[1]马海建,王利娟,江晨舟,等.固相萃取-高效液相色谱法测定保健食品中8种皂苷化合物含量[J].江苏农业学报,2020,(03):743-750.[doi:doi:10.3969/j.issn.1000-4440.2020.03.029]
　MA Hai-jian,WANG Li-juan,JIANG Chen-zhou,et al.Determination of eight saponins contents in health-care food by solid phase extraction-high performance liquid chromatography[J].,2020,(03):743-750.[doi:doi:10.3969/j.issn.1000-4440.2020.03.029]
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固相萃取-高效液相色谱法测定保健食品中8种皂苷化合物含量()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
743-750
栏目:
加工贮藏·质量安全
出版日期:
2020-06-30

文章信息/Info

Title:
Determination of eight saponins contents in health-care food by solid phase extraction-high performance liquid chromatography
作者:
马海建; 王利娟; 江晨舟; 邵明华; 张驰中
（上海安谱实验科技股份有限公司，上海201600）
Author(s):
MA Hai-jian; WANG Li-juan; JIANG Chen-zhou; SHAO Ming-hua; ZHANG Chi-zhong
(ANPEL Laboratory Technologies Inc., Shanghai 201600, China)
关键词:
保健食品; 皂苷化合物; 固相萃取; 高效液相色谱法（HPLC）
Keywords:
health-care food; saponins; solid phase extraction; high performance liquid chromatography(HPLC)
分类号:
TS207.3
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.029
文献标志码:
A
摘要:
旨在建立一种固相萃取结合高效液相色谱检测保健食品中8种皂苷化合物（三七皂苷R1与人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rd）的方法，考察不同溶剂和不同超声时间对皂苷化合物提取效率的影响以及不同固相萃取小柱对回收率的影响。通过试验，确定先用30%甲醇溶液超声20 min进行提取，然后用C18小柱净化的前处理方法，并对优化后的方法进行方法学验证。结果表明，优化后的前处理方法+高效液相色谱法操作简便、耗时少，8种皂苷化合物峰面积与含量线性方程的决定系数均可达到0.999 0以上，检出限为3.7～11.4 μg/g，方法的精密度和重复性良好，样品在24 h内稳定，样品的加标回收率为85.38%～108.41%。该方法可操作性强、稳定性好，可以同时检测含有三七、人参、西洋参和高丽参等原料的保健食品中8种皂苷化合物含量。
Abstract:
The research aims to detect eight saponins including notoginsenoside R1, ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2 and Rd in health-care food by the method of solid phase extraction-high performance liquid chromatography (SPE-HPLC). Effects of different solvents and ultrasonic time on the extraction efficiency of saponins and the influence of different solid phase extraction columns on the recovery rate were investigated. The pretreatment method was determined that ultrasonic extraction was performed by using 30% methanol solution for 20 minutes, and then the C18 solid phase extraction column was used for purification. The methodology validation was applied to evaluate the optimized methods. The results showed that the optimized pretreatment method combined with HPLC was easy to operate and took less time. The determination coefficients of peak area and content of eight saponins were all above 0.999 0 and the detection limit was 3.7-11.4 μg/g. The method showed good precision and reproducibility, and the sample was stable within 24 hours. The recovery rate of standard addition was 85.38%-108.41%. The method was stable and easy to operate, and could be used to detect the contents of eight saponins in health-care food using Panax notoginseng, Panax ginseng, Panax quinquefolius as raw materials.

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备注/Memo

备注/Memo:
收稿日期：2019-11-30作者简介：马海建（1990-），男，河北邢台人，硕士，研究方向为分析化学。（E-mail）hjma0504@163.com通讯作者：张弛中，（E-mail）zhangchizhong@anpel.com.cn
更新日期/Last Update: 2020-07-14