[1]时丕彪,王德领,蒋润枝,等.藜麦ZF-HD转录因子的全基因组鉴定及其对盐胁迫的响应分析[J].江苏农业学报,2022,38(02):304-312.[doi:doi:10.3969/j.issn.1000-4440.2022.02.003]
　SHI Pi-biao,WANG De-ling,JIANG Run-zhi,et al.Genome-wide identification of ZF-HD transcription factors and expression analysis of response to salt stress in quinoa[J].,2022,38(02):304-312.[doi:doi:10.3969/j.issn.1000-4440.2022.02.003]
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藜麦ZF-HD转录因子的全基因组鉴定及其对盐胁迫的响应分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
304-312
栏目:
遗传育种·生理生化
出版日期:
2022-04-30

文章信息/Info

Title:
Genome-wide identification of ZF-HD transcription factors and expression analysis of response to salt stress in quinoa
作者:
时丕彪; 王德领; 蒋润枝; 李斌; 晏军; 方迪; 闫凯旋; 顾闽峰
(盐城市新洋农业试验站，江苏盐城224049)
Author(s):
SHI Pi-biao; WANG De-ling; JIANG Run-zhi; LI Bin; YAN Jun; FANG Di; YAN Kai-xuan; GU Min-feng
(Xinyang Agricultural Experiment Station of Yancheng City, Yancheng 224049, China)
关键词:
藜麦; 锌指同源异型结构域（ZF-HD）; 全基因组; 盐胁迫; 基因表达
Keywords:
quinoa; zinc finger-homeodomain (ZF-HD); genome-wide; salt stress; gene expression
分类号:
S512.9
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.003
文献标志码:
A
摘要:
以藜麦的高质量基因组为参考，对藜麦锌指同源异型结构域（ZF-HD）转录因子基因CqZF-HD进行全基因组鉴定，并利用生物信息学方法对其编码的蛋白质理化性质、二级结构、亚细胞定位、保守结构域以及基因结构、系统进化关系进行了分析。同时，利用前期转录组测序结果分析了CqZF-HD家族基因在盐胁迫下的表达模式。结果表明，在藜麦中共鉴定出21个ZF-HD转录因子，大部分定位在细胞质，二级结构以无规则卷曲为主，氨基酸序列长度68～284 aa，相对分子质量7.54×103～30.20×103，理论等电点6.01～9.54；每个ZF-HD蛋白都含有ZF或HD保守结构域，系统进化树将其划分为5个亚家族。盐处理下表达分析结果显示，11个CqZF-HD基因响应藜麦盐胁迫，其中8个基因呈上调表达，3个基因呈下调表达，说明ZF-HD家族基因在参与藜麦盐胁迫中发挥着不同的作用。
Abstract:
Zinc finger-homeodomain (ZF-HD) proteins are plant-specific transcription factors that play important roles in plant growth, development and various stress responses. In this study, the CqZF-HD gene was identified at genome-wide level with the high-quality quinoa genome as a reference. The physicochemical properties, secondary structure, subcellular localization and conserved domains of CqZF-HDs, phylogenetic relationship and gene structure were analyzed by bioinformatics method. Meanwhile, the expression pattern of CqZF-HD family genes under salt stress was also analyzed by preliminary transcriptome sequencing results. The results showed that 21 ZF-HD transcription factors were identified in quinoa, most of which were located in the cytoplasm, the secondary structure was dominated by random coils, amino acid sequence length was 68-284 aa, relative molecular weight was 7.54×103-30.20×103, and theoretical isoelectric point was 6.01-9.54. All of the ZF-HDs were divided into five subfamilies by phylogenetic tree analysis, and each member contained conserved domains ZF or HD. The expression analysis under salt treatment showed that 11 CqZF-HDs responded to salt stress in quinoa, eight genes were up-regulated, and three genes were down-regulated. In conclusion, ZF-HD family genes play different roles in response to salt stress in quinoa.

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备注/Memo

备注/Memo:
收稿日期：2021-07-11基金项目：江苏省农业科技自主创新基金项目[CX(19)3116]；江苏现代农业(蔬菜)产业技术体系(盐城)推广示范基地项目[JATS(2020)205]作者简介：时丕彪(1989-)，男，山东菏泽人，助理研究员，主要从事农作物新品种选育及分子育种研究。(E-mail)1032175660@qq.com通讯作者：顾闽峰，(E-mail)ycgmf@126.com
更新日期/Last Update: 2022-05-07