[1]陈柯源,宋向东,程峰,等.当归补血汤发酵产物对肉鸡免疫和抗氧化能力的影响[J].江苏农业学报,2020,(04):992-999.[doi:doi:10.3969/j.issn.1000-4440.2020.04.026]
　CHEN Ke-yuan,SONG Xiang-dong,CHENG Feng,et al.Effects of fermentation products of Danggui Buxue Tang on immune and antioxidant capacity in broilers[J].,2020,(04):992-999.[doi:doi:10.3969/j.issn.1000-4440.2020.04.026]
点击复制

当归补血汤发酵产物对肉鸡免疫和抗氧化能力的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年04期
页码:
992-999
栏目:
畜牧兽医·水产养殖
出版日期:
2020-08-31

文章信息/Info

Title:
Effects of fermentation products of Danggui Buxue Tang on immune and antioxidant capacity in broilers
作者:
陈柯源1; 宋向东1; 程峰1; 张玉珺2; 梁剑平1; 郝宝成1
(1.中国农业科学院兰州畜牧与兽药研究所/农业农村部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室, 甘肃兰州730050；2.甘肃农业大学，甘肃兰州730050)
Author(s):
CHEN Ke-yuan1; SONG Xiang-dong1; CHENG Feng1; ZHANG Yu-jun2; LIANG Jian-ping1; HAO Bao-cheng1
(1.Key Lab of New Animal Drug Project Gansu Province/Key Laboratory of Veterinary Pharmaseutical Development, Ministry of Agriculture and Rural Affairs/Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agriculture Sciences, Lanzhou 730050, China；2.Gansu Agricultural University, Lanzhou 730050, China)
关键词:
当归补血汤; 发酵; 生产性能; 免疫功能; 抗氧化能力; 十二指肠绒毛形态
Keywords:
Danggui Buxue Tang(DBT）; fermentation; growth performance; immune function; antioxidant capacity; duodenal villus morphology
分类号:
S831.5
DOI:
doi:10.3969/j.issn.1000-4440.2020.04.026
文献标志码:
A
摘要:
本研究拟通过明确当归补血汤(Danggui buxue tang，DBT）发酵产物对肉鸡生长性能、免疫功能、抗氧化能力以及十二指肠绒毛形态的影响，为新型中药发酵饲料添加剂的研发提供理论依据。试验选用1日龄健康白羽肉鸡210只，随机分成5组：饲喂基础日粮的对照组、在日粮中添加1.0% DBT药粉的未发酵组以及在日粮中分别添加0.5%、1.0%和2.0% DBT发酵物的发酵组。结果显示，与对照组和DBT未发酵组相比，1.0%和2.0% DBT发酵组在生产性能方面，可显著降低肉鸡日采食量(P＜0.05）和料肉比(P＜0.05），提高肉鸡平均日增质量(P＜0.05）；在免疫性能方面，试验21d时，法氏囊指数、新城疫抗体效价以及白细胞介素2 (IL-2)、白细胞介素6 (IL-6)、肿瘤坏死因子α(TNF-α）和干扰素(INF-γ）含量均显著升高(P＜0.05）；在抗氧化性能方面，血清超氧化物歧化酶(SOD）、谷胱甘肽过氧化物酶(GSH-Px）以及总抗氧化能力(T-AOC）显著升高 (P＜0.05），血清中丙二醛(MDA）含量显著降低(P＜0.05）。在十二指肠绒毛形态方面，试验42 d时，1.0% DBT发酵组的绒毛高度/隐窝深度比值同样显著高于对照组和DBT未发酵组(P＜0.05）。研究结果表明，日粮中添加1.0%的DBT发酵产物能够对白羽肉鸡的生长、免疫、抗氧化性能及十二指肠绒毛形态有显著的改善作用，且饲喂效果明显优于DBT未发酵组和空白对照组，DBT发酵产物可作为一种新型饲料添加剂应用于畜禽养殖业。
Abstract:
This study intends to provide theoretical basis for the research and development of new Chinese medicine fermented feed additives by clarifying the effects of fermentation products of Danggui Buxue Tang (DBT) on the growth performance, immune function, antioxidant capacity and duodenal villi morphology of broilers. In this study, a total of 210 healthy white feather broilers with similar initial body weight(one day old) were randomly divided into five groups. The control group was fed with the basic diet, the unfermented group was fed with basic diet and 1.0% DBT powder, and the fermentation group was fed with basic diet and 0.5%, 1.0% and 2.0% DBT fermentation products, respectively. The results showed that compared with the control and unfermented groups, 1.0% DBT and 2.0% DBT fermentation groups significantly reduced the daily feed intake (P＜0.05） and feed to gain ratio(F/G) (P＜0.05）, and increased the average daily gain (P＜0.05） in growth performance. Moreover, the index of bursa of Fabricius, ND-HI immune globulin and contents of interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor α(TNF-α） and interferon-γ(INF-γ） were significantly increased in immune function (P＜0.05）. In addition, the contents of superoxide dismutase(SOD） and glutathione peroxidase(GSH-Px）and total antioxidant capacity (T-AOC） in antioxidant capacity were significantly improved (P<0.05), but the content of malondialdehyde (MDA） in serum was significantly reduced (P＜0.05）. In terms of duodenal villi morphology， the ratio of villus height to crypt depth of 42-day-old broilers in 1.0% DBT fermentation group was also significantly higher than that in control group and unfermented group (P<0.05). The results showed that the addition of 1.0% fermentation products of DBT could significantly promote the growth, immune and antioxidant capacity of white feather broilers, and had a significant effect on the duodenal villus morphology. The feeding effect of 1.0% DBT fermentation group is obviously better than that of unfermented group and control group, and fermentation products of DBT can be used in livestock and poultry breeding as a new type of feed additive.

参考文献/References:

[1]LI X T, WANG B, LI J L,et al.Effects of Dangguibuxue tang,a Chinese herbal medicine,on growth performance and immune responses in broiler chicks[J].Biological Research,2013，46(2):183-188.
[2]谢东杰,王爱迪,刘宝山.当归补血汤在血液疾病中作用机制的研究进展[J].中华中药杂志,2018,33(6):2488-2490.
[3]李爽,王海君,高博,等.当归补血汤清除DPPH自由基能力的测定[J].齐齐哈尔医学院学报,2018,39(8):916-917.
[4]曾宇,张三印,胡冠英.当归补血汤的研究进展[J].时珍国医国药,2016,27(2):422-424.
[5]GONG A G W, LAU K M, ZHANG L M L,et al.Danggui buxue tang,Chinese herbal decoction containing astragali raix and angelicae sinensis radix,induces production of nitric oxide in endothelial cells:signaling mediated by phosphorylation of endothelial nitric oxide synthase[J].Planta Medica,2016,82(5):418-423.
[6]AMY GUO-WEI G, NING L, KEI-MAN L,et al.Calycosin orchestrates the functions of Danggui buxue tang,a Chinese herbal decoction composing of astragali radix and angelica sinensis radix: an evaluation by using calycosin knock out herbal extract[J]. Journal of Ethnopharmacology,2015,168:150-157.
[7]ZHANG Y W, XIE D, XIA B,et al.Suppression of transforming growth factor-beta1 gene expression by Danggui buxue tang,a traditional Chinese herbal preparation,in retarding the progress of renal damage in streptozotocin-induced diabetic rats[J].Hormone and Metabolic Research,2006,38(2):82-88.
[8]ZHENG K Y Z, CHOI R C Y, CHEUNG A W H,et al.Flavonoids from radix astragali induce the expression of erythropoietin in cultured cells: a signaling mediated via the accumulation of hypoxia-inducible factor-1α[J]. Journal of Agricultural & Food Chemistry,2011,59(5):1697-1704.
[9]SONNENBURG J L, BCKHED F. Diet-microbiota interactions as moderators of human metabolism[J].Nature,2016,535(7610):56-64.
[10]李宗杰.复方发酵中药对肉鸡生产性能的影响[J].畜牧兽医科技信息,2019(4):36.
[11]韩春杨,刘翠艳,牛钟相.中药制剂发酵前后成分的变化及对肉鸡部分免疫指标和生长的影响[J].畜牧兽医学报,2005,36(11):1223-1227.
[12]董永军,王丽荣,王宪文,等.甘草多糖的提取及其对肉仔鸡免疫功能的影响[J].江苏农业学报,2008,24(5):158-162.
[13]张晓静,史洪涛,牛湘楠,等.发酵黄芪-甘草水提物对817肉鸡生长性能、免疫器官指数和肉品质的影响[J].中国畜牧兽医,2018, 5(4): 933-939.
[14]乔宏兴,史洪涛,宋予震,等.发酵黄芪对肉鸡生长性能、抗氧化功能的影响[J].中国畜牧兽医,2018,45(3):705-711.
[15]HMANI H, DAOUD L, JLIDI M,et al.A Bacillus subtilis strain as probiotic in poultry:selection based on in vitro functionalproperties and enzymatic potentialities[J].Journal of Industrial Microbiology & Biotechnology,2017,44(8):1157-1166.
[16]RHAYAT L, JACQUIER V, BRINCH K S,et al.Bacillus subtilis strain specificity affects performance improvement in broilers [J]. Poultry Science,2017,96(7): 2274-2280.
[17]刘晓婷,朱晓庆,杨莉,等.中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡免疫活性的影响[J].西北农业学报,2018,27(9):35-42.
[18]孙跃博,罗瑞卿,陈洁,等.中药对围产期奶牛血液免疫及抗氧化活性指标的影响[J].西北农业学报,2015,24(11):22-27.
[19]MAROUFYAN E.Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers[J].Lipids in Health & Disease,2012,11(1):15.
[20]ZHANG Q，LIN Z.Effect of Ganoderma lucidum polysaccharides B on TNFalpha and IFNgamma production and their mRNA expression[J].Journal of Beijing Medical University,1999,31(2): 179-183.
[21]刘燕,袁律峰,季权安,等.冷应激对兔波氏杆菌感染传播的影响[J].农业生物技术学报,2019,27(2):121-128.
[22]乔宏兴,史洪涛,王永芬,等.黄芪-枯草芽孢杆菌复合发酵制剂对肉鸡生产性能及免疫功能的影响[J].中国兽医学报,2015,35(7):1181-1186.
[23]谢全喜,张建梅,于佳民,等.鼠李糖乳杆菌发酵中草药复合枯草芽孢杆菌对黄羽肉鸡生长后期免疫性能及大肠杆菌感染的研究[J].中国畜牧兽医,2016,43(6):1523-1529.
[24]李晴晴,王承民,静罗,等.饲粮中添加浒苔多糖对肉鸡生长性能和免疫功能的影响[J].中国家禽,2017,39(9):24-28.
[25]LOCHMILLER R L,DEERENBERG C.Trade-offs in evolutionary immunology:just what is the cost of immunity?[J].Oikos,2000,88(1):87-98.
[26]杨兵,李晓凤,夏先林.牛膝多糖对断奶仔猪氧化应激和免疫功能的影响[J].江苏农业学报,2017，33(3):618-623.
[27]萨仁娜,佟建明,何春年,等.海带岩藻聚糖及其级分在氧化应激条件下对肉鸡巨噬细胞免疫功能的影响[J].中国农业科学,2008,41(10):3256-3263.
[28]马绍伟,邱其华,叶文林,等.“芪苓散”超微粉对河田鸡血清抗氧化性能及免疫生化指标的影响[J].中国家禽,2016,38(10):21-26.
[29]安胜英,马学会,刘观忠.发酵中药添加剂对肉仔鸡免疫机能和抗氧化机能的影响[J].畜牧兽医学报,2015,46(5):863-867.
[30]张桂枝,靳双星,王学强,等.补中益气汤发酵制剂对肉鸡血清生化指标及抗氧化性能的影响[J].中国家禽,2019,41(7):55-57.
[31]GHAYOUR-NAJAFABADI P,KHOSRAVINIA H,GHEISARI A,et al.Productive performance,nutrient digestibility and intestinal morphometry in broiler chickens fed corn or wheat-based diets supplemented with bacterial- or fungal-originated xylanase[J].Italian Journal of Animal Science,2017, 17(1):1-10.
[32]黄婧溪,李忠良,臧旭鹏,等.不同来源木聚糖酶及其组合对肉鸡肠道黏膜形态与二糖酶活性及其基因表达的影响[J].动物营养学报,2018,30(6):2271-2280.
[33]苏家宜,孔祥峰,李华伟,等.芪楂口服液药渣对断奶仔猪生长性能和肠道健康的影响[J].动物营养学报,2017,29(5):1730-1738.
[34]徐丽娜,郭小军,刘若楠,等.枯草芽孢杆菌E-8对大肠杆菌感染肉鸡生产性能和免疫功能的影响[J].中国家禽,2017,39(24):18-22.

相似文献/References:

[1]董静,王利国.甘蓝发酵过程中亚硝酸盐含量的变化规律及降低方法[J].江苏农业学报,2015,(05):1161.[doi:doi:10.3969/j.issn.1000-4440.2015.05.034]
　DONG Jing,WANG Li-guo.Variation of nitrite content during cabbage old brine fermentation and method of reduction[J].,2015,(04):1161.[doi:doi:10.3969/j.issn.1000-4440.2015.05.034]
[2]朱莉莉,李林强,张宝善,等.橡子粉发酵柠檬酸[J].江苏农业学报,2019,(02):445.[doi:doi:10.3969/j.issn.1000-4440.2019.02.027]
　ZHU Li-li,LI Lin-qiang,ZHANG Bao-shan,et al.Citric acid fermentation of acorn powder[J].,2019,(04):445.[doi:doi:10.3969/j.issn.1000-4440.2019.02.027]

备注/Memo

备注/Memo:
收稿日期：2020-01-07基金项目：甘肃省现代农业产业技术体系项目；中国农业科学院重大产出科研项目(CAAS-ZDXT2018008）作者简介：陈柯源(1993-）, 男,河南洛阳人，硕士研究生, 研究方向为基础兽医学。(E-mail）c18336733159@163.com通讯作者：郝宝成，(E-mail）haobaocheng@sina.cn
更新日期/Last Update: 2020-09-08