[1]张升,张伟,王丽,等.氨基酸态氮对禾谷镰刀菌生长及DON毒素产生的影响[J].江苏农业学报,2020,(06):1403-1410.[doi:doi:10.3969/j.issn.1000-4440.2020.06.008]
　ZHANG Sheng,ZHANG Wei,WANG Li,et al.Effects of amino acid nitrogen on the growth of Fusarium graminearum and DON toxin production[J].,2020,(06):1403-1410.[doi:doi:10.3969/j.issn.1000-4440.2020.06.008]
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氨基酸态氮对禾谷镰刀菌生长及DON毒素产生的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1403-1410
栏目:
植物保护
出版日期:
2020-12-31

文章信息/Info

Title:
Effects of amino acid nitrogen on the growth of Fusarium graminearum and DON toxin production
作者:
张升1; 张伟2; 王丽3; 李孝军4; 崔俊涛1
(1.吉林农业大学资源与环境学院，吉林长春130118；2.长春职业技术学院，吉林长春130033；3.长春市朝阳区农业技术推广站，吉林长春130012；4.长春市土壤肥料工作站，吉林长春130033)
Author(s):
ZHANG Sheng1; ZHANG Wei2; WANG Li3; LI Xiao-jun4; CUI Jun-tao1
(1.School of Resources and Environment, Jilin Agricultural University, Changchun 130118, China;2.Changchun Vocational and Technical College, Changchun 130033, China;3.Agricultural Technology Extension Station of Chaoyang District, Changchun City, Changchun 130012, China;4.Changchun Soil and Fertilizer Workstation, Changchun 130033, China)
关键词:
禾谷镰刀菌; 氨基酸态氮; 赖氨酸; DON毒素
Keywords:
Fusarium graminearum; amino acid nitrogen; lysine; DON toxin
分类号:
S154.34
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.008
文献标志码:
A
摘要:
为阐明土壤不同氮素形态尤其是有机氮形态与土壤有害生物种群发生、发展的关系，以禾谷镰刀菌供试菌株，初步研究了5种氨基酸态氮对其生长及DON毒素产生的影响。结果表明：0.20%氮源添加量下的100 ml液体培养基中，各培养基中菌丝干质量差异显著，培养基中菌丝干质量从大到小的顺序为赖氨酸培养基(1.27 g)＞硝酸钠培养基(0.88 g)＞谷氨酸培养基(0.84 g)＞酪氨酸培养基(0.52 g)＞缬氨酸培养基(0.45 g)＞蛋氨酸培养基(0.36 g)。赖氨酸培养基发酵液中DON毒素含量最高，达到2 951 μg/L,其他不同氮源培养基发酵液中DON毒素含量大小顺序为酪氨酸(1 418 μg/L)＞谷氨酸(240 μg/L)＞硝酸钠(224 μg/L)＞缬氨酸(214 μg/L)＞蛋氨酸(50 μg/L)，毒素含量与氮源添加量呈正相关关系。生物测定结果显示，赖氨酸液体培养基发酵液的抑制效果最为明显，种子萌发抑制率、胚根抑制率、胚芽抑制率分别达到了63.0%、60.9%、63.0%，其次为酪氨酸(41.0%、34.4%、34.8%)、谷氨酸(26.0%、18.8%、23.9%)、硝酸钠(25.0%、15.6%、21.7%)、缬氨酸(25.0%、14.1%、21.7%)和蛋氨酸(7.0%、9.4%、17.4%)。对比5种氨基酸，当赖氨酸作为唯一氮源时，对禾谷镰刀菌的生长和产毒作用显著，可提高病原菌的侵染、定殖寄主植物的能力，从而诱导茎腐病的发生。
Abstract:
To elucidate the relationship between different nitrogen forms, especially organic nitrogen forms, and the occurrence and development of soil pest, the effects of five amino acid nitrogen on the growth of Fusarium graminearum and the production of DON toxin were studied by using Fusarium graminearum strains. The results showed that the mycelial dry weight in each medium was significantly different when 100 ml liquid medium was added with 0.20% nitrogen source, and the mycelial dry weight in mediums followed the order of lysine medium (1.27 g)> sodium nitrate medium (0.88 g)> glutamic acid medium (0.84 g)> tyrosine medium(0.52 g)> valine medium(0.45 g)> methionine medium(0.36 g). The content of DON toxin was the highest in the fermentation broth of lysine medium, which reached 2 951 μg/L. The content of DON toxin in the fermentation broth of different nitrogen sources followed the order of tyrosine medium(1 418 μg/L)> glutamic acid medium(240 μg/L)> sodium nitrate medium (224 μg/L)> valine medium(214 μg/L)> methionine medium(50 μg/L). Toxin content was positively correlated with nitrogen source addition. The results of bioassay showed that lysine liquid medium had the most obvious inhibitory effect on seed germination, radicle and germ, inhibition rates were 63.0%, 60.9% and 63.0% , respectively, followed by tyrosine medium(41.0%, 34.4%, 34.8%), glutamate medium (26.0%, 18.8%, 23.9%) , sodium nitrate medium (25.0%, 15.6%, 21.7%), valine medium (25.0%, 14.1%, 21.7%), methionine medium(7.0%, 9.4%, 17.4%). When lysine was used as the only nitrogen source, it had significant effect on the growth and toxin production of Fusarium Graminearum, which could increase the ability of pathogen to infect and colonize the host plant, and induce stem rot.

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备注/Memo

备注/Memo:
收稿日期：2020-03-10基金项目：国家重点研发计划项目(2017YFD0200600)作者简介：张升(1992-)，男，吉林白山人，硕士研究生，研究方向为环境污染生物防控技术与工程。(E-mail)18953479609@163.com通讯作者：崔俊涛，(E-mail)1597060453@qq.com
更新日期/Last Update: 2021-01-15