[1]朱淑斌,徐盼,周春宝,等.基于RNA-Seq技术筛选姜曲海猪子宫和卵巢发育相关基因[J].江苏农业学报,2024,(01):130-140.[doi:doi:10.3969/j.issn.1000-4440.2024.01.014]
　ZHU Shu-bin,XU Pan,ZHOU Chun-bao,et al.Screening of functional genes associated with uterus and ovary development in Jiangquhai pig based on RNA-Seq technology[J].,2024,(01):130-140.[doi:doi:10.3969/j.issn.1000-4440.2024.01.014]
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基于RNA-Seq技术筛选姜曲海猪子宫和卵巢发育相关基因()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
130-140
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2024-01-30

文章信息/Info

Title:
Screening of functional genes associated with uterus and ovary development in Jiangquhai pig based on RNA-Seq technology
作者:
朱淑斌1; 徐盼1; 周春宝1; 许琴瑟2; 吴嘉韵1
(1.江苏农牧科技职业学院,江苏泰州225300；2.姜曲海猪保种场,江苏泰州225300)
Author(s):
ZHU Shu-bin1; XU Pan1; ZHOU Chun-bao1; XU Qin-se2; WU Jia-yun1
(1.Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China；2.Jiangquhai Pig Breed Conservation Farm, Taizhou 225300, China)
关键词:
姜曲海猪; 子宫; 卵巢; 转录组测序
Keywords:
Jiangquhai pig; uterus; ovary; RNA-Seq
分类号:
S858.28
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.014
文献标志码:
A
摘要:
母猪的子宫和卵巢组织发育情况直接影响母猪的繁殖性能,进而对养猪业的经济效益造成重大影响。为探究姜曲海猪子宫和卵巢发育的分子机理,本研究选取1月龄和8月龄姜曲海猪各3头,利用转录组测序技术(RNA-Seq)对子宫和卵巢组织进行测序,通过生物信息学手段筛选差异表达基因并进行基因本体(Gene ontology, GO)和京都基因和基因组百科全书(Kyoto ncyclopedia of genes and genomes, KEGG)富集分析,再与猪数量性状基因座(Quantitative trait locus, QTL)数据库比对后鉴定出重要的候选基因。结果显示,1月龄和8月龄姜曲海猪子宫组织中有1 688个差异表达基因,表达上调和下调的基因各844个；卵巢组织中存在3 833个差异表达基因,其中2 831个表达上调,1 002个表达下调。富集分析结果显示这些差异表达基因主要显著富集于动物器官发育、组织发育和细胞分化等生物学过程。本研究在子宫和卵巢组织中分别筛选出11个和31个与发育性状有关的候选基因,其中共有的候选基因有6个,分别为LHFPL1、MTUS2、LIF、RBP4、EPHB2和TESC。本研究结果为姜曲海猪子宫和卵巢发育分子层面的研究提供了参考,也为今后姜曲海猪繁殖性状的分子改良提供了新的研究方向。
Abstract:
The development of uterus and ovary tissues can directly affect the reproductive performance in sows, which in turn has a significant impact on the economic efficiency of the pig breeding industry. To investigate the molecular mechanism of uterus and ovary development in Jiangquhai pigs, we selected three 1-month-old and three 8-month-old pigs for RNA-seq analysis of the uterus and ovary tissues. We performed statistical screening and enrichment analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to identify differentially expressed genes. Furthermore, we compared these genes with the pig quantitative trait locus (QTL) database to identify potential candidate genes. Our results showed that there were 1 688 differentially expressed genes between the uterine tissue of 1-month-old and 8-month-old Jiangquhai pigs, with 844 genes up-regulated and 844 genes down-regulated. There were 3 833 differentially expressed genes in ovarian tissue, of which 2 831 genes were up-regulated and 1 002 were down-regulated. These differentially expressed genes were significantly enriched in processes such as animal organ development, tissue development, and cell differentiation. In this study, 11 candidate genes associated with developmental traits were identified in the uterine tissue, and 31 candidate genes were identified in the ovarian tissue. Among these, six candidate genes were found to be overlapping in both tissues, namely LHFPL1, MTUS2, LIF, RBP4, EPHB2, and TESC. In conclusion, the results provide a theoretical basis for studying the molecular aspects of uterus and ovary development in Jiangquhai pigs. Additionally, it offers a new research direction for enhancing the reproductive traits of Jiangquhai pigs through molecular improvement.

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备注/Memo

备注/Memo:
收稿日期：2023-01-09基金项目：江苏农牧科技职业学院横向项目(2020321201000092)；江苏农牧科技职业学院科技服务项目(NSF2022TF02)；江苏农牧科技职业学院科技支撑项目(NSF2021TC04)；江苏省高等学校基础科学(自然科学)研究面上项目(23KJB230004)；江苏农牧科技职业学院校级科研项目(NSF2023CB01)作者简介：朱淑斌(1977-),女,黑龙江哈尔滨人,博士,教授,主要从事地方猪遗传资源保护与利用研究。(E-mail)zhushubin2003@163.com
更新日期/Last Update: 2024-03-17