[1]田孟祥,张时龙,何友勋,等.水稻耐低温基因bZIP73分子标记的开发与验证[J].江苏农业学报,2019,(06):1265-1270.[doi:doi:10.3969/j.issn.1000-4440.2019.06.001]
　TIAN Meng-xiang,ZHANG Shi-long,HE You-xun,et al.Development and verification of molecular markers of chilling tolerance gene bZIP73 in rice[J].,2019,(06):1265-1270.[doi:doi:10.3969/j.issn.1000-4440.2019.06.001]
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水稻耐低温基因bZIP73分子标记的开发与验证()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年06期
页码:
1265-1270
栏目:
遗传育种·生理生化
出版日期:
2019-12-31

文章信息/Info

Title:
Development and verification of molecular markers of chilling tolerance gene bZIP73 in rice
作者:
田孟祥1; 张时龙1; 何友勋1; 余本勋1; 余莉1; 宫彦龙2; 雷月2; 张大双2; 叶永印1
（1.毕节市农业科学研究所，贵州毕节551714；2.贵州省水稻研究所，贵州贵阳550006）
Author(s):
TIAN Meng-xiang1; ZHANG Shi-long1; HE You-xun1; YU Ben-xun1; YU Li1; GONG Yan-long2; LEI Yue2; ZHANG Da-shuang2; YE Yong-yin1
(1.Bijie Institute of Agricultural Sciences, Bijie 551714, China;2.Guizhou Rice Institute, Guiyang 550006, China)
关键词:
水稻; 耐低温; bZIP73基因; 分子标记
Keywords:
rice; chilling tolerance; bZIP73 gene; molecular marker
分类号:
S511.032
DOI:
doi:10.3969/j.issn.1000-4440.2019.06.001
文献标志码:
A
摘要:
为能够对耐低温基因bZIP73的不同类型进行准确鉴别，根据粳稻bZIP73Jap与籼稻bZIP73Ind在编码区的一个碱基差别，开发了由4条引物组成的分子标记，并应用7份籼稻、7份粳稻及3份籼粳杂交的F1材料对分子标记进行检测验证。电泳检测图显示，粳稻bZIP73Jap扩增出681 bp和342 bp 2种条带，籼稻bZIP73Ind扩增出681 bp和387 bp 2种条带，而籼粳杂合子F1则扩增出681 bp、342 bp及387 bp 3种条带，所有材料的扩增条带大小与预测目的片段均一致，说明所开发出的分子标记能对基因bZIP73的不同类型进行准确判断。该方法具有费用低廉、操作简便、快速高效等优点，可在资源鉴定和育种中推广应用。
Abstract:
In order to accurately identify the different types of chilling tolerance gene bZIP73, a molecular marker consisting of four primers was developed according to the difference of single base in coding region between japonica bZIP73Jap and indica bZIP73Ind. The molecular markers were tested by seven indica rice, seven japonica rice and three indica-japonica hybrid F1 materials. Electrophoretic detection results showed that 681 bp and 342 bp bands were amplified from bZIP73Jap, 681 bp and 387 bp bands were amplified from bZIP73Ind, and 681 bp, 342 bp and 387 bp bands were amplified from indica and japonica heterozygote F1. The size of amplified bands of all rice materials was consistent with that of the predicted target fragments, indicating that the developed molecular markers could accurately judge the different types of gene bZIP73. This method has the advantages of low cost, simple operation, fast and high efficiency, and can be widely used in resource identification and breeding.

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备注/Memo

备注/Memo:
收稿日期：2019-07-17 基金项目：国家重点研发计划项目（2017YFD0100205）；贵州省科技成果应用及产业化计划项目[黔科合成果(2018)4306]；毕节试验区特色优势作物资源研发平台建设黔科合条中补地项目[(2015)4003]；毕节市科技支撑计划项目[毕科合字(2016)20号] 作者简介：田孟祥（1983-），男，贵州麻江人，硕士，助理研究员，主要从事水稻育种及栽培研究。(E-mail) tmengxiang@126.com 通讯作者：张时龙，(E-mail)bjrice@163.com
更新日期/Last Update: 2020-01-09