[1]谢佳彤,孙丽丹,陈晓曼,等.麻风树JcWRI1基因克隆及功能分析[J].江苏农业学报,2022,38(02):334-342.[doi:doi:10.3969/j.issn.1000-4440.2022.02.006]
　XIE Jia-tong,SUN Li-dan,CHEN Xiao-man,et al.Cloning and functional analysis of JcWRI1 gene from physic nut[J].,2022,38(02):334-342.[doi:doi:10.3969/j.issn.1000-4440.2022.02.006]
点击复制

麻风树JcWRI1基因克隆及功能分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
334-342
栏目:
遗传育种·生理生化
出版日期:
2022-04-30

文章信息/Info

Title:
Cloning and functional analysis of JcWRI1 gene from physic nut
作者:
谢佳彤; 孙丽丹; 陈晓曼; 方彤彤; 张苗苗; 赵雨凡; 杨冉; 王琦媛; 杨同文; 唐跃辉
（周口师范学院生命科学与农学学院，河南周口466001）
Author(s):
XIE Jia-tong; SUN Li-dan; CHEN Xiao-man; FANG Tong-tong; ZHANG Miao-miao; ZHAO Yu-fan; YANG Ran; WANG Qi-yuan; YANG Tong-wen; TANG Yue-hui
（College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466001, China）
关键词:
麻风树; JcWRI1; AP2家族; 转基因水稻; 胚乳
Keywords:
physic nut; JcWRI1; AP2 family; transgenic rice; endosperm
分类号:
S727.32
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.006
文献标志码:
A
摘要:
WRI1是AP2类转录因子的成员，在植物生长发育和脂肪酸合成途径中起着重要的调控作用。通过RT-PCR技术从麻风树中克隆了1个AP2家族基因，将其命名为JcWRI1。JcWRI1基因开放阅读框全长1 137 bp，编码378个氨基酸。表达模式分析结果表明，在麻风树种子胚中没有检测到JcWRI1基因的表达，然而该基因在麻风树种子胚乳中高表达。亚细胞定位结果表明，JcWRI1基因编码1个核定位蛋白质。表型分析结果表明，提高JcWRI1基因的表达量不影响转基因水稻的生长发育，但是改变了转基因植株叶片、胚乳中脂肪酸组分的含量，并且提高了转基因水稻叶片、胚乳中的含油量。qRT-PCR结果表明，脂肪酸合成相关基因在JcWRI1转基因水稻中的相对表达量显著高于野生型。研究结果为将来研究JcWRI1基因在麻风树种子胚乳发育及油脂代谢途径中的功能提供了理论依据和新的基因资源。
Abstract:
WRI1 is a member of AP2 transcription factors and plays an important regulatory role in plant growth and development and fatty acid synthesis. In this study, an AP2 family gene was cloned from physic nut by RT-PCR and named JcWRI1. The open reading frame of JcWRI1 gene was 1 137 bp in length, encoding 378 amino acids. The results of expression pattern analysis showed that the expression of JcWRI1 gene was not detected in the embryo, but it was highly expressed in the endosperm. The subcellular localization results indicated that the JcWRI1 gene encoded a nuclear localization protein. Phenotypic analysis revealed that the increase of JcWRI1 gene expression did not affect the growth and development of transgenic rice, but changed the fatty acid composition in the leaves and endosperm of transgenic plants and increased the oil content in the endosperm and leaves of transgenic rice. The results of qRT-PCR showed that the relative expression of fatty acid synthesis-related genes in JcWRI1 transgenic rice was significantly higher than that in wild-type rice. The results provide a theoretical basis and new genetic resources for further research on the function of JcWRI1 gene in the endosperm development and lipid metabolism pathway of physic nut.

参考文献/References:

[1]FEI W J, YANG S Q, HU J, et al. Research advances of WRINKLED1 (WRI1) in plants[J]. Functional Plant Biology, 2020, 47(3):185-194.
[2]SHOCKEY J, REGMI A, COTTON K, et al. Identification of Arabidopsis GPAT9 (At5g60620) as an essential gene involved in triacylglycerol biosynthesis[J]. Plant Physiology, 2015, 170(1):163-179.
[3]MISRA A, KHAN K, NIRANJAN A, et al. Heterologous expression of two GPATs from Jatropha curcas alters seed oil levels in transgenic Arabidopsis thaliana[J]. Plant Science, 2017, 263(2):79-88.
[4]ZHENG P Z, ALLEN W B, ROESLER K, et al. A phenylalanine in DGAT is a key determinant of oil content and composition in maize[J]. Nature Genetics, 2008, 40(3):367-372.
[5]FOCKS N, BENNING C. WRINKLED1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism[J]. Plant Physiology, 1998, 118(1):91-101.
[6]BAUD S, WUILLME S, TO A, et al. Role of WRINKLED1 in the transcriptional regulation of glycolytic and fatty acid biosynthetic genes in Arabidopsis[J]. Plant Journal, 2009, 60(6):933-947.
[7]SHEN B, ALLEN W B, ZHENG P Z, et al. Expression of ZmLEC1 and ZmWRI1 increases seed oil production in maize[J]. Plant Physiology, 2010, 153(3): 980-987.
[8]GUO W, CHEN L M, CHEN H F, et al. Overexpression of GmWRI1b in soybean stably improves plant architecture and associated yield parameters, and increases total seed oil production under field conditions[J]. Plant Biotechnology Journal, 2020, 18(8): 1639-1641.
[9]LIU J, WEI H, ZHAN G M, et al. Increasing seed mass and oil content in transgenic Arabidopsis by the overexpression of wri1-like gene from Brassica napus[J]. Plant Physiology and Biochemistry, 2010, 48(1):9-15.
[10]EWUNIE G A, MORKEN J, LEKANG O I, et al. Factors affecting the potential of Jatropha curcas for sustainable biodiesel production: a critical review[J]. Renewable and Sustainable Energy Reviews, 2020,137(2):1-18.
[11]AKHTER D, QIN R, NATH U K, et al. A rice gene, OsPL, encoding a MYB family transcription factor confers anthocyanin synthesis, heat stress response and hormonal signaling[J]. Gene, 2019, 699:62-72.
[12]WEI Q, LI J, ZHANG L, et al. Cloning and characterization of a β-ketoacyl-acyl carrier protein synthase Ⅱ from Jatropha curcas[J]. Journal of Plant Physiology, 2012, 169(8):816-824.
[13]LI D L, HE Y J, LI S H, et al. Genome-wide characterization and expression analysis of AP2/ERF genes in eggplant (Solanum melongena L.)[J]. Plant Physiology and Biochemistry, 2021, 167(3):492-503.
[14]MA W, KONG Q, VINCENT A, et al. WRINKLED1, A ubiquitous regulator in oil accumulating tissues from Arabidopsis embryos to oil palm mesocarp[J]. PLoS One, 2013, 8(7):1-13.
[15]王玲，刘晓伟，江纳，等．蔓花生AP2基因家族的生物信息学分析[J]．江苏农业科学，2020，48(14)：65-77．
[16]BAUD S, MENDOZA M S, TO A, et al. WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis[J]. Plant Journal, 2007, 50(5):825-838.
[17]BATES P D, STYMNE S, OHLROGGE J. Biochemical pathways in seed oil synthesis[J]. Current Opinion in Plant Biology, 2013, 16(3):358-364.
[18]BATES P D, FATIHI A, SNAPP A R, et al. Acyl editing and headgroup exchange are the major mechanisms that direct polyunsaturated fatty acid flux into triacylglycerols[J]. Plant Physiology, 2012, 160(3):1530-1539.
[19]BATES P D, BROWSE J. The significance of different diacylgycerol synthesis pathways on plant oil composition and bioengineering[J]. Frontiers in Plant Science, 2012, 3:147.

相似文献/References:

[1]唐跃辉,赵雨凡,蒋心言,等.麻风树JcHDZ28基因克隆与功能分析[J].江苏农业学报,2024,(01):39.[doi:doi:10.3969/j.issn.1000-4440.2024.01.004]
　TANG Yue-hui,ZHAO Yu-fan,JIANG Xin-yan,et al.Cloning and function analysis of JcHDZ28 gene from Jatropha curcas L.[J].,2024,(02):39.[doi:doi:10.3969/j.issn.1000-4440.2024.01.004]

备注/Memo

备注/Memo:
收稿日期：2021-11-05基金项目：2020年度河南省自然科学基金青年项目（202300410520）；2020年度河南省高等学校重点科研项目（21A180028）；2021年度河南省周口师范学院大学生创新创业训练计划项目（S202110478032）；周口师范学院大学生科研创新基金项目（ZKNUD2021073）作者简介：谢佳彤（2000-），女，河南信阳人，本科，主要从事麻风树基因功能研究。（E-mail）2230439874@qq.com通讯作者：唐跃辉，（E-mail）yhtang2005@163.com
更新日期/Last Update: 2022-05-07