[1]徐文杰,韩士群,周庆,等.饲料中添加抗氧化剂对鲫鱼抵抗藻毒素作用的影响[J].江苏农业学报,2020,(02):417-422.[doi:doi:10.3969/j.issn.1000-4440.2020.02.023]
　XU Wen-jie,HAN Shi-qun,ZHOU Qing,et al.Effect of dietary antioxidant on microcystin-induced toxicosis in crucian[J].,2020,(02):417-422.[doi:doi:10.3969/j.issn.1000-4440.2020.02.023]
点击复制

饲料中添加抗氧化剂对鲫鱼抵抗藻毒素作用的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
417-422
栏目:
畜牧兽医·水产养殖
出版日期:
2020-04-30

文章信息/Info

Title:
Effect of dietary antioxidant on microcystin-induced toxicosis in crucian
作者:
徐文杰; 韩士群; 周庆; 巫佳杰; 陈婷
（江苏省农业科学院农业资源与环境研究所，农业部长江下游平原农业环境重点实验室，江苏南京210014）
Author(s):
XU Wen-jie; HAN Shi-qun; ZHOU Qing; WU Jia-jie; CHEN Ting
(Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
α-硫辛酸; 抗坏血酸; 微囊藻毒素; 鲫鱼; 抗氧化能力
Keywords:
α-lipoic acid; ascorbic acid; microcystin; crucian; antioxidant capacity
分类号:
S963.73
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.023
文献标志码:
A
摘要:
通过40 d的体表暴露试验，研究了饲料中添加α-硫辛酸、抗坏血酸（VC）对鲫鱼暴露于铜绿微囊藻污染水体的保护作用。试验期间水体中微囊藻毒素-LR（MC-LR）质量浓度维持在19.40 μg/L左右，试验组饲料中α-硫辛酸和VC的添加量均为600 mg/kg，测定鲫鱼生长性能、MC-LR解毒效果和肝脏抗氧化能力。结果表明，与负对照组相比，饲料中添加α-硫辛酸组和VC组鲫鱼增质量率显著提高（P<0.05），分别提高了4.91%和3.21%；鲫鱼肌肉中MC-LR含量显著降低（P<0.05），分别降低了40.72%、14.20%；肝脏谷胱甘肽过氧化物酶（GSH-Px）活性、超氧化物歧化酶（SOD）活性、总抗氧化能力（T-AOC）显著提高（P<0.05），分别提高了79.35%和72.53% 、79.35%和72.53%、34.93%和24.43%；肝脏丙二醛（MDA）含量显著降低（P<0.05），分别降低了57.26%和48.86%。可见，饲料中添加α-硫辛酸、VC能够显著提高鱼体的抗氧化能力，减轻MC-LR对鱼体的氧化损伤，进而减少MC-LR在鱼体内的积累，减轻MC-LR对鲫鱼的毒害作用。
Abstract:
In this study, the effects of dietary α-lipoic acid and ascorbic acid (VC) on microcystin-induced toxicosis in crucian were investigated by 40-day’s exposure test. During the test, the mass concentration of microcystin-LR (MC-LR) in water was maintained at about 19.40 μg/L. The additions of α-lipoic acid and VC in the experimental group were 600 mg/kg, and the growth performance, MC-LR detoxification and liver antioxidant capacity of the crucian were investigated. Compared with the negative control group, the weight gain rate increased by 4.91% and 3.21% in crucian fed with α-lipoic acid and ascorbic acid （P<0.05）, meanwhile, the MC-LR content in the muscle decreased by 40.72% and 14.20% （P<0.05）, respectively. Furthermore, activity of glutathione peroxidase (GSH-Px) increased by 79.35% and 72.53% （P<0.05）, the activity of superoxide dismutase (SOD) increased by 79.35% and 72.53% （P<0.05）, and the total antioxidant capacity (T-AOC) increased by 34.93% and 24.43% （P<0.05）, malondialdehyde (MDA) content decreased by 57.26% and 48.86% （P<0.05） in crucian fed with α-lipoic acid and VC. In conclusion, dietary α-lipoic acid and VC can effectively improve the antioxidant capacity of crucian, reduce the oxidative damage and the accumulation of MC-LR.

参考文献/References:

[1]孙志伟，邱丽华，曹煜成，等. 蓝藻水华对水产养殖业影响的研究进展[J]. 生态科学, 2017(1): 231-235.
[2]田娟，余博识，JAMES S D，等. 养殖水体中藻毒素污染问题的研究进展[J]. 水生态学杂志, 2009(5): 99-102.
[3]张新农，刘爱民，桑明远，等. 利用小球藻防控养殖水体蓝藻水华的初步研究[J]. 当代水产, 2014(3): 82-83.
[4]CHEN L, CHEN J, ZHANG X, et al. A review of reproductive toxicity of microcystins[J]. Journal of Hazardous Materials, 2016, 301: 381-399.
[5]CHEN J, XIE P, ZHANG D, et al. In situ studies on the distribution patterns and dynamics of microcystins in a biomanipulation fish-bighead carp (Aristichthys nobilis)[J]. Environmental Pollution, 2007, 147(1): 150-157.
[6]贾军梅，罗维，吕永龙. 微囊藻毒素在太湖白鲢体内的累积规律及其影响因素[J]. 生态毒理学报, 2014, 9(2): 382-390.
[7]MOHAMED Z A, CARMICHAEL W W, HUSSEIN A A. Estimation of microcystins in the freshwater fish Oreochromis niloticus in an Egyptian fish farm containing a Microcystis bloom[J]. Environmental Toxicology, 2003, 18(2): 137-141.
[8]ZHANG D, XIE P, CHEN J, et al. Determination of microcystin-LR and its metabolites in snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) from Lake Taihu, China[J]. Chemosphere, 2009, 76(7): 974-981.
[9]GAN N, MI L, SUN X, et al. Sulforaphane protects Microcystin-LR-induced toxicity through activation of the Nrf2-mediated defensive response[J]. Toxicology and Applied Pharmacology, 2010, 247(2): 129-137.
[10]LIVINGSTONE D R. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms[J]. Marine Pollution Bulletin, 2001, 42(8): 656-666.
[11]GAUDIN J, HUET S, JARRY G, et al. In vivo DNA damage induced by the cyanotoxin microcystin-LR: Comparison of intra-peritoneal and oral administrations by use of the comet assay[J]. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2008, 652(1): 65-71.
[12]MA H, JIN M, ZHU T, et al. Effect of dietary arachidonic acid levels on growth performance, fatty acid profiles and lipid metabolism of juvenile yellow catfish (Pelteobagrus fulvidraco)[J]. Aquaculture, 2018, 486: 31-41.
[13]陈东星，王立改，楼宝，等. 饲料中维生素C添加量对黄姑鱼体组成成分和组织中抗氧化酶活力的影响[J]. 浙江海洋学院学报(自然科学版), 2016, 35(6): 472-477.
[14]中华人民共和国国家质量监督检验检疫总局.水体微囊藻毒素测定:GB/T 20466-2006 [S]. 北京: 中国标准出版社, 2006.
[15]中华人民共和国国家卫生和计划生育委员会.水产品中微囊藻毒素的测定:GB5009.273-2016 [S]. 北京: 中国标准出版社, 2016.
[16]DONG G, ZHU X, HAN D, et al. Response and recovery of hybrid sturgeon from subchronic oral administration of cyanobacteria[J]. Environmental Toxicology, 2011, 26(2): 161-170.
[17]SUN H, L K, MINTER E J A, et al. Combined effects of ammonia and microcystin on survival, growth, antioxidant responses, and lipid peroxidation of bighead carp Hypophthalmythys nobilis larvae[J]. Journal of Hazardous Materials, 2012, 221/222:213-219.
[18]熊达，张小雪，刘学，等. 茴三硫和硫辛酸对奥尼罗非鱼生长性能和抗氧化能力的影响[J]. 中国饲料, 2012(23): 28-31.
[19]武迪，管婷婷，张思雨，等. 维生素C对泥鳅幼鱼生长和免疫的影响[J]. 水产科学, 2016, 35(6): 658-662.
[20]XIE L, XIE P, GUO L, et al. Organ distribution and bioaccumulation of microcystins in freshwater fish at different trophic levels from the eutrophic Lake Chaohu, China[J]. Environmental Toxicology, 2005, 20(3): 293-300.
[21]姜锦林. 微囊藻毒素对典型水生生物的生态毒理效应及分子致毒机制研究[D]. 南京:南京大学, 2011.
[22]董桂芳，朱晓鸣，杨云霞，等. 黄颡鱼饲料中添加谷胱甘肽降低藻毒素毒性作用的研究[J]. 水生生物学报, 2010, 34(4): 722-730.
[23]张丽，许国焕，熊达，等. 罗非鱼饲料添加α-硫辛酸对藻毒素的解毒作用[J]. 淡水渔业, 2014, 44(3): 75-79.
[24]任胜杰，郑曙明，宋艳，等. 谷胱甘肽对异育银鲫生长及辛硫磷解毒的影响[J]. 西南农业学报, 2016, 29(12): 3009-3014.
[25]武晓会，刘洋，狄治朝，等. 低氧对斑马鱼细胞存活能力的影响[J].南方农业学报，2018, 49(8):1641-1647.
[26]张春云，曹丽萍，杜金梁，等. Nrf2介导姜黄素对四氯化碳诱导鲫鱼肝损伤的保护作用[J].江苏农业学报，2018,34(1):114-121.
[27]鲁耀鹏，陈晓丹，张秀霞，等. 饵料锰含量对凡纳滨对虾亚成虾生长及抗氧化活力的影响[J]. 南方农业学报，2018, 49(2): 391-396.
[28]PINHO G L L, MOURA DA ROSA C, MACIEL F E, et al. Antioxidant responses and oxidative stress after microcystin exposure in the hepatopancreas of an estuarine crab species[J]. Ecotoxicology and Environmental Safety, 2005, 61(3): 353-360.
[29]SAYEED I, PARVEZ S, PANDEY S, et al. Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch[J]. Ecotoxicology and Environmental Safety, 2003, 56(2): 295-301.
[30]安贸麟，范泽，王庆奎，等. 豆粕替代鱼粉对点带石斑鱼生长、消化和抗氧化能力的影响[J]. 江苏农业科学，2018，46(16): 128-132.
[31]计勇，陆光华. 污染水体的总抗氧化能力生物标志物研究[J]. 中国环境科学, 2010, 30(3): 395-399.
[32]ZHANG X, XIE P, LI D, et al. Time-dependent oxidative stress responses of crucian carp (Carassius auratus) to intraperitoneal injection of extracted microcystins[J]. Bulletin of Environmental Contamination and Toxicology, 2009, 82(5): 574-578.
[33]AMADO L L, GARCIA M L, PEREIRA T C B, et al. Chemoprotection of lipoic acid against microcystin-induced toxicosis in common carp (Cyprinus carpio, Cyprinidae)[J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2011, 154(3): 146-153.
[34]类延菊，徐玮，张彦娇，等. 水体铜胁迫条件下饲料中硫辛酸对皱纹盘鲍的保护作用[J]. 中国海洋大学学报(自然科学版), 2015, 45(7): 39-45.
[35]朱选，曹俊明，赵红霞，等. 饲料中添加谷胱甘肽对草鱼组织中谷胱甘肽沉积和抗氧化能力的影响[J]. 中国水产科学, 2008(1): 160-166.
[36]彭士明，施兆鸿，高权新，等. 增加饲料中VC质量分数对银鲳血清溶菌酶活性及组织抗氧化能力的影响[J]. 南方水产科学, 2013, 9(4): 16-21.

备注/Memo

备注/Memo:
收稿日期：2019-08-30基金项目：江苏省农业科技自主创新基金项目[ CX（18）2027]；国家水专项课题(2017ZX07203001)作者简介：徐文杰（1991-），女，山东泰安人，博士，助理研究员，主要从事富营养化水体生态修复研究。（E-mail）xuwenjieandbwb@163.com通讯作者：韩士群，（E-mail）shqunh@126.com
更新日期/Last Update: 2020-05-18