[1]王恒波,余泽怀,肖乃衍,等.转基因番木瓜检测方法的建立[J].江苏农业学报,2018,(05):1198-1200.[doi:doi:10.3969/j.issn.1000-4440.2018.05.032]
 WANG Heng-bo,YU Ze-huai,XIAO Nai-yan,et al.Establishment of detection method for transgenic papaya[J].,2018,(05):1198-1200.[doi:doi:10.3969/j.issn.1000-4440.2018.05.032]
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转基因番木瓜检测方法的建立()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1198-1200
栏目:
研究简报
出版日期:
2018-10-25

文章信息/Info

Title:
Establishment of detection method for transgenic papaya
作者:
王恒波12余泽怀3肖乃衍12张华12陈平华12
(1.福建农林大学国家甘蔗工程技术研究中心,福建福州350002;2.农业部甘蔗及制品质量监督检验测试中心转基因检测室,福建福州350002;3.福建农林大学金山学院,福建福州350002)
Author(s):
WANG Heng-bo12YU Ze-huai3XIAO Nai-yan12ZHANG Hua12CHEN Ping-hua12
(1.National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture, Fuzhou 350002, China;3.Jinshan College of Fujian Agriculture and Forestry University, Fuzhou 350002, China)
关键词:
转基因番木瓜筛选定性聚合酶链式反应(PCR)检测
Keywords:
transgenic papayascreeningqualitative polymerase chain reaction (PCR)detection
分类号:
S667.9
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.032
文献标志码:
A

参考文献/References:

[1]高艳梅,翟金玲,徐远峰,等. 抗PRSV转基因番木瓜研究进展[J]. 安徽农学通报, 2009, 15(3):36-38.
[2]张雨良,黄启星,朱丽娣,等. 海南地区番木瓜花叶病病原的分子鉴定与多样性分析[J]. 植物研究, 2014, 34(5):694-699.
[3]饶雪琴,李华平. 转基因番木瓜研究进展[J]. 中国生物工程杂志, 2004, 24(6):38-42.
[4]ABEL P P, NELSON R S, DE B, et al. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene[J]. Science, 1986, 232(4751): 738-743.
[5]FITCH M M M, MANSHARDT R M, GONSALVES D, et al. Transgenic papaya plants from Agrobacterium-mediated transformation of somatic embryos[J]. Plant Cell Reports, 1993, 12(5): 245-249.
[6]方静平. 基因枪遗传转化对番木瓜基因组结构和功能的影响[D].福州: 福建农林大学, 2016.
[7]TENNANT P, FERMIN G, FITCH M M, et al. Papaya ringspot virus resistance of transgenic Rainbow and SunUp is affected by gene dosage, plant development, and coat protein homology[J]. European Journal of Plant Pathology, 2001, 107(6): 645-653.
[8]RUAN X, LI H, ZHOU G. Evaluation of PRSV resistance of T2 transgenic papaya with replicase gene[J]. Journal of South China Agricultural University, 2004, 25(4):12-15.
[9]姜大刚,周峰,姚涓,等. 转基因番木瓜“华农一号”事件特异性定性PCR检测方法的建立[J]. 华南农业大学学报, 2009, 30(1):37-41.
[10]CHENG Y H, YEH S D. Construction and evaluation of transgenic tobacco plants expressing the coat protein gene of papaya ringspot virus with different translation leaders[J]. Botanical Bulletin of Academia Sinica, 2000, 41:1-10.
[11]BAU H J, CHENG Y H, YU T A, et al. Field evaluation of transgenic papaya lines carrying the coat protein gene of Papaya ringspot virus in Taiwan[J]. Plant Disease, 2004, 88(6): 594-599.
[12]WALL E M, LAWRENCE T S, GREEN M J, et al. Detection and identification of transgenic virus resistant papaya and squash by multiplex PCR[J]. European Food Research and Technology, 2004, 219(1): 90-96.
[13]王恒波,陈平华,陈如凯. 转基因植物及其产品PCR检测引物的筛选研究[J]. 热带农业科学, 2010, 30(11):10-14.
[14]韩建勋,陈红运,邓婷婷,等. 抗病毒转基因番木瓜的实时PCR检测[J]. 检验检疫学刊, 2010, 20(1):15-20.
[15]王立平,王东,龚熠欣,等. 国内外转基因农产品食用安全性研究进展与生产现状[J]. 中国农业科技导报, 2018, 20(3):94-103.

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备注/Memo

备注/Memo:
收稿日期:2018-05-04 基金项目:国家标准化管理委员会标准制修订项目(20100644-T-326、20100643-T-326) 作者简介:王恒波(1982-),男,陕西乾县人,硕士,助理研究员,主要从事分子检测研究。(Tel)0591-83789177;(E-mail)wanghengbo_0354@126.com 通讯作者:陈平华,(Tel)0591-83789177;(E-mail)50088945@qq.com
更新日期/Last Update: 2018-11-05