[1]范亚磊,赵敏,邓晟,等.侵染江苏猕猴桃的北方根结线虫（Meloidogyne hapla）形态学描述和分子特征分析[J].江苏农业学报,2021,(01):75-82.[doi:doi:10.3969/j.issn.1000-4440.2021.01.010]
　FAN Ya-lei,ZHAO Min,DENG Sheng,et al.Morphological description and molecular characteristic analysis on Meloidogyne hapla which infect kiwifruit in Jiangsu province[J].,2021,(01):75-82.[doi:doi:10.3969/j.issn.1000-4440.2021.01.010]
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侵染江苏猕猴桃的北方根结线虫（Meloidogyne hapla）形态学描述和分子特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
75-82
栏目:
植物保护
出版日期:
2021-02-28

文章信息/Info

Title:
Morphological description and molecular characteristic analysis on Meloidogyne hapla which infect kiwifruit in Jiangsu province
作者:
范亚磊1; 2; 赵敏1; 邓晟1; 刘瑞显3; 姚焕钊4; 魏利辉1; 冯辉1
（1.江苏省农业科学院植物保护研究所，江苏南京210014；2.扬州大学园艺与植物保护学院，江苏扬州225009；3.江苏省农业科学院经济作物研究所，江苏南京210014；4.徐州市铜山区植物保护站，江苏徐州221116）
Author(s):
FAN Ya-lei1; 2; ZHAO Min1; DENG Sheng1; LIU Rui-xian3;  YAO Huan-zhao4; WEI Li-hui1; FENG Hui1
（1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014，China；2.School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009，China；3.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014，China；4.Plant Protection Station of Tongshan District of Xuzhou City, Xuzhou 221116，China）
关键词:
猕猴桃; 根结线虫; 北方根结线虫; 内转录间隔区（ITS）; COⅡ; 进化分析
Keywords:
kiwifruit; root-knot nematode; Meloidogyne hapla; internal transcribed spacer(ITS); COⅡ; phylogenetic analysis
分类号:
S436.634
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.010
文献标志码:
A
摘要:
根结线虫病长期以来对猕猴桃安全生产造成了严重威胁。调查发现江苏省连云港市部分猕猴桃种植园根结线虫病发生普遍，组织切片可见根部出现根结线虫诱导的特异的巨细胞。形态学观察和分子检测结果显示，尽管与其他群体存在一定差异，但仍确定本次分离自猕猴桃的根结线虫（种群编号：CN43）为北方根结线虫（Meloidogyne hapla）。基于内转录间隔区（ITS）和COⅡ-16S序列构建的遗传进化树显示，CN43分离群体与已知的北方根结线虫位于同一进化分支，而与其他种类的根结线虫明显区分开来。以ITS序列构建的中接网络将34个北方根结线虫分离群体划分为10个单倍型，其中CN43属于最大的单倍型群组；然而，单倍型分析不能确定各分离群体在地理分布上的关联性。综上，本研究首次报道了北方根结线虫在中国猕猴桃上的侵染和发生，其分离群体的形态学描述和分子特征分析可为揭示猕猴桃根结线虫的种群多样性和种间变异提供参考。
Abstract:
Root-knot diseases caused by Meloidogyne spp. have posed great threat to global kiwifruit industry for a long time. It was found through investigation that, the disease caused by root-knot nematode was common in part of the kiwifruit plantations in Lianyungang City, Jiangsu province. The specific giant cells were found in tissue slices of roots, which may be induced by root-knot nematodes. Results of morphological observation and molecular detection indicated that, the root-knot nematode (population code: CN43) separated from kiwifruits was confirmed to be Meloidogyne hapla, although there was difference compared with other polulations. Phylogenetic trees inferred from internal transcribed spacer (ITS) and partial COⅡ-16S sequences revealed that, isolate population of CN43 and M. hapla were within the same clade and could be separated from other root-knot nematode species obviously. Isolates of 34 M. hapla in NCBI database were divided into ten haplotypes using median-joining network constructed by ITS sequence, in which CN43 belonged to the largest haplotype group. However, haplotype analysis could hardly show the correlation of geographic distribution between the isolates. In conclusion, infection and occurrence of M. hapla in kiwifruit trees of China was reported for the first time. Analysis on the morphology and molecular characteristics of the isolated M. hapla populations can provide reference for revealing population diversity and intraspecific variability of root-knot nematodes in kiwifruit.

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备注/Memo

备注/Memo:
收稿日期：2020-06-20基金项目：国家自然科学基金项目（31871943）; 江苏省农业科技自主创新基金项目[CX(18)2005]; 江苏省农业科学院“小而特”学科建设项目[ZX(19)6005]作者简介：范亚磊(1994-)，男，江苏淮安人，硕士研究生，从事植物线虫病害防治研究。通讯作者：冯辉,(Tel)025-84390769;(E-mail)fenghui@jaas.ac.cn
更新日期/Last Update: 2021-03-15