[1]刘亚楠,孙枫,涂丽琴,等.本氏烟组蛋白H4与番茄斑驳花叶病毒外壳蛋白互作调控病毒侵染[J].江苏农业学报,2023,(02):344-351.[doi:doi:10.3969/j.issn.1000-4440.2023.02.006]
　LIU Ya-nan,SUN Feng,TU Li-qin,et al.Virus infection regulated by interaction between histone H4 of Nicotiana benthamiana and coat protein of tomato mottle mosaic virus[J].,2023,(02):344-351.[doi:doi:10.3969/j.issn.1000-4440.2023.02.006]
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本氏烟组蛋白H4与番茄斑驳花叶病毒外壳蛋白互作调控病毒侵染()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年02期
页码:
344-351
栏目:
植物保护
出版日期:
2023-04-30

文章信息/Info

Title:
Virus infection regulated by interaction between histone H4 of Nicotiana benthamiana and coat protein of tomato mottle mosaic virus
作者:
刘亚楠1; 孙枫2; 涂丽琴2; 高丹娜2; 李硕2; 吴淑华2; 季英华1; 2; 郭青云1
（1.青海大学农林科学院/青海省农业有害生物综合治理重点实验室/农业农村部西宁作物有害生物科学观测实验站，青海西宁810000;2.江苏省农业科学院植物保护研究所，江苏南京210014）
Author(s):
LIU Ya-nan1; SUN Feng2; TU Li-qin2; GAO Dan-na2; LI Shuo2; WU Shu-hua2; JI Ying-hua1; 2; GUO Qing-yun1
(1.Academy of Agriculture and Forestry Sciences, Qinghai University/Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province/Scientific Observing and Experimental Station of Crop Pest in Xining, Ministry of Agriculture and Rural Affairs, Xining 810000, China;2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
番茄斑驳花叶病毒; 外壳蛋白; 组蛋白; 蛋白质互作
Keywords:
tomato mottle mosaic virus; coat protein; histone; protein-protein interaction
分类号:
S436.412.1+1
DOI:
doi:10.3969/j.issn.1000-4440.2023.02.006
文献标志码:
A
摘要:
以番茄斑驳花叶病毒（Tomato mottle mosaic virus，ToMMV）外壳蛋白(Coat protein, CP)为研究对象，通过荧光素酶互补技术（Luciferase complementation imaging assay，LCI）研究ToMMV CP与本氏烟组蛋白H4在植物体内的互作情况。亚细胞共定位结果显示，ToMMV CP定位于细胞核和细胞质中，组蛋白H4定位于细胞核中，两者共定位于细胞核中。双分子荧光互补（BiFC）试验结果表明，ToMMV CP与组蛋白H4的互作位置主要在细胞核中。上述2种试验结果证明，烟草花叶病毒CP与本氏烟组蛋白H4在植物体内存在互作。采用病毒诱导的基因沉默（Virus-induced gene silencing，VIGS）技术沉默本氏烟烟草中的H4基因，对沉默H4基因的植株接种ToMMV，接种后第4 d，通过实时荧光定量PCR检测方法检测系统叶的病毒含量，发现病毒含量显著低于对照组，结果表明H4基因的沉默会影响ToMMV在植株中的复制，组蛋白H4可能是ToMMV侵染植株的本氏烟感病因子。病毒编码的CP可能与寄主组蛋白H4在细胞核内发挥作用，进而使病毒完成系统侵染。
Abstract:
The coat protein (CP) of tomato mottle mosaic virus (ToMMV) was used as the research object, and the interaction between CP of ToMMV and histone H4 of Nicotiana benthamiana was confirmed by luciferase complementation imaging assay (LCI). Subcellular localization results showed that CP of ToMMV was localized in the nucleus and cytoplasm, histone H4 was localized in the nucleus, and both were co-localized in the nucleus. In the bimolecular fluorescence complementation（BiFC) assay, the interaction sites between CP of ToMMV and histone H4 were mainly in the nucleus. The results of the above two experiments showed that there was an interaction between coat protein and histone H4 of N. benthamiana in plants. H4 was silenced in N. benthamiana by virus-induced gene silencing (VIGS). Plants with silenced H4 were inoculated with ToMMV, and the virus content in systemic leaves was detected by real-time fluorescence quantitative PCR on the fourth day after inoculation. It was found that the virus content was significantly lower than that of the control group, indicating that the silencing of H4 gene would affect the replication of ToMMV in plants, and histone H4 may be the cause of ToMMV-infected plants. The CP encoded by the virus may play a role in the nucleus with the host histone H4, thereby assisting the virus to complete the systemic infection.

参考文献/References:

[1]李月月,周文鹏,路思倩,等. 番茄斑驳花叶病毒在我国茄科作物上的发生及生物学特性[J].中国农业科学, 2020,53(3): 539-550.
[2]AMBRS S, MARTNEZ F A, IVARS P, et al. Molecular and biological characterization of an isolate of Tomato mottle mosaic virus (ToMMV) infecting tomato and other experimental hosts in eastern Spain[J]. European Journal of Plant Pathology, 2017,149(2): 261-268.
[3]金凤媚,薛俊,孙海波,等. 基于小RNA技术的天津地区番茄花叶病毒分子检测与基因组部分序列分析[J]. 华北农学报,2021,36(5)：176-183.
[4]隋雪莲.美国三种新兴蔬菜病毒病特性研究及其检测方法的建立[D].福州：福建农林大学, 2017.
[5]中国检验检疫科学研究院, 中华人民共和国天津出入境检验检疫局, 中华人民共和国甘肃出入境检验检疫局. 番茄花叶病毒检疫鉴定方法：GB/ T36771—2018[S]. 北京：国家市场监督管理总局，中国国家标准化管理委员会, 2018: 24.
[6]ISHIKAWA M, OKADA Y. Replication of tobamovirus RNA[J]. Proceedings of the Japan Academy, Series B, 2004,80(5): 215-224.
[7]杨作坤.柑橘衰退病毒与寄主蛋白互作的生物学功能及其vsiRNA靶向寄主基因的鉴定 [D].武汉：华中农业大学, 2020.
[8]涂丽琴,干射香,吴淑华,等. 瓜类褪绿黄化病毒编码的P6蛋白亚细胞定位及致病特征分析[J]. 园艺学报, 2021, 48(8): 1531-1540.
[9]范小燕,杨柳,季英华,等. 黄瓜绿斑驳花叶病毒CP基因克隆及亚细胞定位[J].江苏农业学报,2018, 34(2): 281-287.
[10]郑肖娟.传染性法氏囊病病毒感染细胞的差异蛋白质组学研究[D].杭州：浙江大学, 2007.
[11]BENDAHMANE M, SZCSI J, CHEN I, et al. Characterization of mutant tobacco mosaic virus coat protein that interferes with virus cell-to-cell movement[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002,99: 3645-3650.
[12]BANERJEE N, WANG J Y, ZAITLIN M. A single nucleotide change in the coat protein gene of tobacco mosaic virus is involved in the induction of severe chlorosis[J]. Virology, 1995, 207(1): 234-239.
[13]GALLIE D R, WALBOT V J G. RNA pseudoknot domain of tobacco mosaic virus can functionally substitute for a poly(A) tail in plant and animal cells[J]. Genes & Development,1990,4(7): 1149-1157.
[14]ASURMENDI S, BERG R H, KOO J C, et al. Coat protein regulates formation of replication complexes during tobacco mosaic virus infection[J]. Proceedings of the National Academy of Sciences of the United States of America,2004,101(5): 1415-1420.
[15]何晓玥. 核盘菌GATA转录因子SsAREA和SsSRE的功能研究[D].长春：吉林大学, 2021.
[16]OKADA T, ENDO M, SINGH M B, et al. Analysis of the histone H3 gene family in Arabidopsis and identification of the male-gamete-specific variant AtMGH3[J]. Plant J,2005,44(4): 557-568.
[17]YANG H, YANG N, WANG T. Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii[J]. Plant J, 2016,85(5): 660-674.
[18]TALASZ H, LINDNER H H, SARG B, et al. Histone H4-lysine 20 monomethylation is increased in promoter and coding regions of active genes and correlates with hyperacetylation[J]. J Biol Chem, 2005,280(46): 38814-38822.
[19]李霞. 水稻OsH4a基因的克隆与抗逆性分析[D].雅安：四川农业大学, 2018.
[20]李咏欣. 基于多肽化学反应的荧光探针的设计、合成与生物应用[D].兰州：兰州大学, 2021.
[21]WANG Z, CASAS-MOLLANO J A, XU J P, et al. Osmotic stress induces phosphorylation of histone H3 at threonine 3 in pericentromeric regions of Arabidopsis thaliana[J]. PNAS, 2015, 112(27): 8487-8492.
[22]MESSNER S, HOTTIGER M O. Histone ADP-ribosylation in DNA repair, replication and transcription[J]. Trends Cell Biol, 2011, 21(9): 534-542.
[23]WON J, KIM T K. Histone modifications and transcription factor binding on chromatin ChIP-PCR assays[J]. Methods Mol Biol, 2006,325: 273-283.
[24]李阳,谢理,祝建烜,等. 水稻条纹病毒p2与本氏烟柯浩体蛋白互作[J]. 病毒学报,2021,37(6): 1476-1483.
[25]徐美玲,孙健英,李宗芸. 植物着丝粒组成和功能的研究进展[J].生命科学,2022,34(3): 285-293.
[26]徐东亮,刘永,张炜,等. 核因子κB诱骗剂处理的树突状细胞延长小鼠移植心脏存活时间[J].中华器官移植杂志,2007,28(9): 525-529.
[27]郭嘉媛,洪永河,黄健强,等. 稻瘟病菌无毒效应因子Avr-PikD与水稻蛋白OsDjA9的互作鉴定[J]. 福建农业学报,2022, 37(5): 668-674.
[28]MAKAROV V V, KALININA N O. Structure and noncanonical activities of coat proteins of helical plant viruses[J]. Biochemistry (Mosc), 2016,81(1): 1-18.
[29]苗艳梅,赵敏. 马铃薯Y病毒属病毒外壳蛋白功能[J].黑龙江农业科学, 2019(3): 165-168.
[30]TAKAMATSU N, ISHIKAWA M, MESHI T, et al. Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA[J]. Embo J, 1987,6(2): 307-311.
[31]HAMEED A, TAHIR M N, ASAD S, et al. RNAi-mediated simultaneous resistance against three RNA viruses in potato[J]. Mol Biotechnol, 2017,59(2/3): 73-83.
[32]王继伟,雷新云,严衍录,等. 感染烟草花叶病毒(TMV)的烟叶在显症前的荧光光谱变化[J]. 植物保护学报，1995(3): 269-274.
[33]ABBINK T E M, PEART J R, MOS T N M, et al. Silencing of a gene encoding a protein component of the oxygen-evolving complex of photosystem Ⅱ enhances virus replication in plants[J]. Virology, 2002, 295(2): 307-319.
[34]谢联辉,林奇英. 植物病毒学[M]. 北京：中国农业出版社,2011.
[35]ZHU S F, GAO F, CAO X S, et al. The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms[J]. Plant Physiol, 2005,139(4): 1935-1945.
[36]LI Y, WU M Y, SONG H H, et al. Identification of a tobacco protein interacting with tomato mosaic virus coat protein and facilitating long-distance movement of virus[J]. Arch Virol, 2005,150(10): 1993-2008.
[37]郑海刚. 黄瓜绿斑驳花叶病毒外壳蛋白与寄主烟草蛋白间的互作[D].福州：福建农林大学, 2011.
[38]王宏鑫,王萌,张煜伟,等. 胞外组蛋白与急性器官损伤的关系[J].武警医学,2019, 30(5): 441-444.
[39]顾欢. 大豆和苜蓿水平转移基因的鉴定与功能分析[D].南京：南京农业大学, 2017.
[40]TESSARZ P, KOUZARIDES T. Histone core modifications regulating nucleosome structure and dynamics[J]. Nat Rev Mol Cell Biol, 2014,15(11): 703-708.
[41]李霞,张玉军. 组蛋白H4的共价修饰在表观遗传调控中的作用研究进展[J]. 山东医药, 2013, 53(15): 91-93.
[42]LIU X Y, ZHANG X H, ZHANG Z J. Point mutation of H3/H4 histones affects acetic acid tolerance in Saccharomyces cerevisiae[J]. Journal of Biotechnology, 2014,187: 116-123.
[43]KIM W H, LATRASSE D, SERVET C, et al. Arabidopsis histone deacetylase HDA9 regulates flowering time through repression of AGL19[J]. Biochemical and Biophysical Research Communications, 2013, 432(2): 394-398.
[44]XIAO J, ZHANG H, XING L J, et al. Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis[J]. Journal of Plant Physiology, 2013, 170(4): 444-451.
[45]苗蒙. RNA病毒与宿主蛋白相互作用机制的研究[D].武汉：武汉大学, 2019.

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备注/Memo

备注/Memo:
收稿日期：2023-02-10 基金项目：国家重点研发计划项目（2022YFD1401202）；国家自然科学基金项目(32072506)；江苏省农业科技自主创新基金项目[CX(21)1011]；现代农业产业技术体系项目(CARS-24-C-01)；沿海集团揭榜挂帅项目（2022YHTDJB03）；高端外国专家引进计划项目（G2022014073L）；江苏省农业科学院科学仪器开放共享自主研究课题[GX(22)1001] 作者简介：刘亚楠（1997-），女，河南驻马店人，硕士研究生，研究方向为作物病毒，(E-mail)2228239761@qq.com。孙枫为共同第一作者。通讯作者：郭青云，（E-mail）Guoqingyunqh@163.com；季英华，(E-mail）jiyinghua@jaas.ac.cn
更新日期/Last Update: 2023-05-12