[1]朱守晶,史文娟.苎麻镉响应基因BnG6PDH1的克隆和表达分析[J].江苏农业学报,2019,(02):262-270.[doi:doi:10.3969/j.issn.1000-4440.2019.02.004]
　ZHU Shou-jing,SHI Wen-juan.Cloning and expression analysis of cadmium-responsive gene BnG6PDH1 from ramie (Boehmeria nivea L.)[J].,2019,(02):262-270.[doi:doi:10.3969/j.issn.1000-4440.2019.02.004]
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苎麻镉响应基因BnG6PDH1的克隆和表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
262-270
栏目:
遗传育种·生理生化
出版日期:
2019-04-30

文章信息/Info

Title:
Cloning and expression analysis of cadmium-responsive gene BnG6PDH1 from ramie (Boehmeria nivea L.)
作者:
朱守晶1; 2; 史文娟1
（1.宜春学院生命科学与资源环境学院，江西宜春336000；2.江西省作物生长发育调控重点实验室，江西宜春336000）
Author(s):
ZHU Shou-jing1; 2; SHI Wen-juan1
(1.College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China;2.Jiangxi Key Laboratory of Regulation and Control of Crop Growth and Development , Yichun 336000, China)
关键词:
苎麻; 葡萄糖-6-磷酸脱氢酶; 镉响应基因BnG6PDH1; 基因克隆; 表达分析
Keywords:
Boehmeria nivea; glucose-6-phosphate dehydrogenase; cadmium-responsive gene BnG6PDH1; gene clone; expression analysis
分类号:
Q786
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.004
文献标志码:
A
摘要:
葡萄糖-6-磷酸脱氢酶（G6PDH）是磷酸戊糖代谢途径的关键限速酶，为探讨G6PDH基因在苎麻耐镉机制中的作用，本研究在苎麻镉胁迫转录组的基础上，采用RACE方法从苎麻（Boehmeria nivea L.）品种中苎一号中克隆到BnG6PDH1基因（GenBank登录号为MG941010）的全长cDNA序列，该基因开放读码框为1 554 bp，编码517个氨基酸，蛋白质分子量为59 250，等电点为5.92。BnG6PDH1编码的氨基酸与川桑（XP_010111634.1）、枣（XP_015878928.1）、甜樱桃（XP_021825040.1）、苹果（XP_008362117.1）和梅（XP_008231184.1）的胞质型G6PDH蛋白的相似性较高，相似度分别为92%、91%、89%、88%和88%。组织表达特异性分析结果表明，BnG6PDH1在苎麻叶中的表达量最高，其次为茎，根部的表达量最低。镉胁迫诱导表达分析结果表明，BnG6PDH1的显著表达受镉的诱导，表达量随着镉质量浓度的增加而增加。以上结果表明，BnMYB1是一个镉应答基因，可能在植物对镉胁迫的适应中发挥重要作用。
Abstract:
Glucose-6-phosphate dehydrogenase is a main regulatory enzyme for pentose phosphate pathway. In this paper, a G6PDH gene named BnG6PDH1 (GenBank accession: MG941010) was isolated from ramie (Boehmeria nivea L.) by amplification of cDNA ends (RACE) based on the analysis of expression profiling of cadmium response genes in ramie. The open reading frame of BnG6PDH1 was 1 554 bp, and 517 amino acid residues were encoded. The predicted protein was 59 250 with isoelectric point of 5.92. The similarity comparison revealed that the deduced amino acid sequence shared 92%, 91%, 89%, 88% and 88% of similarity with Morus notabilis (XP_010111634.1), Ziziphus jujuba (XP_015878928.1), Prunus avium (XP_021825040.1), Malus domestica (XP_008362117.1) and Prunus mume (XP_008231184.1). The results of specificity analysis showed that the expression of BnG6PDH1 was in leaf and the lowest in root. Furthermore, BnG6PDH1 gene was significantly induced by cadmium stress, and the expression of BnG6PDH1 increased with the increase of Cd2+ concentration. Collectively, the results suggest that BnG6PDH1 is a cadmium-responsive factor and may play an important role in the plant adaption to cadmium stress.

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备注/Memo

备注/Memo:
收稿日期：2018-06-26 基金项目：江西省教育厅科学技术研究项目（GJJ161008）；江西省作物生长发育调控重点实验室开放基金项目（KFJJ201703） 作者简介：朱守晶（1985-），男，安徽淮南人，博士，讲师，主要从事苎麻耐镉分子机理研究。（E-mail）zhusj85@sina.com
更新日期/Last Update: 2019-05-05