[1]张善磊,孙旭超,陈涛,等.Pi-ta、Pi-5、Pi-km和Pi-b基因在粳稻品种（系）中的分布及对穗颈瘟的抗性[J].江苏农业学报,2018,(05):961-971.[doi:doi:10.3969/j.issn.1000-4440.2018.05.001]
　ZHANG Shan-lei,SUN Xu-chao,CHEN Tao,et al.Distribution of Pi-ta，Pi-5，Pi-km and Pi-b genes in japonica rice varieties (lines) and their relationship with neck blast resistance[J].,2018,(05):961-971.[doi:doi:10.3969/j.issn.1000-4440.2018.05.001]
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Pi-ta、Pi-5、Pi-km和Pi-b基因在粳稻品种（系）中的分布及对穗颈瘟的抗性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
961-971
栏目:
遗传育种·生理生化
出版日期:
2018-10-25

文章信息/Info

Title:
Distribution of Pi-ta，Pi-5，Pi-km and Pi-b genes in japonica rice varieties (lines) and their relationship with neck blast resistance
作者:
张善磊; 孙旭超; 陈涛; 赵春芳; 朱镇; 赵庆勇; 周丽慧; 赵凌; 姚姝; 王才林
(江苏省农业科学院粮食作物研究所/ 江苏省优质水稻工程技术研究中心/ 国家水稻改良中心南京分中心,江苏南京210014)
Author(s):
ZHANG Shan-lei; SUN Xu-chao; CHEN Tao; ZHAO Chun-fang; ZHU Zhen; ZHAO Qing-yong; ZHOU Li-hui; ZHAO Ling; YAO Shu; WANG Cai-lin
(Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China)
关键词:
粳稻; 穗颈瘟; 抗性基因; 分子标记
Keywords:
japonica rice; neck blast; resistance genes; molecular marker
分类号:
S511.2+20.34
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.001
文献标志码:
A
摘要:
利用稻瘟病抗性基因Pi-ta、Pi-5、Pi-km、Pi-b的功能标记，对国内外238份粳稻品种(系)进行基因检测，结合穗颈瘟鉴定结果，明确4个抗性基因的区域分布特点，评估其对穗颈瘟的抗性效果。Pi-ta和Pi-b主要分布在东北、长江中下游及西南地区品种(系)中，Pi-5和Pi-km在西南地区品种中的分布高于其他地区。238份粳稻品种(系)中含Pi-ta、Pi-5、Pi-km、Pi-b单个抗性基因的品种(系)分别有33、30、29和38份；含2个抗性基因的品种(系)共有66份，其中含Pi-ta/Pi-b的品种(系)最多，有28份；同时含3个抗性基因的品种(系)有12份；4个基因均不含有的品种(系)有30份。Pi-ta对穗颈瘟的抗性效果最好，感病率为0，病情指数为22.73；其次是Pi-5和Pi-km基因，病情指数分别为59.17和70.69；Pi-b对穗颈瘟的抗性效果最差，感病率为86.84%，病情指数为83.55。双基因组合对穗颈瘟的抗性效果依次为：Pi-ta/Pi-5>Pi-ta/Pi-km>Pi-ta/Pi-b>Pi-5/Pi-km；3份同时含有Pi-ta、Pi-5、Pi-km 3基因的品种表现出最强的穗颈瘟抗性。
Abstract:
To state the region distribution of Pi-ta, Pi-5, Pi-km, Pi-b genes and their relationship with neck blast resistance, 238 japonica rice varieties (lines) from different regions of China and abroad were genotyped using the functional markers of the four genes and their disease reactions were detected to evaluate the effects of these genes on the resistance of neck blast. Pi-ta and Pi-b distributed in the Northeast, the middle and lower reaches of the Yangtze River and Southwest of China, while Pi-5 and Pi-km were more inclined to the Southwest China than other regions. Moreover, 33, 30, 29 and 38 varieties (lines) were detected to carry the single gene of Pi-ta, Pi-5, Pi-km and Pi-b, respectively. A total of 66 varieties (lines) carried two resistance genes and there were 28 varieties (lines) harboring Pi-ta/Pi-b which was much higher than other gene combinations. There were 12 varieties (lines) carrying three resistance genes Pi-ta, Pi-5 and Pi-km. Thirty varieties (lines) were detected with no resistance gene. Pi-ta gene had the best effects to neck blast resistance with a prevalence of 0 and a disease index of 22.73, followed by Pi-5 and Pi-km genes with a disease index of 59.17 and 70.69, respectively. The resistance of Pi-b gene was the lowest among them with a prevalence of 86.84 and a disease index of 83.55. The effects of double genes to neck blast resistance showed that Pi-ta/Pi-5>Pi-ta/Pi-km>Pi-ta/Pi-b>Pi-5/Pi-km. Three varieties containing Pi-ta/Pi-5/Pi-km genes were detected and showed the strongest effects to neck blast resistance.

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备注/Memo

备注/Memo:
收稿日期：2018-02-02 基金项目：国家科技支撑计划项目（2015BAD01B02）；江苏省重点研发项目（BF2016370）；江苏省农业科技自主创新基金项目[SCX(17)1063]；现代农业产业技术体系项目（CARS-01-62） 作者简介：张善磊(1990-)，男，安徽六安人，硕士研究生，主要从事水稻遗传育种研究。(Tel)18801586930；(E-mail)415407262@qq.com 通讯作者：王才林,(Tel)025-84390317；(E-mail)clwang@jaas.ac.cn
更新日期/Last Update: 2018-11-05