y, et al.Development of a genome-wide 200K SNP array and its application for high-density genetic mapping and origin analysis of Camellia sinensis[J]. Plant Biotechnology Journal,2022,20(3):414-416.
[22]何崚,陈伊妮,肖海茵,等. 贡茶生产对地方生态环境影响初探[J]. 北京林业大学学报(社会科学版),2018,17(3):34-39.
[23]郑立盛. 牵挂海峡两岸的一件茶事[J]. 福建茶叶,2001(4):39-40.
[24]姚月明,巩志. 闽台乌龙茶同根同祖[J]. 农业考古,1993(2):20-21.

备注/Memo

备注/Memo:
收稿日期:2023-06-19基金项目:建瓯市茶叶科技创新专项(JO2022001)；福建省科技厅农业科技引导性项目(2021N0024)；福建农林大学科技创新专项基金项目(CXZX2020123B)；海关总署科研项目(2020HK187)作者简介:刘财国(1996-),男,江西上饶人，硕士研究生,研究方向为茶树栽培育种。(E-mail)1060737445@qq.com通讯作者:叶乃兴,(E-mail) ynxtea@126.com；于文涛,(E-mail) wtyu@foxmail.com
更新日期/Last Update: 2024-07-13