[1]王莹,李玉娟,李敏,等.紫叶紫薇新品系叶色变化转录组分析[J].江苏农业学报,2018,(05):1128-1137.[doi:doi:10.3969/j.issn.1000-4440.2018.05.023]
 WANG Ying,LI Yu-juan,LI Min,et al.Transcriptome analysis of a new strain of purple-leaf crape myrtle (Lagerstroemia indica) during leaves color changes[J].,2018,(05):1128-1137.[doi:doi:10.3969/j.issn.1000-4440.2018.05.023]
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紫叶紫薇新品系叶色变化转录组分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1128-1137
栏目:
园艺
出版日期:
2018-10-25

文章信息/Info

Title:
Transcriptome analysis of a new strain of purple-leaf crape myrtle (Lagerstroemia indica) during leaves color changes
作者:
王莹李玉娟李敏马祥建谈峰郭聪张健
(江苏沿江地区农业科学研究所,江苏南通226541)
Author(s):
WANG YingLI Yu-juanLI MinMA Xiang-jianTAN FengGUO congZHANG Jian
(Institute of Agricultural Sciences of Jiangsu Changjiang River Bank District, Nantong 226541, China)
关键词:
紫叶紫薇叶色变化转录组
Keywords:
purple-leaf Lagerstroemia indicaleaves color changestranscriptome
分类号:
S687.9
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.023
文献标志码:
A
摘要:
对紫叶紫薇新品系的叶片进行RNA-Seq测序和分析,共获取145 338条Contig,得到组装的Unigene有53 654条,平均长度1 186.25 bp。32 667条Unigene可以匹配到蛋白质数据库获得注释信息,KEGG和GO注释与富集分析显示,差异DEG主要调节激素信号转导、碳水化合物代谢、核糖体代谢等生物学功能。58个表达差异基因分别对比到毛果杨和拟南芥2个模式植物的数据库,筛出与叶绿素代谢相关候选基因9个,其中2个候选基因共同参与了叶绿素兼类黄酮代谢的调控,2个候选基因可能参与类黄酮代谢过程,它们分别属于DUF547、Polyketide_cyc2、Pkinase、Calreticulin、NAD_Gly3P_dh_N、Glyco_hydro_9等13个基因家族,主要经氧化还原反应、物质能量代谢、植物激素信号转导等机制介入紫薇叶色调控。本研究结果为探究紫叶紫薇叶色变异的分子机制提供参考。
Abstract:
This study was conducted on the transcription and bioinformatics analysis of a new strain of purple leaf crape myrtle (Lagerstroemia indica) during leaves color changes. A total of 145 338 contigs were obtained, and assembled de novo into 53 654 unigenes, with average length of 1 186.25 bp. KEGG and GO enrichment analysis results showed that differences in gene were significantly associated with some biological functions, mainly involved in the metabolism of the ribosome, carbohydrate metabolism, plant hormone signal transduction and other metabolic pathways. Total of nine unigenes involved in chlorophyll metabolism, two unigenes related to both chlorophyll metabolism and flavonoid metabolism and two unigenes involved in flavonoid metabolism were identified by comparing the 58 unigenes obtained with the GO and KEGG databases of Populus trichocarpa and Arabidopsis thaliana, which respectively belonged to 13 gene families including DUF547, Polyketide_cyc2, Pkinase, Calreticulin, NAD_Gly3P_dh_N, Glyco_hydro_9 etc. They mainly participated in the regulation of leaf color through the mechanism of oxidation-reduction reaction, energy metabolism, material metabolism, and plant hormone signal transduction. The experimental results provide a reference for the study of molecular mechanism of leaf color variation in purple-leaf crape myrtle.

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备注/Memo

备注/Memo:
收稿日期:2017-12-07 基金项目:南通市应用基础研究项目(MS12015075);中央财政农业技术推广项目[TG(16)048];南通市前沿与关键技术创新项目(MS22015039) 作者简介:王莹(1986-),女,江苏南通人,硕士,助理研究员,主要从事彩叶苗木品种引选、栽培方面的研究。(E-mail)466625450@qq.com 通讯作者:张健,(E-mail)yjnkyy@163.com
更新日期/Last Update: 2018-11-05