[1]张奇,张振华,卢信.生物有机肥施用对黄泛冲积区贫瘠土壤养分、酶和微生物多样性的影响[J].江苏农业学报,2020,(02):325-335.[doi:doi:10.3969/j.issn.1000-4440.2020.02.011]
　ZHANG Qi,ZHANG Zhen-hua,LU Xin.Effects of bioorganic fertilizer application on nutrient, enzyme activity, microbial diversity of the barren soil in Yellow River alluvial areas[J].,2020,(02):325-335.[doi:doi:10.3969/j.issn.1000-4440.2020.02.011]
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生物有机肥施用对黄泛冲积区贫瘠土壤养分、酶和微生物多样性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
325-335
栏目:
耕作栽培·资源环境
出版日期:
2020-04-30

文章信息/Info

Title:
Effects of bioorganic fertilizer application on nutrient, enzyme activity, microbial diversity of the barren soil in Yellow River alluvial areas
作者:
张奇1; 2; 张振华2; 3; 卢信2; 3
(1.扬州大学环境科学与工程学院，江苏扬州225009；2.江苏省农业科学院农业资源与环境研究所，江苏南京210014；3.农业部长江下游平原农业环境重点实验室，江苏南京210014)
Author(s):
ZHANG Qi1; 2; ZHANG Zhen-hua2; 3; LU Xin2; 3
(1.College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, China;2.Institute of Agricultural Resources and Environmental Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture, Nanjing 210014, China)
关键词:
生物有机肥; 土壤养分; 土壤酶活; 土壤生物多样性
Keywords:
bio-organic fertilizer; soil nutrients; soil enzyme activity; soil microbial diversity
分类号:
S156.93
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.011
文献标志码:
A
摘要:
为了探究不同类型有机肥及施用量对黄泛冲积区贫瘠土壤的改良作用，以江苏省滨海县黄泛冲积区贫瘠土壤为供试土壤，研究了普通有机肥与生物有机肥分别在0 kg/hm2、2 250 kg/hm2、4 500 kg/hm2、6 750 kg/hm2施用量水平下对土壤的养分含量、土壤酶活性、生物多样性及玉米产量的影响。结果表明：在一个生长季内，相同施肥量水平下两种有机肥对土壤养分的作用效果无差异；苗期施用有机肥的土壤中速效养分含量较对照有所提升，且在一定范围内施肥量与土壤速效养分含量呈正相关关系。收获期各处理土壤蔗糖酶活性没有显著差异，但生物有机肥BOF2处理脲酶和碱性磷酸酶活性均较对照（CF）显著提高。土壤脲酶活性与碱解氮、速效钾、有机质含量呈显著正相关关系；土壤碱性磷酸酶活性与速效磷和速效钾含量呈显著正相关。施用普通有机肥及生物有机肥都可以提高土壤中微生物的丰富度，而生物有机肥的提升效果更为显著。生物有机肥在施用量2 250 kg/hm2及以上时可显著改变土壤微生物种群结构，而普通有机肥在施用量达6 750 kg/hm2时才会显著改变。生物有机肥BOF3（生物有机肥6 750 kg/hm2）处理与对照（CF）达到显著差异。可见，施用有机肥，特别是施用生物有机肥能改善黄泛冲积区贫瘠土壤养分性状，提高土壤酶活及土壤微生物多样性，最终实现作物增产的目的。
Abstract:
In order to investigate the effects of organic fertilizers on the improvement of the barren soil in Yellow River alluvial region, the soil in Binhai County, Jiangsu province was used as experimental soil. Effects of common organic and bioorganic fertilizer at different application levels (0 kg/hm2， 2 250 kg/hm2, 4 500 kg/hm2, 6 750 kg/hm2) on soil nutrient contents, enzyme activities, microbial diversity and maize yield were studied. The results showed that the application of two organic fertilizers produced similar effects on soil nutrients under the same application level in a growing season. The content of available nutrient in the soil applied with organic fertilizer at the seedling stage was higher than that of the control, and there was a positive correlation between the fertilizer amount and the content of available nutrient in a certain range. No significant difference was found in soil invertase activities during harvest stage, but the enzyme activities in the treatment of bio-organic fertilizer BOF2 were significantly higher than those in control. Urease activities were positively correlated with the contents of alkaline hydrolytic nitrogen, available potassium and organic matter. The alkaline phosphatase activities were significantly correlated with the contents of available phosphorus and available potassium. The application of organic fertilizer and bio-organic fertilizer increased the abundance of microorganisms in the soil, and the bio-organic fertilizer was more effective. When the application amount of bio-organic fertilizer was 2 250 kg/hm2 and above, the soil microbial population structure would be significantly changed. When the application amount of common organic fertilizer was 6 750 kg/hm2, the soil microbial population structure would be significantly changed. There was significant difference between the treatment of 6 750 kg/hm2 BOF3 and the control. It can be concluded that the application of organic fertilizer in the barren soil in Yellow River alluvial area, especially the application of bio-organic fertilizer, can significantly improve soil nutrients, increase soil enzyme activities and soil microbial diversity, and ultimately achieve the purpose of increasing crop production.

参考文献/References:

[1]张泽林. 浅析“18亿亩耕地红线”政策的现实意义[J]. 赤子(上中旬)，2017(3):162.
[2]宋雨婷.经济增长、城镇化与耕地非农化关系的计量分析——以江苏省为例[J].湖北农业科学，2017，56(15):2975-2978.
[3]李慧，王芳，赵庚星，等.黄泛平原区不同土地利用方式下的土壤养分状况分析[J].水土保持学报，2016，30(3):154-158.
[4]宁川川，王建武，蔡昆争.有机肥对土壤肥力和土壤环境质量的影响研究进展[J].生态环境学报，2016，25(1):175-181.
[5]王利辉. 不同来源有机肥及其配合施用对土壤性质的影响[D].长春:吉林农业大学,2007.
[6]周伟红. 有机肥对土壤培肥和作物产量的影响[D].长沙:湖南农业大学,2007.
[7]张运龙. 有机肥施用对冬小麦-夏玉米产量和土壤肥力的影响[D].北京:中国农业大学，2017.
[8]沈德龙.我国生物有机肥的发展现状及展望[J].中国土壤与肥料,2007(6):1-5.
[9]何蔚娟.生物有机肥料生产问题研究[J].陕西农业科学,2018,64(6):90-92.
[10]李婧.土壤有机质测定方法综述[J].分析试验室，2008(S1):154-156.
[11]张薇，付昀，李季芳，等.基于凯氏定氮法与杜马斯燃烧法测定土壤全氮的比较研究[J].中国农学通报，2015，31(35):172-175.
[12]钱君龙，张连弟，乐美麟.过硫酸盐消化法测定土壤全氮全磷[J].土壤，1990(5):258-262.
[13]王敏.碱熔-火焰光度法测定土壤全钾应注意的问题[J].辽宁林业科技，2016(6):75-76.
[14]夏栋，潘家荣，张博，等.土壤速效氮两种测定方法的测定值相关性研究[J].三峡大学学报(自然科学版)，2010，32(2):95-97.
[15]程乐明，陈良，刘建雷，等.碳酸氢钠浸提-钼锑抗比色法测定土壤有效磷的注意事项[J].现代农业科技，2009(3):205.
[16]郝卓敏.土壤速效钾的测定[J].昭乌达蒙族师专学报(自然科学版),2000(3):81-82.
[17]丰骁，段建平，蒲小鹏，等.土壤脲酶活性两种测定方法的比较[J].草原与草坪，2008(2):70-72.
[18]李朝英，郑路.基于DNS比色法的土壤蔗糖酶检测条件选择[J].中国农学通报，2016，32(27):171-176.
[19]石春芳，王志勇，冷小云，等.土壤磷酸酶活性测定方法的改进[J].实验技术与管理，2016，33(7):48-49.
[20]唐海滨，廖超英，刘莉丽，等.蔬菜大棚土壤脲酶、过氧化氢酶活性与土壤养分的关系[J].干旱地区农业研究，2011，29(3):165-168.
[21]李素俭，李新平，刘瑞丰,等.商洛药源基地土壤脲酶、磷酸酶和土壤养分的关系研究[J].水土保持学报，2010，24(6):227-230.
[22]PITTA D W, PARMAR N, PATEL A K, et al. Bacterial diversity dynamics associated with different diets and different primer pairs in the rumen of Kankrej cattle[J]. PLoS One, 2014, 9(11): e111710.
[23]SUN R B, ZHANG X X, GUO X S, et al. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw[J]. Soil Biology and Biochemistry, 2015, 88: 9-18.
[24]曹辉，李燕歌， 周春然，等. 炭化苹果枝对苹果根区土壤细菌和真菌多样性的影响[J]. 中国农业科学，2016，49(17): 3413-3424.
[25]鲁艳红. 长期施肥条件下红壤性水稻土有机质特征及其与土壤质量的关系[D].长沙:湖南农业大学,2011.
[26]王清奎，汪思龙，冯宗炜，等.土壤活性有机质及其与土壤质量的关系[J].生态学报，2005, 25 (3):513-519.
[27]郑曼迪,熊黑钢,乔娟峰,等. 基于综合光谱指数的不同程度人类干扰下土壤有机质含量预测[J]. 江苏农业学报,2018,34(5):1048-1056.
[28]刘效东，乔玉娜，周国逸.土壤有机质对土壤水分保持及其有效性的控制作用[J].植物生态学报，2011，35(12):1209-1218.
[29]单秀枝，魏由庆，严慧峻，等.土壤有机质含量对土壤水动力学参数的影响[J].土壤学报，1998, 35 (1):1-9.
[30]GREEN J L, HOLMES A J, WESTOBY M, et al. Spatial scaling of microbial eukaryote diversity[J].Nature, 2004, 432:747-750.
[31]NACHIMUTHU G, KING K, KRISTIANSEN P, et al. Comparison of methods for measuring soil microbial activity using cotton strips and a respirometer[J]. Journal of Microbiological Methods, 2007,69(2):322-329.
[32]王立刚,李维炯,邱建军,等.生物有机肥对作物生长、土壤肥力及产量的效应研究[J].土壤肥料,2004(5):12-16.
[33]刘国伟. 长期施用生物有机肥对土壤理化性质影响的研究[D].北京:中国农业大学,2004.
[34]林治安，赵秉强，袁亮，等.长期定位施肥对土壤养分与作物产量的影响[J].中国农业科学，2009，42(8):2809-2819.
[35]张德喜，吴卿. 不同耕作方式对农田土壤养分含量及土壤酶活性的影响[J]. 江苏农业科学，2018，46（11）：234-237.
[36]苏娜，杨丽娟，周崇俊，等.有机肥与氮肥配施对设施土壤中碱解氮含量的影响[J].安徽农业科学，2006(24):6542-6543.
[37]姬兴杰,熊淑萍,李春明,等.不同肥料类型对土壤酶活性与微生物数量时空变化的影响[J].水土保持学报, 2008, 22 (1) :123-133.
[38]章永松，林咸永，倪吾钟.有机肥对土壤磷吸附-解吸的直接影响[J].植物营养与肥料学报，1996, 2 (3):200-205.
[39]张亦驰，李林，史喜林，等.土壤钾素形态及有效性的研究进展[J].吉林农业科学，2013，38(6):52-54.
[40]李华，陈英旭，梁新强，等.土壤脲酶活性对稻田田面水氮素转化的影响[J].水土保持学报,2006, 20 (1):55-58.
[41]李琰琰，刘国顺，向金友，等.氮营养水平对植烟土壤养分含量及土壤酶活性的影响[J].中国烟草学报，2011，17(2):39-43.
[42]焦晓光，隋跃宇，张兴义.土壤有机质含量与土壤脲酶活性关系的研究[J].农业系统科学与综合研究，2008(4):494-496.
[43]田小明，李俊华，王成，等.连续3年施用生物有机肥对土壤养分、微生物生物量及酶活性的影响[J].土壤，2014，46(3):481-488.
[44]张建林，陆欣，王申贵.有机物料配比施用对土壤碱性磷酸酶活性的影响[J].土壤通报，2001(2):75-79.
[45]厉桂香，马克明.土壤微生物多样性海拔格局研究进展[J].生态学报，2018, 38(5): 1521-1529.
[46]金圣爱，黄少辉，刘建伟，等. 石灰氮-花生壳闷棚技术对设施土壤生物及黄瓜产量的影响[J]. 山东农业科学，2018, 50(1): 91-94.
[47]路璐，何燕. 不同林分土壤中氨氧化微生物的群落结构和硝化潜势差异及其驱动因子[J]. 南方农业学报，2018, 49(11): 2169-2176.
[48]李桥. 基于高通量测序技术下土壤微生物群落结构的研究[D].济南:山东师范大学，2014.
[49]孙波，赵其国，张桃林，等.土壤质量与持续环境 Ⅲ.土壤质量评价的生物学指标[J].土壤，1997(5):225-234.
[50]苏婷婷，徐宸，周鑫斌，等.生物有机肥对新整理烟田古菌群落组成的影响[J].中国烟草学报，2016，22(6):91-97.
[51]张丽梅，贺纪正.一个新的古菌类群——奇古菌门(Thaumarchaeota)[J].微生物学报，2012，52(4):411-421.
[52]PRESTON C M,WU K Y, MOLINSKI T F, et al．A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum [J]. Proceedings of the National Academy of Sciences of the United States of America,1996，93: 6241-6246．
[53]DORAN J W, SARRANTONIO M, LIEBIG M A. Soil health and sustainability[J]. Advances in Agronomy, 1996, 56(8):1-54.
[54]IBEKWE A M, KENNEDY A C, FROHNE P S, et al. Microbial diversity along a transect of agronomic zones[J]. FEMS Microbiology Ecology, 2002, 39(3):183-191.
[55]张迎春，颉建明，李静，等.生物有机肥部分替代化肥对莴笋及土壤理化性质和微生物的影响[J].水土保持学报，2019，33(4):196-205.

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备注/Memo:
收稿日期：2019-08-09基金项目：江苏省农业科技自主创新基金项目 [CX(17)1001-4]；国家重点研发计划项目（2018YFD0800306）作者简介：张奇（1993-），男，安徽安庆人，硕士，主要从事土壤改良与修复研究。 (E-mail)18752782205@163.com通讯作者：卢信，(Tel)025-84390581;(E-mail)lxdeng@126.com。张振华,(E-mail)zhenhuaz70@hotmail.com
更新日期/Last Update: 2020-05-18