[1]裴徐梨,胡文婷,荆赞革,等.中国南瓜蔗糖转化酶（INV）基因的鉴定及进化和表达分析[J].江苏农业学报,2022,38(03):782-789.[doi:doi:10.3969/j.issn.1000-4440.2022.03.025]
　PEI Xu-li,HU Wen-ting,JING Zan-ge,et al.Analysis on identification, evolution and expression of sucrose invertase gene (INV) in Cucurbita moschata[J].,2022,38(03):782-789.[doi:doi:10.3969/j.issn.1000-4440.2022.03.025]
点击复制

中国南瓜蔗糖转化酶（INV）基因的鉴定及进化和表达分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年03期
页码:
782-789
栏目:
园艺
出版日期:
2022-06-30

文章信息/Info

Title:
Analysis on identification, evolution and expression of sucrose invertase gene (INV) in Cucurbita moschata
作者:
裴徐梨1; 胡文婷1; 荆赞革1; 焦鹏1; 朱丽瑞1; 宋立晓2; 严继勇3
（1.昆明学院农学与生命科学学院，云南昆明650214；2.江苏省农业科学院农业资源与环境研究所，江苏南京210014;3.江苏省农业科学院蔬菜研究所，江苏南京210014）
Author(s):
PEI Xu-li1; HU Wen-ting1; JING Zan-ge1; JIAO Peng1; ZHU Li-rui1; SONG Li-xiao2; YAN Ji-yong3
(1.College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China;2.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
中国南瓜; INV基因; 序列特征; 进化分析
Keywords:
Cucurbita moschata; invertase (INV) gene; sequence characteristics; evolutionary analysis
分类号:
S642.1
DOI:
doi:10.3969/j.issn.1000-4440.2022.03.025
文献标志码:
A
摘要:
蔗糖转化酶（INV）编码基因广泛参与植物的生长发育，在果实成熟期可影响果实品质。本研究利用生物信息学手段对中国南瓜INV基因家族进行鉴定和分析。结果表明，从中国南瓜中鉴定出18个INV基因家族成员，其功能分为酸性转化酶和中性/碱性转化酶两大类。该家族成员在11条染色体上不均匀分布，且仅有1对串联重复基因。表达模式分析结果显示，CmoCh06G004890.1在根中的相对表达量高于其他INV基因家族成员，CmoCh01G010160.1和CmoCh06G004890.1在果实中的相对表达量较高。葫芦科作物INV系统进化分析结果表明，中国南瓜、印度南瓜和美洲南瓜的大多数INV基因均聚为一簇，具有较近的亲缘关系。本研究结果为进一步分析中国南瓜INV基因的生物学功能以及提高中国南瓜品质提供了一定的理论依据。
Abstract:
Invertase (INV) encoding genes are widely involved in plant growth and development, and can affect fruit quality during ripening stage. In this study, INV gene family of Cucurbita moschata was identified and analyzed by bioinformatic methods. The results showed that, 18 members of INV gene family were identified from C. moschata, and their corresponding functions were encoding acid INV and neutral/alkaline INV. The members of INV gene family distributed unevenly on 11 chromosomes and there only existed one pair of tandem duplicated genes. Analysis of expression pattern showed that, the relative expression level of CmoCh06G004890.1 in roots of C. moschata was higher than other members of INV gene family. The relative expression levels of CmoCh01G010160.1 and CmoCh06G004890.1 were relatively high in fruits of C. moschata. Results of phylogenetic analysis of INV genes in Cucurbitaceae crops showed that, Most of the INV genes from C.moschata, C.maxima and C. pepo gathered into one cluster and had close genetic relationships. The results can provide theoretical basis for further analyzing of the biological functions of INV genes and improving fruit quality of C. moschata.

参考文献/References:

[1]俞锞,李志邈,万红建,等. 高等植物蔗糖转化酶功能的研究进展 [J]. 安徽农业科学, 2013, 41(33): 12815-12818.
[2]姜立智,林长发,梁宗锁,等. 水稻蔗糖转化酶基因的克隆及其功能的初步探讨 [J]. 复旦学报(自然科学版), 2003，42(4): 588-592.
[3]高媛媛,杨郁文,张保龙,等. 番茄中性/碱性蔗糖转化酶基因的电子克隆、分析及表达载体的构建[J].江苏农业科学, 2009，6: 36-38.
[4]魏华伟,柴松琳,胡克玲,等. 辣椒酸性蔗糖转化酶基因家族鉴定及表达 [J]. 分子植物育种, 2019, 17(15): 4900-4907.
[5]占雷雷,朱国鹏,刘永华. 4种蔗糖分解酶在甘薯块根品质形成中的作用 [J]. 热带作物学报, 2019, 40(9): 1723-1728.
[6]YU X, WANG X, ZHANG W, et al. Antisense suppression of an acid INVertase gene (MAI1) in muskmelon alters plant growth and fruit development [J]. J Exp Bot, 2008, 59(11): 2969-2977.
[7]JANG J C, LEON P, ZHOU L, et al. Hexokinase as a sugar sensor in higher plants [J]. Plant Cell, 1997, 9(1): 5-19.
[8]TANG X, SU T, HAN M, et al. Suppression of extracellular INVertase inhibitor gene expression improves seed weight in soybean (Glycine max) [J]. J Exp Bot, 2017, 68(3): 469-482.
[9]QIAN W, YUE C, WANG Y, et al. Identification of the INVertase gene family (INVs) in tea plant and their expression analysis under abiotic stress [J]. Plant Cell Rep, 2016, 35(11): 2269-2283.
[10]FINN R D, BATEMAN A, CLEMENTS J, et al. Pfam: the protein families database [J]. Nucleic Acids Res, 2014, 42: 222-230.
[11]朱冉冉,吉雪花,张中荣,等. 辣椒超氧化物歧化酶基因家族的生物信息学分析 [J]. 石河子大学学报(自然科学版), 2020, 38(6): 712-717.
[12]苗小荣,牛俊奇,莫昭展,等. 铁皮石斛中性/碱性转化酶(DoNI2)基因的克隆和表达分析 [J]. 中草药, 2018, 49(15): 208-215.
[13]阳江华,龙翔宇,邹智,等. 橡胶树中碱性转化酶基因HbNIN8的克隆与表达分析 [J]. 热带作物学报, 2018, 39(12): 2421-2426.
[14]韩玉慧,陈琴,张会,等. 棉花蔗糖转化酶基因家族的生物信息学分析 [J]. 中国农业大学学报, 2018, 23(11): 15-30.
[15]王海波,辛胡,刘潮,等. 小桐子转化酶基因家族的生物信息学分析及表达特性[J].生物技术通报, 2017, 33(9): 172-178.
[16]吴晓慧,耿梦婷,范洁,等. 木薯转化酶抑制子MeINH3基因的克隆及生物信息学分析 [J]. 热带作物学报, 2014, 35(9): 1715-1720.
[17]帅良,廖玲燕,韩冬梅,等. 龙眼中性转化酶基因(DlNI)的克隆及分析 [J]. 西南农业学报, 2017, 30(10): 48-55.
[18]赵智中. 柑橘果实糖积累的生理基础研究 [D]. 杭州：浙江大学, 2001.
[19]LIU Y F, QI H Y, BAI C M, et al. Grafting helps improve photosynthesis and carbohydrate metabolism in leaves of muskmelon [J]. International Journal of Biological Sciences, 2011, 7(8): 1161-1170.
[20]张琼琼. 番茄中性/碱性转化酶SlCIN3e基因功能研究 [D]. 沈阳：沈阳农业大学, 2019.
[21]王滕旭,李正国,杨迎伍,等. 哈姆林甜橙蔗糖合酶Ⅰ和酸性转化酶基因表达与果实糖积累的关系 [J].热带作物学报, 2010, 31(5): 745-749.
[22]TIAN H, KONG Q G, FENG Y Q, et al. Cloning and characterization of a soluble acid INVertase-encoding gene from muskmelon [J]. Molecular Biology Reports, 2009, 36(4): 611-617.
[23]郭利军,邓会栋,冯学杰,等. 芒果中性/碱性转化酶MiNI基因的克隆与表达分析 [J]. 热带作物学报, 2020, 41(9): 1741-1747.

备注/Memo

备注/Memo:
收稿日期：2021-08-07基金项目：昆明学院引进人才科研项目 (YJL19009)；云南省教育厅科学研究基金项目 (2021Y717、2021Y721)作者简介：裴徐梨（1990-），女，云南个旧人，博士，讲师，研究方向为蔬菜分子生物学。（E-mail）xuliP1990@163.com通讯作者：宋立晓，（E-mail）56785633@qq.com
更新日期/Last Update: 2022-07-07