[1]姜玫宏,葛金山,程金龙,等.菌酶协同异步发酵中草药对断奶仔猪生长、腹泻、免疫和抗氧化性能的影响[J].江苏农业学报,2023,(01):126-133.[doi:doi:10.3969/j.issn.1000-4440.2023.01.015]
　JIANG Mei-hong,GE Jin-shan,CHENG Jin-long,et al.Effects of bacteria-enzymes synergistic asynchronous fermentation of Chinese herbal medicine on growth, diarrhea, immunity and antioxidant of weaned piglets[J].,2023,(01):126-133.[doi:doi:10.3969/j.issn.1000-4440.2023.01.015]
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菌酶协同异步发酵中草药对断奶仔猪生长、腹泻、免疫和抗氧化性能的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
126-133
栏目:
畜牧兽医·水产养殖
出版日期:
2023-02-28

文章信息/Info

Title:
Effects of bacteria-enzymes synergistic asynchronous fermentation of Chinese herbal medicine on growth, diarrhea, immunity and antioxidant of weaned piglets
作者:
姜玫宏1; 葛金山2; 程金龙3; 彭宇晗1; 姜淑贞4; 朱元召1; 5
（1.安徽科技学院，安徽凤阳233100；2.山东众成饲料科技有限公司，山东肥城271600；3.江苏优仕生物科技发展有限公司，江苏宿迁223800；4.山东农业大学，山东泰安271000；5.河北和利美生物科技有限公司，河北青县062650）
Author(s):
JIANG Mei-hong1; GE Jin-shan2; CHENG Jin-long3; PENG Yu-han1; JIANG Shu-zhen4; ZHU Yuan-zhao1; 5
(1.Anhui Science and Technology University, Fengyang 233100, China;2.Shandong Zhongcheng Feed Technology Co., Ltd., Feicheng 271600, China;3.Jiangsu Unison Biotechnology Development Co., Ltd., Suqian 223800, China;4.Shandong Agricultural University, Tai’an 271000, China;5.Hebei Heliomy Biotechnology Co., Ltd., Qingxian 062650, China)
关键词:
菌酶协同; 异步发酵; 复方中草药; 断奶仔猪; 生长; 免疫
Keywords:
bacteria-enzymes synergy; asynchronous fermentation; compound Chinese herbal medicine; weaned piglets; growth; immunity
分类号:
S816.7
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.015
文献标志码:
A
摘要:
以3种加工处理的中草药饲喂断奶仔猪，研究其对断奶仔猪生长、腹泻、免疫、血清脂与酶及抗氧化等指标的影响。120头28±2日龄杜长大断奶仔猪分4组，3组中草药处理组饲粮分别含2%粉碎复方中草药、2%复方中草药提取液和2%发酵复方中草药，对照组饲粮不含中草药，试验期为28 d。结果表明，与对照组相比，粉碎中草药组断奶仔猪料质量比(F/G)、总蛋白(TP)含量、球蛋白(GLB)含量、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)含量均有显著差异(P<0.05)；中草药提取液组平均日增质量(ADG)、F/G、试验全期腹泻指数、免疫球蛋白G(IgG)含量、白蛋白(ALB)含量、GLB含量、TP含量、谷丙转氨酶(ALT)含量、谷草转氨酶(AST)含量、碱性磷酸酶(ALP)含量、谷胱甘肽过氧化物酶(GSH-PX)含量、总抗氧化能力(T-AOC)、SOD含量和丙二醛(MDA)含量均有显著差异(P<0.05)；发酵中草药组ADG、平均日采食量(ADFI)、F/G、腹泻指数、血清IgG含量、免疫球蛋白A(IgA)含量、免疫球蛋白M(IgM)含量、ALB含量、GLB含量、TP含量、总胆固醇(TC)含量、甘油三酯(TG)含量、ALT含量、AST含量、ALP含量、GSH-PX含量、过氧化氢酶(CAT)含量、T-AOC、SOD含量和MDA含量均有显著变化(P<0.05)。由此可见，与对照组及粉碎中草药组、中草药提取液组相比，发酵中草药组中草药经菌酶协同先好氧后厌氧异步发酵能较好地改善断奶仔猪生长性能，具有抗腹泻、增强机体免疫和抗氧化功能，提示与中草药功效增强和发酵代谢产物作用叠加有关。
Abstract:
The effects of three processed Chinese herbal medicines on growth, diarrhea, immunity, serum lipids and enzymes and antioxidant of weaned piglets were studied. One hundred and twenty Duroc-Landrace-Yorkshire weaned piglets at 28±2 days of age were divided into four groups. The control group was fed with no Chinese herbal medicine, and the crushed Chinese herbal medicine group, Chinese herbal extract group and fermented Chinese herbal medicine group were fed with 2% crushed Chinese herbal medicine, 2% compound Chinese herbal medicine extract and 2% fermented Chinese herbal medicine, respectively. The experiment lasted for 28 days. Compared with the control group, the crushed Chinese herbal medicine group was significantly different in the ratio of feed and gain (F/G), total protein (TP) content, globulin (GLB) content, total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) content (P<0.05), the Chinese herbal medicine extract group was significantly different in average daily gain (ADG), F/G, diarrhea index, immunoglobulin G (IgG) content, albumin (ALB) content, GLB content, TP content, alanine aminotransferase (ALT) content, aspartate aminotransferase (AST) content, alkaline phosphatase (ALP) content, glutathione peroxidase (GSH-PX) content, T-AOC, SOD content and malonaldehyde (MDA) content (P<0.05), and the fermented Chinese herbal medicine group was significantly different in ADG, average daily feed intake (ADFI), F/G, diarrhea index, IgG content, immunoglobulin A (IgA) content, immunoglobulin M (IgM) content, ALB content, GLB content, TP content, total cholesterol (TC) content, triglyceride (TG) content, ALT content, AST content, ALP content, GSH-PX content, catalase (CAT) content, T-AOC, SOD content and MDA content (P<0.05). Compared with the control group, crushed Chinese herbal medicine group and Chinese herbal medicine extract group, the fermented Chinese herbal medicine group can improve the growth performance of weaned piglets, enhance immune and antioxidant functions, and has anti-diarrhea by aerobic and anaerobic asynchronous fermentation with bacteria and enzymes, suggesting that it is related to the superposition between the enhanced effect of Chinese herbal medicine and fermentation metabolites.

参考文献/References:

[1]赵军宁. 中药复方适度调节原理与中药复方新药转化中的药理学问题[J].中国中药杂志,2017,42(5):836-843.
[2]胡念青. 中草药复方对保育仔猪脾脏抗氧化和抗炎症功能的影响[D].南昌:江西农业大学,2020.
[3]李琳倩,张云龙,刘日亮,等. 中草药添加剂对后备母猪生长性能、发情率、血清指标和肠道微生物的影响[J].动物营养学报,2020,32(6):2586-2600.
[4]李秋月,林连兵,杨雪娇,等. 微生物发酵中草药的研究现状[J].微生物学通报,2021,48(6):2232-2244.
[5]LI L, WANG L, FAN W X, et al. The application of fermentation technology in traditional Chinese medicine:a review[J]. The American Journal of Chinese Medicine,2020,48(4):899-921.
[6]WANG R, CAI W T, WANG X L, et al. Progress in Chinese medicine-probiotics compound microecological preparations for livestock and poultry[J]. Chinese Journal of Biotechnology, 2019, 35(6): 972-987.
[7]刘波,张鹏翼,孟祥璟,等. 益生菌发酵中药方法概述及其应用研究进展[J].中国现代中药, 2020, 22(10): 1741-1750.
[8]DONG W W, XUAN F L, ZHANG W L, et al. Comparative analysis of the rats’ gut microbiota composition in animals with different ginsenosides metabolizing activity[J]. Journal of Agricultural and Food Chemistry, 2017, 65(2):327-337.
[9]GUEVARRA R B, HONG S H, CHO J H, et al. The dynamics of the piglet gut microbiome duringthe weaning transition in association with health and nutrition[J]. Journal of Animal Science and Biotechnology,2018,9(1):1-9.
[10]吴东,计徐,周芬,等. 发酵中草药添加剂对仔猪生长性能、血清免疫指标及肠道菌群的影响[J].养猪, 2021(6):11-14.
[11]郭建来,魏红芳,吴金梅,等. 发酵复方党参对断奶仔猪生长性能和血清免疫球蛋白含量的影响[J].现代牧业, 2020,4(3):10-13.
[12]ZHAO P Y, LIN H L, LEI Y, et al. Effect of fermented medicinal plants on growthperformance nutrient digestibility fecalnoxious gas emissions and diarrhea score inweanling pigs[J]. Journal of the Science of Food and Agriculture,2016, 96(4):1269-1274.
[13]邹志恒,季华员,陈小连,等. 复方中草药发酵制剂对断奶仔猪生长性能、免疫功能和血清生化指标的影响[J].中国畜牧兽医,2019,46(4):1038-1044.
[14]方磊涵,王中华,王留. 中药复方发酵剂对断奶仔猪生长性能、血清抗氧化活性及肠道菌群的影响[J].饲料研究, 2019,42(8):11-15.
[15]徐博成,路则庆,邓近平,等. 发酵饲料对断奶仔猪和生长育肥猪生长性能影响的Meta分析[J]. 饲料工业, 2018, 39(24):40-48.
[16]史自涛,姚焰础,江山,等. 粪肠球菌替代抗生素对断奶仔猪生长性能、腹泻率、血液生化指标和免疫器官的影响[J].动物营养学报,2015,27(6):1832-1840.
[17]张玉千. 发酵中药渣对断奶仔猪生长性能、肠道菌群及免疫功能的影响[J].饲料研究,2020,43(11):47-49.
[18]侯海峰,李茜. 发酵中草药渣对断奶仔猪生长性能及肠道健康的影响[J].中国饲料,2017(21):21-24.
[19]高鹏辉,王宏展,邓晓敏,等. 发酵复方中草药的不同添加量对断奶仔猪的影响[J].饲料工业,2015,36(19):30-33.
[20]李燕婧,钟正贤,陈学芬,等. 长柱十大功劳叶与阔叶十大功劳水提物药理作用比较[J].中医药导报, 2010, 16(9): 92-93,96.
[21]ZHAO S S, MA D X, ZHU Y, et al. Antidiarrheal effect of bioactivity-guided fractions and bioactive components of pomegranate (Punica granatum L.) peels[J]. Neurogastroenterology and Motility,2018,30(7):1-10.
[22]库尔班·吾斯曼，穆赫塔尔·伊米尔艾山，帕提古丽·伊米尔艾山. 马齿苋子中多糖的提取分离及抑菌活性研究[J]. 食品研究与开发，2009，30（7）：28-30.
[23]NIHO Y, MITSUNAGA K, KOIKE K, et al. Studies on the gastric antiulcer components from the woods of Picrasma quassioides(Simaroubaceae)[J]. Natural Medicines,1994,48(2):116-121.
[24]冯津津. 马齿苋的化学成分及药理作用研究进展[J].云南中医中药杂志,2013,34(7):66-68.
[25]赵文娜,张新新,谢人明,等. 苦木化学成分和药理作用研究进展[J].中药材,2011,34(7):1149-1152.
[26]WANG Y B, XIE Q H, SUN S, et al. Probiotics-fermented Massa Medicata Fermentata ameliorates weaning stress in piglets related to improving intestinal homeostasis[J]. Applied Microbiology and Biotechnology, 2018, 102(24):10713-10727.
[27]LARROSA M, TOMAS-BARBERAN F A, ESPIN J C. The dietary hydrolysable tannin punicalagin releases ellagic acid that induces apoptosis in human colon adenocarcinoma Caco-2 cells by using the mitochondrial pathway[J]. Journal of Nutritional Biochemistry, 2006, 17(9): 611-625.
[28]黄伟煌,吴德峰,郑真珠,等. 中草药降低生态兔胆固醇和血清酶生化指标的试验研究[J].中国养兔,2013(1):4-7.
[29]骆雪,俞伟辉. 复方中草药添加剂对断奶仔猪生长性能、血清生化指标及免疫指标的影响[J].饲料研究, 2021, 18:40-43.
[30]王利军,赵文文,么恩悦,等. 发酵北苍术对断奶仔猪血清免疫和生化指标的影响[J].东北农业大学学报, 2017, 48(10):35-41.
[31]何维敏,李筱雯,况世昌,等. 发酵中药微生态制剂对断奶仔猪生长性能、血清生化指标和抗氧化能力的影响[J].湖北农业科学,2021,60(18):121-124.
[32]安琦,曹亚彬,牛彦波,等. 发酵黄芩药渣对仔猪生长性能、氧化应激及免疫功能的影响[J].饲料工业, 2021, 42(6):17-21.
[33]贾谏,陈庄,陈中健,等. 鬼针草属植物化学成分、药理作用及其在畜牧生产上的研究进展[J].中国畜牧兽医生,2013,40(8):199-203.
[34]李佳,陈皓玉,张文慧,等. 石榴皮中酚类化合物药理作用研究进展[J].粮食与油脂,2020,33(12):4-6.
[35]HE J M, MU Q, WANG C L, et al. The medicinal uses of the genus Mahonia in traditional Chinese medicine: an ethnopharmacological, phytochemical and pharmacological review[J]. Journal of Ethnopharmacol, 2015, 175: 668-683.
[36]ZHAI X C, ZHU C P, ZHANG Y, et al. Chemical characteristics, antioxidant capacities and hepatoprotection of polysaccharides from pomegranate pee[J]. Carbohydrate Polymers, 2018, 202: 461-469.
[37]MOHAMED A D, AHMED E A, SALEH A Q, et al. Antioxidant effect of purslane(Pertulaca oleracea)andits mechanism of action[J]. Journal of Medicinal Plants Research, 2011, 5(9): 1589-1563.
[38]戴燊. 中草药混菌发酵生产新型生物制剂的研究[D].合肥:安徽农业大学,2013.

备注/Memo

备注/Memo:
收稿日期：2022-03-16基金项目：山东省中央引导地方科技发展资金项目(YDZX20203700003336)；泰安市科技创新重大专项(2021ZDZX033)；宿迁市科技计划项目（L202102）作者简介：姜玫宏（1996-），女，山东海阳人，硕士研究生，研究方向为动物营养与饲料。（E-mail）759596326@qq.com通讯作者：朱元召，（E-mail）zhuyuanzhao111@163.com
更新日期/Last Update: 2023-03-21