[1]邵颖,肖福泉,王丽丽,等.血清4型禽腺病毒POCT荧光微球免疫层析检测方法的建立[J].江苏农业学报,2022,38(05):1298-1305.[doi:doi:10.3969/j.issn.1000-4440.2022.05.017]
　SHAO Ying,XIAO Fu-quan,WANG Li-li,et al.Establishment of POCT fluorescent microsphere immunochromatography method for detection of fowl adenovirus type 4[J].,2022,38(05):1298-1305.[doi:doi:10.3969/j.issn.1000-4440.2022.05.017]
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血清4型禽腺病毒POCT荧光微球免疫层析检测方法的建立()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年05期
页码:
1298-1305
栏目:
畜牧兽医·水产养殖
出版日期:
2022-10-31

文章信息/Info

Title:
Establishment of POCT fluorescent microsphere immunochromatography method for detection of fowl adenovirus type 4
作者:
邵颖1; 2; 肖福泉1; 2; 王丽丽1; 2; 龚柳菲1; 2; 宋祥军1; 2; 涂健1; 2; 祁克宗1; 2
（1.兽医病理生物学与疫病防控安徽省重点实验室，安徽合肥230036；2.安徽省动物性食品质量与生物安全工程实验室，安徽合肥230036）
Author(s):
SHAO Ying1; 2; XIAO Fu-quan1; 2; WANG Li-li1; 2; GONG Liu-fei1; 2; SONG Xiang-jun1; 2; TU Jian 1; 2; QI Ke-zong1; 2
(1.Anhui Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei 230036, China；2.Anhui Province Animal Food Quality and Biosafety Engineering Laboratory, Hefei 230036, China)
关键词:
FAdV-4; hexon基因; 原核表达; 多克隆抗体; 荧光微球免疫层析
Keywords:
FAdV-4; hexon; prokaryotic expression; polyclonal antibody; fluorescent microphere immunochromatography
分类号:
S858.326.5
DOI:
doi:10.3969/j.issn.1000-4440.2022.05.017
文献标志码:
A
摘要:
为了迅速且便利地判定血清４型禽腺病毒（FAdV-4），设计引物进行扩增，构建重组载体，原核表达的融合蛋白乳化后动物免疫得到多克隆抗体，用Western Blot检测，最后建立即时检验（POCT）荧光微球免疫层析试纸条，并进行初步应用。用POCT荧光微球免疫层析试纸条检测不同浓度的FAdV-4病毒液及临床阳性样本，结果表明，用POCT荧光微球免疫层析试纸条15 min可以检测出结果，且检测出阳性，与临床诊断结果相符。建立的POCT荧光微球免疫层析检测FAdV-4的方法，特异性良好，操作简单且便于携带，检测效率高，有利于基层兽医临床检测。
Abstract:
To quickly and conveniently determine fowl adenovirus serotype 4 (FAdV-4), primers were designed for amplification, and the recombinant vector was constructed. After the fusion protein was emulsified, the polyclonal antibody was obtained by animal immunization. Western blot was used to detect, and point-of-care testing (POCT) fluorescent microsphere immunochromatographic strip was established and preliminary applized. The POCT fluorescent microsphere immunochromatographic strip was used to detect different contents of FAdV-4 and clinical positive sample. The results could be detected by poa fluorescent microsphere immunochromatographic strip for 15 minutes, and the positive results were consistent with the clinical diagnostic results. The POCT fluorescence immunochromatography assay has good specificity, simple operation and portability, high detection efficiency, so it is conducive to clinical detection of primary veterinarians.

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期：2021-10-26基金项目：国家自然科学基金面上项目（31972642）；安徽省高校协同创新项目（GXXT-2019-035）；安徽省大学生创新创业训练计划项目(202110364043、202110364027)作者简介：邵颖（1989-），女，安徽亳州人，博士研究生，主要从事动物疫病防控研究，(E-mail)shaoying@ahau.edu.cn通讯作者：祁克宗，(E-mail)qkz@ahau.edu.cn
更新日期/Last Update: 2022-11-07