[1]王娟娟,胡珈玮,狄霖,等.秸秆还田与氮肥运筹对水稻不同生育期土壤细菌群落结构的影响[J].江苏农业学报,2021,(06):1460-1470.[doi:doi:10.3969/j.issn.1000-4440.2021.05.013]
　WANG Juan-juan,HU Jia-wei,DI Lin,et al.Effects of straw returning and nitrogen management on soil microbial community structure at different rice growth stages[J].,2021,(06):1460-1470.[doi:doi:10.3969/j.issn.1000-4440.2021.05.013]
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秸秆还田与氮肥运筹对水稻不同生育期土壤细菌群落结构的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年06期
页码:
1460-1470
栏目:
耕作栽培·资源环境
出版日期:
2021-12-30

文章信息/Info

Title:
Effects of straw returning and nitrogen management on soil microbial community structure at different rice growth stages
作者:
王娟娟1; 胡珈玮1; 狄霖2; 刘玲玲1; 王桂良1; 钱晓晴1; 3; 张振华3; 4
(1.扬州大学环境科学与工程学院，江苏扬州225127;2.镇江市农业农村局，江苏镇江212009；3.扬州大学土壤健康研究所，江苏扬州225127；4.江苏省农业科学院农业资源与环境研究所，江苏南京210014)
Author(s):
WANG Juan-juan1; HU Jia-wei1; DI Lin2; LIU Ling-ling1; WANG Gui-liang1; QIAN Xiao-qing1; 3; ZHANG Zhen-hua3; 4
(1.College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China;2.Zhenjiang Agricultural and Rural Bureau, Zhenjiang 212009, China;3.Institute of Soil Health, Yangzhou University, Yangzhou 225127, China;4.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
秸秆还田; 氮肥; 土壤; 细菌群落结构; 水稻
Keywords:
straw returning; nitrogen fertilization; soil; bacterial community structure; rice
分类号:
S154.3；S511
DOI:
doi:10.3969/j.issn.1000-4440.2021.05.013
文献标志码:
A
摘要:
秸秆还田是当前资源循环利用的重要方式。氮肥运筹对于秸秆还田效率及土壤细菌群落结构的影响较大。本研究采用盆栽试验的方法，探究秸秆还田和不同氮肥运筹条件下水稻主要生育期的土壤细菌群落结构变化。试验设置了常规施氮（T1）、秸秆还田＋氮肥后移（T2）、秸秆还田＋常规施氮（T3）、秸秆还田＋氮肥前移（T4）4个处理，分别测定了水稻苗期、分蘖期、成熟期土壤部分理化性质及细菌群落组成，并于成熟期测产。结果表明，各处理中优势细菌包括Chloroflexi、Proteobacteria、Acidobacteria、Firmicutes、Actinobacteria，总占比＞78%，Chloroflexi在水稻苗期和成熟期占比最高，而Proteobacteria在水稻分蘖期最多。秸秆还田＋氮肥后移处理提高了水稻苗期土壤细菌的丰富度，但对其均匀度影响较小。在水稻分蘖期和成熟期，秸秆还田与否及氮肥运筹对土壤细菌多样性影响较小。秸秆还田提高了水稻产量，以秸秆还田＋氮肥前移处理的增产效果最大，且该处理增加了水稻分蘖期土壤硝态氮、速效磷含量。在水稻成熟期，土壤中与产量呈显著正相关的细菌群落包括Acidobacteria和Gemmatinomadetes等。综上所述，秸秆还田并增加氮肥基施比例可以增加水稻产量，并对改善水稻生长后期土壤细菌群落结构产生积极作用。
Abstract:
Straw returning is an important way of resource recycling. Nitrogen (N) application has great influence on straw returning efficiency and soil bacterial community structure. In this study, a pot experiment was conducted to explore the changes of soil bacterial community structure at different rice growth stages under the treatments of straw returning combined with N management. There were four treatments in the experiment, including conventional N fertilization (T1), straw returning with postponed N fertilization (T2), straw returning with conventional N fertilization (T3) and straw returning with moved forward N fertilization (T4). Soil physiochemical properties and bacterial community composition were determined at seedling, tillering and mature stage, and yield was measured at harvest stage. The results showed that the dominant bacteria phyla in all treatments were Chloroflexi, Proteobacteria, Acidobacteria, Firmicutes and Actinobacteria (together accounted for over 78% of the total sequences). Chloroflexi represented the most dominant phylum at seedling and mature stages, while Proteobacteria was the most abundant phylum at tillering stage. Straw returning with postponed N fertilization increased the richness of soil bacteria, but had little influence on bacterial evenness at seedling stage. Straw returning and nitrogen management had little influence on soil bacterial diversity at tillering and maturity stages of rice. Straw returning increased rice yield, and the highest yield was obtained in the treatment of straw returning with moved forward N fertilization (T4). Moreover, the contents of soil available phosphorus (P) and nitrate nitrogen were increased at tillering stage of rice in T4 treatment. The relative abundance of bacterial communities, including Acidobacteria and Gemmatinomadetes was positively correlated with the yield at ripen stage. In conclusion, straw returning combined with moved forward N fertilization can increase rice yield and improve soil microbial community structure at late growth stage.

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备注/Memo

备注/Memo:
收稿日期：2021-08-24基金项目：国家自然科学青年科学基金项目（41701093）；中国博士后基金项目（2017M611928）；国家重点研发计划课题项目（2017YFD0200107）作者简介：王娟娟(1979-)，女，江苏泗洪人，博士，副教授，主要从事环境微生物学研究。(E-mail)wangjuanjuan@yzu.edu.cn通讯作者：张振华，(E-mail) zhenhuaz70@hotmail.com
更新日期/Last Update: 2022-01-07