[1]吴雅琼,张春红,杨海燕,等.基于代谢组学分析黑莓、黑树莓果实代谢物的差异[J].江苏农业学报,2022,38(03):790-797.[doi:doi:10.3969/j.issn.1000-4440.2022.03.026]
　WU Ya-qiong,ZHANG Chun-hong,YANG Hai-yan,et al.Metabolomics analysis of differentially expressed metabolites in blackberry and black raspberry fruits[J].,2022,38(03):790-797.[doi:doi:10.3969/j.issn.1000-4440.2022.03.026]
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基于代谢组学分析黑莓、黑树莓果实代谢物的差异()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
790-797
栏目:
园艺
出版日期:
2022-06-30

文章信息/Info

Title:
Metabolomics analysis of differentially expressed metabolites in blackberry and black raspberry fruits
作者:
吴雅琼1; 张春红1; 杨海燕1; 闾连飞1; 李维林2; 吴文龙1
（1.江苏省中国科学院植物研究所，江苏南京210014；2.南京林业大学南方现代林业协同创新中心，江苏南京210037）
Author(s):
WU Ya-qiong1; ZHANG Chun-hong1; YANG Hai-yan1; LYU Lian-fei1; LI Wei-lin2; WU Wen-long1
(1.Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;2.Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China)
关键词:
黑莓; 黑树莓; 黄酮; 差异代谢物; 合成通路
Keywords:
blackberry; black raspberry; flavonoid; differentially expressed metabolites; biosynthesis pathway
分类号:
S663.9
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.026
文献标志码:
A
摘要:
为了探究黑莓、黑树莓成熟果实中主要代谢物的差异，利用液相色谱-质谱技术对其进行比较分析。结果表明，在正离子、负离子模式下分别存在1 288个、971个具有显著表达差异的代谢物，通过KEGG数据平台分析发现，代谢通路是富集差异表达代谢物数量最多的（共104个），其次是次生代谢物合成通路（有65个差异表达代谢物）。此外，黄酮和黄酮醇生物合成通路也是主要差异代谢物显著富集的代谢途径之一，包含11个差异代谢物。而在花色苷合成通路上，与黑莓果实相比，黑树莓果实中有6个代谢物表达量下调，而矢车菊素-3-O-葡萄糖苷代谢物表达量上调。在异黄酮合成通路中，与黑树莓果实相比，黑莓果实中的(S)-柚皮素相对表达量增加了2.7倍。研究结果为更好地了解黑树莓、黑莓果实代谢物的异同和高效利用悬钩子属果实中黄酮、花色苷等次生代谢物提供理论依据。
Abstract:
To explore the differences of main metabolites between mature fruits of black raspberry and blackberry, liquid chromatography-mass spectrometry (LC-MS) was used to compare and analyze. The results showed that there were 1 288 and 971 metabolites with significant differences in positive and negative ion modes, respectively. Through the analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) data platform, it was found that metabolic pathways were the most abundant (104 differentially expressed metabolites), followed by secondary biosynthesis (65 differentially expressed metabolites). In addition, flavone and flavonol biosynthesis was also one of the major metabolic pathways with significant enrichment, including 11 differentially expressed metabolites. In the anthocyanin biosynthesis pathway, compared with the mature fruit of blackberry, there were six differentially expressed metabolites down-regulated in the mature fruit of black raspberry. The expression level of cyanidin-3-o-glucoside metabolites was up-regulated in the black raspberry. In the isoflavonoid biosynthesis pathway, the relative expression of (S)-naringenin in blackberry fruit was 3.7 times as much as that in black raspberry. The results can provide a theoretical basis for better understanding the similarities and differences of metabolites in black raspberry and blackberry fruits, and efficient utilization of secondary metabolites such as flavonoids and anthocyanins in Rubus fruits.

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备注/Memo

备注/Memo:
收稿日期：2021-10-17基金项目：国家自然科学基金项目（32101566）；江苏省自然科学基金青年基金项目（BK20210165）；江苏省中国科学院植物研究所博士人才科研启动基金项目（JIBTF202105）作者简介：吴雅琼（1992-），女，江苏南京人，博士，助理研究员，主要从事小浆果遗传育种、果实营养品质和基因组学方面的研究。(E-mail)ya_qiong@126.com通讯作者：李维林，(E-mail)wlli@njfu.edu.cn；吴文龙，(E-mail)1964wwl@163.com
更新日期/Last Update: 2022-07-07