[1]王警,张雪寒,郭芸芸,等.江苏地区奶牛场blaNDM阳性大肠杆菌的分子流行病学[J].江苏农业学报,2020,(02):391-397.[doi:doi:10.3969/j.issn.1000-4440.2020.02.019]
　WANG Jing,ZHANG Xue-han,GUO Yun-yun,et al.Molecular epidemiological study on blaNDM-positive Escherichia coli in dairy farms in Jiangsu province[J].,2020,(02):391-397.[doi:doi:10.3969/j.issn.1000-4440.2020.02.019]
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江苏地区奶牛场blaNDM阳性大肠杆菌的分子流行病学()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
391-397
栏目:
畜牧兽医·水产养殖
出版日期:
2020-04-30

文章信息/Info

Title:
Molecular epidemiological study on blaNDM-positive Escherichia coli in dairy farms in Jiangsu province
作者:
王警1; 2; 张雪寒2; 郭芸芸1; 2; 张碧成2; 吴庆侠1; 何孔旺2
（1.西藏农牧学院动物科学学院，西藏林芝860000；2.江苏省农业科学院兽医研究所，江苏南京210014）
Author(s):
WANG Jing1; 2; ZHANG Xue-han2; GUO Yun-yun1; 2; ZHANG Bi-cheng2; WU Qing-xia1; HE Kong-wang2
(1.College of Animal Sciences, Tibet Agricultural and Animal Husbandry University, Linzhi 860000,China;2.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
NDM耐药基因; 大肠杆菌; 多位点序列分型; 药敏试验
Keywords:
drug-resistant gene NDM; Escherichia coli; multi-locus sequence typing(MLST); antimicrobial susceptibility testing
分类号:
S852.61+2
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.019
文献标志码:
A
摘要:
为了调查江苏地区奶牛场大肠杆菌中blaNDM基因的分子流行病学特征。从江苏部分地区3个奶牛场采集样品134份，使用PCR和测序的方法筛选blaNDM阳性大肠杆菌，同时对其进行超广谱β-内酰胺酶类基因的检测，采用药敏试验检测blaNDM阳性大肠杆菌对19种常用抗生素的敏感性，通过多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）对携带NDM基因的大肠杆菌进行亲缘关系分析，通过质粒结合试验验证blaNDM基因的可转移性。结果显示，从134份奶牛场样品中共分离15株blaNDM阳性大肠杆菌，包括NDM-1和NDM-5 2种变体。15株blaNDM阳性大肠杆菌对多种抗生素均呈现不同程度的耐药，奶牛场15株blaNDM阳性大肠杆菌包括7种ST型，分别是ST410、ST846、ST206、ST101、ST1642、ST3076、ST1626，同一种ST型在粪便样品和环境样品中同时存在，表明相同ST型blaNDM阳性大肠杆菌可在动物与环境间相互传播。PFGE试验结果表明，15株blaNDM阳性大肠杆菌可以分为11个簇，同一样品的分离株倾向于相同的簇，在比较小的范围内存在PFGE图谱相同的现象；对15株菌株的结合子进行blaNDM基因的转移性试验结果表明，15株大肠杆菌的blaNDM耐药基因均通过质粒结合转移至受体菌J53。表明，blaNDM阳性大肠杆菌在江苏省部分地区奶牛场中流行相对较为严重，存在多重耐药现象，推测blaNDM基因主要通过质粒结合转移的方式进行水平传播，这为食品源耐药菌的监测及风险评估提供了依据。
Abstract:
To investigate the epidemiology and molecular characteristics of blaNDM gene in Escherichia coli from dairy farms in Jiangsu province, a total of 134 samples were collected from three dairy farms in Jiangsu province. The blaNDM-positive E. coli was screened using PCR and sequencing, and the extended-spectrum β-lactamase gene was detected. The antimicrobial susceptibility testing was used to detect the sensitivity of blaNDM-positive E. coli to 19 commonly used antibiotics. The genetic relationship of E. coli carrying NDM gene was analyzed by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). The transferability of blaNDM gene was verified by conjugation experiments. The results showed that fifteen strains of blaNDM-positive E. coli were isolated from 134 dairy samples, including NDM-1 and NDM-5. All fifteen strains of blaNDM-positive E. coli exhibited different resistance to multiple antimicrobials and belonged to seven STs, namely ST410, ST846, ST206, ST101, ST1642, ST3076, ST1626. The same ST was involved in both fecal and environmental samples, indicating that blaNDM-positive E. coli with the same type of ST could be transmitted from animals to environment. The results of PFGE showed that fifteen strains of blaNDM-positive E. coli could be divided into 11 clusters, isolates of the same sample tended to have the same PFGE cluster, and the same PFGE pattern existed in a relatively small range. The blaNDM gene of fifteen strains of E. coli was transferred to receptor bacterium J53 by binding with plasmids. The blaNDM-positive E. coli is relatively more prevalent in dairy farms in Jiangsu, and there is multi-drug resistance. The blaNDM gene is mainly transmitted by means of combined transfer, which provides a basis for monitoring and risk assessment of drug-resistant bacteris.

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备注/Memo

备注/Memo:
收稿日期：2019-11-26基金项目：“十三五”国家重点研发计划项目（2018YFD0500504）作者简介：王警(1993-)，女，河南周口人，硕士研究生，主要从事食源性病原菌分离、鉴定和遗传进化分析。通讯作者：吴庆侠，（E-mail）goodwqx@163.com；何孔旺， （E-mail）kwh2003@263.net
更新日期/Last Update: 2020-05-18