[1]张娟琴,李双喜,郑宪清,等.蚯蚓防控西瓜枯萎病的效果及其机理探索[J].江苏农业学报,2020,(01):70-76.[doi:doi:10.3969/j.issn.1000-4440.2020.01.010]
　ZHANG Juan-qin,LI Shuang-xi,ZHENG Xian-qing,et al.Effects of earthworms on watermelon Fusarium wilt and its mechanism[J].,2020,(01):70-76.[doi:doi:10.3969/j.issn.1000-4440.2020.01.010]
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蚯蚓防控西瓜枯萎病的效果及其机理探索()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
70-76
栏目:
植物保护
出版日期:
2020-02-29

文章信息/Info

Title:
Effects of earthworms on watermelon Fusarium wilt and its mechanism
作者:
张娟琴1; 2; 3; 4; 5; 李双喜1; 2; 3; 4; 5; 郑宪清1; 2; 3; 4; 5; 张翰林1; 2; 3; 4; 5; 白娜玲1; 2; 3; 4; 5; 张海韵1; 2; 3; 4; 5; 吕卫光1; 2; 3; 4; 5
(1.上海市农业科学院生态环境保护研究所，上海201403；2.上海市设施园艺技术重点实验室，上海201403；3.上海市农业环境保护监测站，上海201403；4.农业部上海农业环境与耕地保育科学观测实验站，上海201403；5.上海低碳农业工程技术研究中心，上海201403)
Author(s):
ZHANG Juan-qin1; 2; 3; 4; 5;  LI Shuang-xi1; 2; 3; 4; 5; ZHENG Xian-qing1; 2; 3; 4; 5; ZHANG Han-lin1; 2; 3; 4; 5; BAI Na-ling1; 2; 3; 4; 5; ZHANG Hai-yun1; 2; 3; 4; 5; LYU Wei-guang1; 2; 3; 4; 5
（1.Eco-environmental Protection Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 2.Shanghai Key Laboratory of Horticultural Technology, Shanghai 201403, China;3.Environmental Protection Monitoring Station of Shanghai City, Shanghai 201403, China;4.Agricultural Environment and Farmland Conservation Experiment Station, Ministry of Agriculture, Shanghai 201403, China;5.Shanghai Low Carbon Agriculture Engineering Technology Research Center, Shanghai 201403, China)
关键词:
西瓜枯萎病; 蚯蚓; 西瓜专化型尖孢镰刀菌; 总酚酸; 土壤微生物量碳; 脱氢酶
Keywords:
watermelon Fusarium wilt; earthworm; Fusarium oxysporum f.sp. niveum; total phenolic acid; soil microbial biomass carbon; dehydrogenase
分类号:
S651;S436.5
DOI:
doi:10.3969/j.issn.1000-4440.2020.01.010
文献标志码:
A
摘要:
为了探明蚯蚓对西瓜枯萎病的影响机制，开拓西瓜枯萎病防控的新思路，本试验以西瓜连作土壤为基质，采用盆栽试验，研究不同密度的威廉环毛蚓（Pheretima guillelmi）和赤子爱胜蚓（Eisenia foetida）对西瓜长势、枯萎病发病率的影响，结合西瓜专化型尖孢镰刀菌数量、土壤微生物总量（土壤微生物量碳含量）、土壤微生物活性（土壤脱氢酶活性）以及土壤总酚酸的动态变化，探索蚯蚓对西瓜植株枯萎病的防控机理。结果表明，西瓜定植后45 d，试验组的西瓜蔓长、鲜质量较不投放蚯蚓对照分别增长了18.57%～67.27%、39.29%～87.24%。西瓜定植后30 d、45 d，对照的西瓜植株枯萎病发病率分别为62.15%、85.32%，显著高于蚯蚓试验组（P＜0.05）。在一定范围内增加蚯蚓密度能有效促进西瓜生长，减缓枯萎病发生。在蚯蚓质量相同的情况下，威廉环毛蚓的促生防病效果优于赤子爱胜蚓。0～45 d的试验过程中，试验组土壤中西瓜专化型尖孢镰刀菌（Fusarium oxysporum f.sp. niveum）数量、总酚酸含量较对照分别下降了36.63%～69.39%、31.0%～77.4%，45 d时试验组的土壤微生物量碳含量、土壤脱氢酶活性较对照分别提高了115.10～175.71 mg/kg、1.41～2.40 μg/(g·h)。蚯蚓能有效降低西瓜植株枯萎病的发病率，促进植株生长，其防控机理可能为：蚯蚓的穴居、取食等活动直接抑制土壤中尖孢镰刀菌的快速繁殖，同时蚯蚓通过降解土壤化感物质（总酚酸），提升微生物总量和活性等方式调控土壤微生物群落结构，改善土壤微生态环境，从而有效防控西瓜枯萎病的发生。
Abstract:
In order to explore the influence mechanism of Pheretima guillelmi and Eisenia foetida on watermelon Fusarium wilt and develop new ideas for the controlling， pot experiments were conducted to study the effects of different densities of Pheretima guillelmi and Eisenia foetida on watermelon vine length, fresh weight and incidence of Fusarium wilt. In addition, the control mechanism was explored by combining with the dynamic changes of Fusarium oxysporum f.sp. niveum, total phenolic acid, microbial biomass carbon and dehydrogenase activity in soil. The results showed that the vine length and fresh weight of watermelon in the experimental groups increased by 18.57%-67.27% and 39.29%-87.24% compared with those in control group at the 45th day after planting. At the 30th and 45th days, the wilt incidence of watermelon in the control group was 62.15% and 85.32%, respectively, which was significantly higher than that in the earthworm experimental groups（P＜0.05）. Increasing the earthworm density in a certain range could effectively promote the growth of watermelon and inhibit Fusarium wilt. Under the same weight, the growth and disease prevention effect of Pheretima guillelmi was better than that of Eisenia foetida. During the 0-45 days, the number of Fusarium oxysporum f.sp. niveum and total phenolic acid content in the soil of the earthworm experimental group decreased by 36.63%-69.39% and 31.0%-77.4%, respectively. At the 45th day, soil microbial biomass carbon and dehydrogenase activity in the earthworm experimental groups increased by 115.10-175.71 mg/kg and 1.41-2.40 μg/(g·h) compared with those in control group. Pheretima guillelmi and Eisenia foetida could effectively reduce the incidence of watermelon Fusarium wilt and promote plant growth. The mechanism may be that activities( burrowing and feeding) of earthworms directly inhibited the rapid propagation of Fusarium oxysporum in soil, and earthworms controlled soil microbial community structure by degrading soil allelochemicals (total phenols), increasing total microbial biomass and microbial activity. In conclusion, the soil micro-ecological environment was improved, and the occurrence of watermelon Fusarium wilt was effectively controlled.

参考文献/References:

[1]HUANG L F, SONG L X, XIA X J, et al. Plant-soil feedbacks and soil sickness: from mechanisms to application in agriculture[J]. Journal of Chemical Ecology, 2013, 39(2): 232-242.
[2]TESTE F P, KARDOL P, TURNER B L, et al. Plant-soil feedback and the maintenance of diversity in Mediterranean-climate shrublands[J]. Science, 2017, 355: 173-176.
[3]黄春艳,卜元卿,单正军,等.西瓜连作病害机理及生物防治研究进展[J].生态学杂志, 2016, 35(6): 1670-1676.
[4]吴风芝, 赵风艳, 刘元英.设施蔬菜连作障碍原因综合分析与防治措施[J].东北农业大学学报, 2000, 31(3): 241-247.
[5] 杨瑞平. 西瓜连作障碍缓解技术及其机理研究[D]. 杨凌：西北农林科技大学, 2016.
[6] 王 斌, 李 根, 陈 欢, 等.蚯蚓作用下土壤化学组成和性状的动态变化[J].水土保持学报,2013,27(3):273-277.
[7]DORAN J W, ZEISS M R. Soil health and sustainability: managing the biotic component of soil quality[J]. Applied Soil Ecology, 2000, 15: 3-11.
[8] EDWARDS C A, FLETCHER K E. Interactions between earthworms and microorganisms in organic-matter breakdown[J]. Agriculture, Ecosystems & Environment, 1988, 24(1/3): 235-247.
[9]毕艳孟,孙振钧.蚯蚓调控土壤微生态缓解连作障碍的作用机制[J].生物多样性,2018,26(10):1103-1115.
[10]SZCZECH M M. Suppressiveness of vermicompost against Fusarium wilt of tomato[J]. Journal of Phytopathology, 1999, 147(3): 155-161.
[11]柏彦超, 周雄飞, 赵学辉,等. 蚓粪基质克服西瓜连作障碍的应用效果研究[J]. 中国农学通报, 2011, 27(8): 212-216.
[12]吴盼盼. 蚓粪对设施内番茄连作土壤微生物区系的调节[D].沈阳：沈阳农业大学, 2016.
[13]毕艳孟. 蚯蚓缓解草莓连作障碍的效应及机理研究[D].北京：中国农业大学, 2016.
[14]徐 琴, 肖能文. 中国陆栖蚯蚓[M]. 北京: 中国农业出版社, 2010: 54-68.
[15]郭书普. 西瓜、甜瓜病虫害鉴别与防治技术图解［M］.北京：化学工业出版社，2012：15-27.
[16]赵 爽, 罗 佳, 凌 宁, 等. 基因宏阵列和荧光定量PCR方法对西瓜枯萎病害土壤中尖孢镰刀菌的快速检测和定量[J]. 土壤学报, 2010, 47(4): 703-708.
[17]LI L J, HAN X Z. Changes of soil properties and carbon fractions after long-term application of organic amendments in Mollisols[J]. Catena, 2016,143:140-144.
[18]CASIDA JR L E, KLEIN D A, SANTORO T. Soil dehydrogenase activity[J]. Soil Science, 1964, 98(6): 371-376.
[19]BOX J D. Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters[J]. Water Research, 1983, 17(5): 511-525.
[20]CAO J, WANG C, HUANG Y. Interactive impacts of earthworms (Eisenia fetida) and arbuscular mycorrhizal fungi (Funneliformis mosseae) on the bioavailability of calcium phosphates[J]. Plant and Soil, 2015, 396(1/2): 45-57.
[21]LI H, WANG C, LI X, et al. Inoculating maize fields with earthworms (Aporrectodea trapezoides) and an arbuscular mycorrhizal fungus (Rhizophagus intraradices) improves mycorrhizal community structure and increases plant nutrient uptake[J]. Biology and Fertility of Soils, 2013, 49(8): 1167-1178.
[22]WU D, LIU M, SONG X, et al. Earthworm ecosystem service and dis-service in an N-enriched agroecosystem: Increase of plant production leads to no effects on yield-scaled N2O emissions[J]. Soil Biology and Biochemistry, 2015, 82: 1-8.
[23]曾令涛, 王东升, 常江杰, 等. 蚯蚓堆肥与益生菌配施对西瓜生长和品质的影响[J].中国土壤与肥料,2017(1):103-110.
[24]MAZZOLA M, MANICI L M. Apple replant disease: role of microbial ecology in cause and control[J]. Annual Review of Phytopathology, 2012, 50: 45-65.
[25]马宁宁, 李天来. 设施番茄长期连作土壤微生物群落结构及多样性分析[J]. 园艺学报, 2013, 40(2):255-264.
[26]SHAN J, LIU J, WANG Y, et al. Digestion and residue stabilization of bacterial and fungal cells, protein, peptidoglycan, and chitin by the geophagous earthworm Metaphire guillelmi[J]. Soil Biology and Biochemistry, 2013, 64: 9-17.
[27]张宝贵.蚯蚓与微生物的相互作用[J].生态学报,1997，17(5):556-560.
[28]BI Y M, TIAN G L, WANG C, et al. Differential effects of two earthworm species on Fusarium wilt of strawberry[J]. Applied Soil Ecology, 2018, 126: 174-181.
[29]李 欢, 杜志勇, 刘 庆,等. 蚯蚓菌根互作对土壤酶活、甘薯根系生长及养分吸收的影响[J]. 植物营养与肥料学报, 2016, 22（1）: 209-215.
[30]ZHANG B G, LI G T, SHEN T S, et al. Changes in microbial biomass C, N, and P and enzyme activities in soil incubated with the earthworms Metaphire guillelmi or Eisenia fetida[J]. Soil Biology and Biochemistry, 2000, 32(14): 2055-2062.
[31]GMEZ-BRANDN M, AIRA M, LORES M, et al. Epigeic earthworms exert a bottleneck effect on microbial communities through gut associated processes[J]. PLoS ONE, 2011, 6(9): e24786.
[32]VAITAUSKIENE K, ARAUSKIS E, NAUJOKIENE V, et al. The influence of free-living nitrogen-fixing bacteria on the mechanical characteristics of different plant residues under no-till and strip-till conditions[J]. Soil and Tillage Research, 2015, 154: 91-102.
[33]ZHANG N, WANG D, LIU Y, et al. Effects of different plant root exudates and their organic acid components on chemotaxis, biofilm formation and colonization by beneficial rhizosphere-associated bacterial strains[J]. Plant and Soil, 2014, 374(1/2): 689-700.
[34]BI Y M, TIAN G L, WANG C, et al. Application of leaves to induce earthworms to reduce phenolic compounds released by decomposing plants[J]. European Journal of Soil Biology, 2016, 75: 31-37.
[35]JANVIER C, VILLENEUVE F, ALABOUVETTE C, et al. Soil health through soil disease suppression: which strategy from descriptors to indicators?[J]. Soil Biology and Biochemistry, 2007, 39(1): 1-23.

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备注/Memo

备注/Memo:
收稿日期：2019-06-03基金项目：上海市农口青年成长计划项目[沪农青字（2017）第1-21号]；上海市科技兴农重大项目[沪农科推字(2018第4-14号）]；上海市农业科学院卓越团队建设计划[农科创2017(A-03)]；上海市科委项目(18295810500、16391901500）作者简介：张娟琴（1982-），女，山西吉县人，硕士，助理研究员，主要从事环境监测及土壤改良研究。(E-mail)zhjuanqin501@163.com通讯作者：吕卫光，(E-mail)lwei1217@si
更新日期/Last Update: 2020-03-13