[1]叶旻硕,俞键烽,马艳,等.不同微生物菌剂对辣椒疫病的防控效果及对土壤性状的影响[J].江苏农业学报,2019,(04):811-817.[doi:doi:10.3969/j.issn.1000-4440.2019.04.009]
 YE Min shuo,YU Jian feng,MA Yan,et al.Effects of different microbial inoculants on pepper blight suppression and soil properties[J].,2019,(04):811-817.[doi:doi:10.3969/j.issn.1000-4440.2019.04.009]
点击复制

不同微生物菌剂对辣椒疫病的防控效果及对土壤性状的影响()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
811-817
栏目:
植物保护
出版日期:
2019-08-31

文章信息/Info

Title:
Effects of different microbial inoculants on pepper blight suppression and soil properties
作者:
叶旻硕1俞键烽1马艳2黄有军1
(1.浙江农林大学林业与生物技术学院,浙江杭州311300;2.江苏省农业科学院农业资源与环境研究所,江苏南京210014)
Author(s):
YE Minshuo1YU Jianfeng1MA Yan2HUANG Youjun1
(1.School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;2.Institute of Agricultural Resource and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
辣椒疫病微生物菌剂土壤微生物定殖辣椒疫霉
Keywords:
pepper blightmicrobial inoculantssoil microorganismcolonizationPhytophthora capsici
分类号:
S432.4
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.009
文献标志码:
A
摘要:
采用盆栽试验的方法,研究解淀粉芽孢杆菌(BB2)、长枝木霉(TB2)、棘孢曲霉(BAS1)3种微生物菌剂对辣椒疫病的防控效果以及对土壤性状的影响。通过测定病情指数、植株生物量、土壤化学及土壤微生物等指标,研究不同处理下辣椒疫病的发生程度以及相应土壤性状的变化特征。研究结果表明,3种微生物菌剂均有一定的防控效果;辣椒移栽前期,BAS1处理从第13 d开始病情指数增长较快,第19 d后增长逐渐平缓,而BB2、TB2处理则从第25 d起病情指数开始加速增长;移栽后40 d,各处理的防治效果从高到低依次为BB2、TB2、BAS1,其中BB2的防治效果最高,达到了60%以上。土壤微生物数量测定结果显示,3种微生物菌剂处理后,土壤中相应微生物的数量显著高于其他处理,而辣椒疫霉的数量显著低于对照,表明3种微生物在辣椒根际均有较好的定殖作用,且能抑制病原菌的增殖。
Abstract:
The biocontrol effect of Bacillus amyloliquefaciens(BB2), Trichoderma longibrachiatum(TB2), and Aspergillus aculeatus(BAS1) on pepper blight caused by Phytophthora capsici was investigated through pot experiment. By measuring disease index, plant biomass, soil chemical properties and soil microorganism, the occurrence degree and the variation characteristics of corresponding soil properties under different treatments were studied. The pot experiment suggested that all the three microbial inoculants showed disease inhibition effect on pepper blight. In the early stage of pepper transplanting, the disease index increased rapidly from the isth day of BAS1 treatment, and gradually slowed down from the 19th day. However, the disease index increased rapidly from the 25th day of BB2 and TB2 treatments. The BB2 treatment showed the highest preventive efficiency, which reached to 60%, followed by TB2 and on the 40th day after transplanting. The number of corresponding microorganisms in the three microbial agents treatments was significantly higher than that in the other treatments, while the number of Phytophthora capsici was significantly lower than that in the control, indicating that the three microorganisms had good colonization in the rhizosphere of pepper, and could inhibit the proliferation of pathogens.

参考文献/References:

[1]KIM K D, NEMEC S, MUSSON G. Effects of composts and soil amendments on soil microflora and Phytophthora root and crown rot of bell pepper[J]. Crop Protection, 1997, 16(2): 165-172.
[2]周涛, 罗路云, 陈红松,等. 辣椒疫病罹病植株根际土壤细菌群落多样性分析[J]. 南方农业学报,2017,48(6):1014-1018.
[3]张世才,熊艳,黄任中,等.重庆地区辣椒疫霉菌的分离培养及生理小种鉴定[J].植物保护,2015,41(3):183-187.
[4]杨叔青,胡栓红,杨志刚,等.不同地区辣椒疫霉菌生理小种的鉴定和生物学特性的研究[J].华北农学报,2015,30(2):104-109.
[5]薛春生,何瑞玒,肖淑芹,等.辽宁省辣椒疫霉菌生理小种的生物学特性及生物制剂对辣椒疫病的防效[J].植物保护学报,2017,44(4):650-656.
[6]王新生,卢惠芝,周俊.5种杀菌剂防治辣椒疫病田间药效试验[J].农药科学与管理,2009,30(4):34-35.
[7]JANG Y, YANG E, CHO M, et al. Effect of grafting on growth and incidence of phytophthora blight and bacterial wilt of pepper (Capsicum annuum L.)[J]. Horticulture Environment & Biotechnology, 2012, 53(1): 9-19.
[8]FRANK J L, RIVARD C L, KUBOTA C. Grafting fruiting vegetables to manage soilborne pathogens, foliar pathogens, arthropods and weeds[J]. Scientia Horticulturae, 2010, 127(2): 127-146.
[9]吴石平,袁洁,杨学辉,等.几种杀菌剂对辣椒疫病的抑菌活性研究[J].安徽农业科学,2009,37(1):211-212.
[10]苏荣存.嫁接栽培对辣椒产量和抗病性的影响[J].北方园艺,2012(1):43-44.
[11]杨茹薇,秦勇,吴慧,等.辣椒嫁接抗疫病效果研究[J].新疆农业大学学报,2010,33(1):27-30.
[12]席亚东,陈国华,谢丙炎,等.辣椒疫霉菌全球传播与危害及生物学特性研究进展[J].北方园艺,2016(11):199-203.
[13]何延静,刘海明,胡洪波,等.一株拮抗辣椒疫霉的假单胞菌的分离与鉴定[J].微生物学报,2006,46(4):516-521.
[14]韩长志.植物病原拮抗菌木霉属真菌的研究进展[J].江苏农业学报,2016,32(4):946-952.
[15]李松鹏,崔琳琳,程家森,等.两株哈茨木霉菌株防治水稻纹枯病及促进水稻生长的潜力研究[J].植物病理学报,2018,48(1):98-107.
[16]覃柳燕, 郭成林, 黄素梅,等. 棘孢木霉菌株PZ6对香蕉促生效应及枯萎病室内防效的影响[J]. 南方农业学报,2017,48(2):277-283.
[17]张阳,刘重喜,王相晶,等.微生物代谢产物在植物病害防治中的应用[J].世界农药,2013,35(3):34-40.
[18]曹葳蕤,薛晓锋,吴黎明.烟曲霉素及其防治蜜蜂微孢子虫病的研究进展[J].中国农业科技导报,2016,18(2):52-58.
[19]劳家柽.土壤农化分析手册[M].北京:农业出版社,1988.
[20]DRIGO B, VEEN J V, KOWALCHUK G A. Specific rhizosphere bacterial and fungal groups respond differently to elevated atmospheric CO2[J]. ISME Journal, 2009, 3(10): 1204-1217.
[21]WANG Q, MA Y, WANG G, et al. Integration of biofumigation with antagonistic microorganism can control Phytophthora blight of pepper plants by regulating soil bacterial community structure[J]. European Journal of Soil Biology, 2014, 61(5): 58-67.
[22]GOEBES M D, HILDEMANN L M, KUJUNDZIC E, et al. Realtime PCR for detection of the Aspergillus genus[J]. Journal of Environmental Monitoring JEM, 2007, 9(6): 599-609.
[23]郝楠,仝赞华,邱德文.侧孢短芽孢杆菌A60的筛选及其对辣椒疫霉的室内防效测定[J].生物技术通报,2017(9):160-165.
[24]汪涛,迟元凯,赵伟,等.多粘类芽孢杆菌TC35的鉴定及对辣椒疫病的田间防效[J].安徽农业科学,2018,581(4):138-139.
[25]杨威,闫海霞,张贝贝,等.施用微生物菌肥“宁盾”对辣椒根围细菌多样性及土壤酶活性的影响[J].江苏农业科学,2018,46(9):99-103.
[26]席亚东,陈国华,谢丙炎,等.不同木霉菌株对辣椒疫霉菌的防控作用[J].北方园艺,2016(21):115-119.
[27]王宁,刘铜,靳亚忠,等.木霉菌对土壤微生物多样性及草莓生长和发病的影响[J].江苏农业科学,2018,46(18):108-112.
[28]胡琼,张海松.木霉菌TC对辣椒疫病的防治效果[J].北方园艺,2012(3):147-149.
[29]梁军锋.辣椒疫病生防菌的防病促生效应、作用机制及应用研究[D].杨凌:西北农林科技大学,2006.

相似文献/References:

[1]王秋君,常志州,王光飞,等.铜绿假单胞菌结合生物熏蒸防控辣椒疫病的效果[J].江苏农业学报,2015,(02):290.[doi:10.3969/j.issn.1000-4440.2015.02.011]
 WANG Qiu-jun,CHANG Zhi-zhou,WANG Guang-fei,et al.Integration of Pseudomonas aeruginosa with biofumigation to control phytophthora blight of pepper[J].,2015,(04):290.[doi:10.3969/j.issn.1000-4440.2015.02.011]
[2]朱丽,王庆莲,唐山远,等.不同试剂处理对草莓植株生长及根际微生物群落结构的影响[J].江苏农业学报,2023,(01):198.[doi:doi:10.3969/j.issn.1000-4440.2023.01.023]
 ZHU Li,WANG Qing-lian,TANG Shan-yuan,et al.Effects of different reagent treatments on strawberry plant growth and rhizosphere microbial community structure[J].,2023,(04):198.[doi:doi:10.3969/j.issn.1000-4440.2023.01.023]
[3]张金辉,李银月,张发文,等.物料碳氮比及微生物菌剂接种量对黄瓜秧-鸡粪堆肥过程的影响[J].江苏农业学报,2024,(02):260.[doi:doi:10.3969/j.issn.1000-4440.2024.02.008]
 ZHANG Jin-hui,LI Yin-yue,ZHANG Fa-wen,et al.Effects of carbon-nitrogen ratio of materials and inoculation amount of microbial inoculants on the composting process of cucumber seedlings-chicken manure[J].,2024,(04):260.[doi:doi:10.3969/j.issn.1000-4440.2024.02.008]

备注/Memo

备注/Memo:
收稿日期:2018-10-18 基金项目:国家重点研发计划项目(2016YFD0201007);江苏省重点研发计划项目(现代农业)(BE2017379-1) 作者简介:叶旻硕(1993-),男,浙江杭州人,硕士研究生,主要从事设施园艺作物的研究。(E-mail)345468519@qq.com 通讯作者:黄有军,(E-mail)hyj@zafu.edu.cn
更新日期/Last Update: 2019-08-31