[1]王亚丽,陈煜东,王益军.高世代回交玉米矮秆种质的转录组分析[J].江苏农业学报,2021,(02):280-288.[doi:doi:10.3969/j.issn.1000-4440.2021.02.002]
　WANG Ya-li,CHEN Yu-dong,WANG Yi-jun.Transcriptome analysis on the advanced backcross population of maize dwarf germplasm[J].,2021,(02):280-288.[doi:doi:10.3969/j.issn.1000-4440.2021.02.002]
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高世代回交玉米矮秆种质的转录组分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
280-288
栏目:
遗传育种·生理生化
出版日期:
2021-04-30

文章信息/Info

Title:
Transcriptome analysis on the advanced backcross population of maize dwarf germplasm
作者:
王亚丽1; 2; 陈煜东1; 2; 王益军1; 2
（1.江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室/扬州大学农学院，江苏扬州225009;2.江苏省粮食作物现代产业技术协同创新中心/扬州大学，江苏扬州225009）
Author(s):
WANG Ya-li1; 2; CHEN Yu-dong1; 2; WANG Yi-jun1; 2
（1.Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/College of Agriculture, Yangzhou University, Yangzhou 225009, China;2.Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Yangzhou University, Yangzhou 225009, China）
关键词:
矮秆; 高世代回交群体; 转录组测序; 共表达; 玉米
Keywords:
dwarf plant; advanced backcross population; transcriptome sequencing; co-expression; maize
分类号:
S513.035.3
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.002
文献标志码:
A
摘要:
株高影响株型建成，与抗倒性、收获指数以及最终产量相关。对矮秆材料的研究有助于丰富对株高调控机理的认识。在前期研究中，对玉米矮秆材料D11的农艺与生理特征进行了分析。本研究基于高世代回交群体，通过转录组测序，对矮秆材料D11的基因表达调控进行解析。与株高正常植株相比，矮秆材料中共检测到2 537个差异表达的基因，其中1 120个基因表达上调、1 417个基因表达下调。功能注释、GO富集、通路富集的结果表明，差异表达基因参与细胞延伸、细胞骨架功能、微管组织、叶绿体功能、植物激素合成代谢等。差异表达基因最显著富集的通路与糖酵解相关。共表达分析结果表明，编码果糖-1,6-双磷酸酶的基因与多个差异表达基因存在功能关联。该研究结果揭示了玉米矮秆材料中的基因表达调控，为后续玉米株高调控节点的发掘提供了参考。
Abstract:
Plant height affects plant type and is related to lodging resistance, harvest index and final yield. The research on dwarf plants helps to improve the understanding of plant height regulation mechanism. In previous studies, the agronomic and physiological characteristics of maize dwarf material D11 were analyzed. In this study, based on the advanced backcross population, the gene expression regulation of D11 was analyzed by transcriptome sequencing. A total of 2 537 differentially expressed genes (DEGs) were identified,including 1 120 up-regulated and 1 417 down-regulated DEGs. Functional annotation, GO enrichment and pathway enrichment analysis results indicated that DEGs were involved in cell expansion, cytoskeletal function, microtubule organization, chloroplast function, phytohormone homeostasis. The pathway most significantly enriched by DEGs was related to glycolysis. The gene encoding fructose-1,6-bisphosphatase was functionally related to several DEGs. The results of this study reveal the gene expression regulation in maize dwarf materials, and provide a reference for the subsequent exploration of maize plant height regulatory modes.

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备注/Memo

备注/Memo:
收稿日期：2020-07-06基金项目：国家自然科学基金项目(31571671)；扬州大学高端人才支持计划项目(18HTYZU12)；扬州大学青蓝工程项目(QLYZU201809)；扬州大学科技创新培育基金项目(2019CXJ097)；江苏高校优势学科建设工程项目(PAPD)作者简介：王亚丽(1995-),女,河南济源人,硕士研究生，研究方向为玉米遗传育种。(E-mail)wylyzu@163.com通讯作者：王益军,(E-mail)wyj@yzu.edu.cn
更新日期/Last Update: 2021-05-10