[1]练旺民,徐薇,孟卓玲,等.表油菜素内酯在缓解水稻砷毒害中的作用[J].江苏农业学报,2018,(06):1267-1275.[doi:doi:10.3969/j.issn.1000-4440.2018.06.010]
　LIAN Wang-min,XU Wei,MENG Zhuo-ling,et al.Effects of supplementation of epibrassinolide on alleviation of arsenic stress in rice[J].,2018,(06):1267-1275.[doi:doi:10.3969/j.issn.1000-4440.2018.06.010]
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表油菜素内酯在缓解水稻砷毒害中的作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年06期
页码:
1267-1275
栏目:
耕作栽培·资源环境
出版日期:
2018-12-25

文章信息/Info

Title:
Effects of supplementation of epibrassinolide on alleviation of arsenic stress in rice
作者:
练旺民; 徐薇; 孟卓玲; 陶之欢; 金珂; 郑晶; 张佳艺; 刘庆坡
（浙江农林大学农业与食品科学学院，浙江临安311300）
Author(s):
LIAN Wang-min; XU Wei; MENG Zhuo-ling; TAO Zhi-huan; JIN Ke; ZHENG Jing; ZHANG Jia-yi; LIU Qing-po
(School of Agriculture and Food Science, Zhejiang A&F University, Lin′an 311300, China)
关键词:
水稻; 砷; 表油菜素内酯; 基因表达
Keywords:
rice; arsenic; epibrassinolide; gene expression
分类号:
S511.01
DOI:
doi:10.3969/j.issn.1000-4440.2018.06.010
文献标志码:
A
摘要:
以砷耐受性中等的籼稻品种93-11为材料，通过营养液水培,生理指标测定和基因表达分析等方法，研究了表油菜素内酯在缓解水稻亚砷酸盐毒害中的作用。结果表明，外施表油菜素内酯能有效促进受砷毒害水稻幼苗的生长，降低水稻根系的砷积累量，减少砷胁迫对根系细胞膜透性的影响，降低脯氨酸和非蛋白巯基化合物含量以及叶片中H2O2和O2·-等活性氧自由基的含量。通过定量表达分析发现，外施表油菜素内酯可降低水稻根系中亚砷酸盐和砷酸盐吸收相关基因的表达，但显著提高了砷酸还原酶基因的表达。说明外施表油菜素内酯可在一定程度上缓解砷对水稻的毒害作用。
Abstract:
Using 93-11, an indica rice cultivar with moderate arsenite (AsIII) tolerance as material, the potential role of external epibrassinolide (EBR) in regulation of AsIII tolerance in rice was investigated, using combinations of hydroponic culture, physiological indices measurements, and gene expression analysis. The results showed that compared with the addition of AsIII alone, supplementation of external EBR when seedlings being exposed to AsIII, the growth and cell membrane permeability of seedlings were extensively recovered, in the meantime, the accumulation of arsenic in rice seedlings was decreased. In addition, it was observed that after addition of EBR, the contents of proline, non-thiol protein, reactive oxygen species including H2O2 and O2·- were extensively decreased. Real-time RT-PCR revealed that after supplementation of external EBR under AsIII stress, the expression level of genes involved in arsenite and arsenate uptake and translocation were strongly repressed, while the expression of arsenate reductase was markedly induced. In conclusion, the supplementation of external EBR plays a positive role in alleviating AsIII stress in rice.

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备注/Memo

备注/Memo:
收稿日期：2018-08-09 基金项目：国家自然科学基金面上项目（31471431）；国家级大学生创新创业训练计划项目（2013200068）；浙江省自然科学基金重点项目（LZ19B070001）；浙江农林大学学生科研训练项目（2013200037） 作者简介：练旺民（1996-），男，浙江丽水人，本科，研究方向为作物逆境生物学。(E-mail)979363065@qq.com。徐薇为共同第一作者。 通讯作者：刘庆坡，(E-mail)liuqp@zafu.edu.cn
更新日期/Last Update: 2018-12-28